Colorimetric Sensor Array Based on Gold Nanoparticles with Diverse Surface Charges for Microorganisms Identification

Bingyu, Li; Li, Xizhe; Dong, Yanhua; Wang, Bing; Li, Dongyang; Shi, Y. Z.; Wu, Yayan

doi:10.1021/acs.analchem.7b02594

Cited by 122 publications

(74 citation statements)

References 26 publications

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“…Over the last decade, the ‘chemical‐nose’ approach has been successfully applied to sense a wide range of analytes. As an example, it can be mentioned the proteins, cells and bacterias up to multianion discrimination . This ‘nose’ ‐based strategy requires different receptors, which are generally obtained through surface engineering of NPs .…”

Section: Introductionmentioning

confidence: 99%

Fluorescence Based Platform to Discriminate Protein Using Carbon Quantum Dots

et al. 2019

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There is an urgent demand to develop a cheap, fast and robust methodology to sense proteins, since these biomolecules are often used as biomarker responsible for diagnosing of some diseases, such as cancer. In this regard, we report a theoretical and experimental study, as well as a cheap and effective ‘chemical‐nose’ strategy based on carbon quantum dots (CQDs) and metallic cations (M) to discriminate proteins at concentration as low as 50 nM. Thus, the CQDs were firstly synthesized through citric acid thermolysis and their characteristics were fully investigated by UV‐Vis absorption, fluorescence, infrared (FTIR), XPS and Raman spectroscopies and atomic force microscopy (AFM). These results pointed out for quasi‐spherical CQDs with diameters in the range of 1.2‐7 nm, presence of stacked graphitic layers and oxygenated functional groups, as well as disordered carbon. Based on the structural and morphological features, computational simulations were carried out to obtain a better understanding of the atomic structure. Our results evidenced a carbon‐based nanoparticle formed by stacked graphene nanoflakes containing defects due to the presence of functional groups within the graphene layers. Afterwards, a ‘tongue’‐based approach was developed by using three distinct CQDs – M (M=Fe3+, Cu2+ or Ni2+) ensembles, which allowed us to acquire different and reproducible fluorescence patterns for four proteins (bovine serum albumin, hemoglobin, myoglobin and cytochrome C) at 50 nM. Subsequently, the pattern recognition was performed using linear discriminant analysis and 36 samples were correctly identified affording 100% of accuracy.

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Section: Introductionmentioning

confidence: 99%

Fluorescence Based Platform to Discriminate Protein Using Carbon Quantum Dots

et al. 2019

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“…This phenomenon causes a sharp and intense absorption band in the visible range, which can be readily tuned by varying the particle size, shape, and the surrounding physicochemical environment [ 8 ]. Thus, label-free colorimetric sensors based on AuNPs (nanobiosensors) have been widely proposed as a promising analytical for selective binding and detection of chemical and biological targets, including metal ions [ 9 ], antibiotics [ 10 ], mycotoxins [ 11 ], as well as a large number of microorganisms [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Slight pH Fluctuations in the Gold Nanoparticle Synthesis Process Influence the Performance of the Citrate Reduction Method

Contreras-Trigo

Díaz-García

Guzmán-Gutiérrez

et al. 2018

Sensors

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Gold nanoparticles (AuNPs) are currently under intense investigation for biomedical and biotechnology applications, thanks to their ease in preparation, stability, biocompatibility, multiple surface functionalities, and size-dependent optical properties. The most commonly used method for AuNP synthesis in aqueous solution is the reduction of tetrachloroauric acid (HAuCl4) with trisodium citrate. We have observed variations in the pH and in the concentration of the gold colloidal suspension synthesized under standard conditions, verifying a reduction in the reaction yield by around 46% from pH 5.3 (2.4 nM) to pH 4.7 (1.29 nM). Citrate-capped AuNPs were characterized by UV-visible spectroscopy, TEM, EDS, and zeta-potential measurements, revealing a linear correlation between pH and the concentration of the generated AuNPs. This result can be attributed to the adverse effect of protons both on citrate oxidation and on citrate adsorption onto the gold surface, which is required to form the stabilization layer. Overall, this study provides insight into the effect of the pH over the synthesis performance of the method, which would be of particular interest from the point of view of large-scale manufacturing processes.

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“…Durch Modifikation der AuNP‐Oberfläche mit kreuzreaktiven, bindenden Komponenten kann dieser selektive Farbwechsel mit Gruppen von Analyten induziert werden. Die Implementierung dieser Transduktionsstrategie wurde durch die Detektion von bakteriellen Spezies, toxischen Metallionen und Proteinen demonstriert. Vor kurzem wurde eine dritte Variante kolometrischer Arrays vorgestellt, die Opal‐ähnliche photonische Kristallstrukturen anwendet, die starke, auf ihrer Chemie und ihrer Interaktion mit verschiedenen Analyten basierende Farbveränderungen aufweisen (Abbildung ) …”

Section: Design Konstruktion Und Analyse Von Arraysunclassified

Array‐basierte Sensorik mit der “chemischen Nase” in der Diagnostik und Wirkstoffentdeckung

2019

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Array‐basierte Sensorplattformen – “Chemische Nasen/Zungen” – sind inspiriert vom olfaktorischen System von Säugetieren. Multiple Sensorelemente in diesen Geräten interagieren selektiv mit Zielanalyten und produzieren dabei ein spezifisches Antwortmuster, wodurch sie die Identifikation von Analyten ermöglichen. Dieser Ansatz bietet einzigartige Möglichkeiten im Vergleich mit “traditionellen”, hoch‐spezifischen Sensorelementen, z. B. Antikörpern. Array‐basierte Sensoren sind herausragend, wenn es um die Identifikation kleiner Veränderungen in komplexen Mischungen geht, und ebendiese Fähigkeit wird nun in der chemischen Biologie und klinischen Pathologie genutzt, ermöglicht durch ein vielfältiges Instrumentarium neuer Molekular‐, Biokonjugat‐ und Nanomaterial‐Techniken. Innovationen beim Design und der Analyse der Arrays stellen ein robustes Instrumentarium für moderne biomedizinische Zielsetzungen, einschließlich der Präzisionsmedizin, zur Verfügung.

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Colorimetric Sensor Array Based on Gold Nanoparticles with Diverse Surface Charges for Microorganisms Identification

Cited by 122 publications

References 26 publications

Fluorescence Based Platform to Discriminate Protein Using Carbon Quantum Dots

Fluorescence Based Platform to Discriminate Protein Using Carbon Quantum Dots

Slight pH Fluctuations in the Gold Nanoparticle Synthesis Process Influence the Performance of the Citrate Reduction Method

Array‐basierte Sensorik mit der “chemischen Nase” in der Diagnostik und Wirkstoffentdeckung

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