Colorimetric Technique for Antimony Detection Based on the Use of Gold Nanoparticles Conjugated with Poly-A Oligonucleotide

Berlina, Anna N.; Komova, Nadezhda S.; Zherdev, Anatoly V.; Gaur, M. S.; Dzantiev, Boris B.

doi:10.3390/app9224782

Cited by 10 publications

(8 citation statements)

References 53 publications

(55 reference statements)

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“…Keterangan: A. Nilai absorbansi koloid emas dengan Koloid emas hasil sintesis dengan metode ini digunakan sebagai label pada strip imunokromatografi yang dikembangkan. Karakteristik koloid emas dengan puncak serapan pada panjang gelombang 525 nm dengan ukuran partikel 30 nm telah dilaporkan (Rahme et al, 2013;Zeng et al, 2014;Berlina et al, 2019) dan banyak peneliti menggunakan koloid emas dengan ukuran partikel tersebut sebagai label pada pembuatan strip imunokromatografi (Sun et al, 2012;Liu et al, 2014;Ariffin et al, 2020). Koloid emas dengan ukuran partikel 31,88 nm pada penelitian ini memberikan kestabilan yang baik ketika dikonjugasikan dengan jumlah optimum antibodi anti A. hydrophila.…”

Section: Pembahasanunclassified

Preparasi Strip Imunokromatografi Koloid Emas untuk Deteksi Cepat Aeromonas hydrophila

Suherman¹,

Damayanti

Indrawati

2020

Acta Vet Indones

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Aeromonas hydrophila merupakan agen penyebab Motile Aeromonas Septicemia (MAS) yang menyebabkan kerugian ekonomi pada industri akuakultur air tawar. Saat ini, metode diagnostik yang tersedia untuk determinasi A. hydrophila membutuhkan waktu lama dan tidak sesuai diaplikasikan di lapangan. Oleh karena itu, dibutuhkan adanya metode lain yang dapat digunakan sebagai diagnostik yang cepat dan aplikatif di lapangan. Tujuan dari penelitian ini adalah membuat alat diagnostik yang mampu digunakan sebagai pendeteksi agen penyebab MAS. Secara singkat, partikel koloid emas berukuran 31,88 nm dibuat melalui reduksi kimia asam kloroaurat dengan natrium sitrat. Koloid emas sebagai detektor dikonjugasikan dengan antibodi poliklonal anti-A. hydrophila 50 μg/ml pada pH 7. Membran nitroselulosa sebagai membran reaksi, ditetesi dengan antibodi poliklonal anti-A. hydrophila 2 mg/ml di garis T dan antibodi goat anti-rabbit IgG 1 mg/ml di garis C. Hasil dari penelitian ini, strip imunokromatografi yang dikembangkan mampu mendeteksi A. hydrophila dengan deteksi minimum 1,2x105 CFU/ml. Strip bersifat spesifik terhadap A. hydrophila, tidak ada reaksi silang yang ditemukan ketika direaksikan dengan bakteri lain. Analisis sampel menggunakan strip hanya membutuhkan waktu 10 menit. Strip imunokromatografi yang dikembangkan dapat mendeteksi A. hydrophila dengan cepat dan memiliki potensi untuk digunakan di lapangan.

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Section: Pembahasanunclassified

Preparasi Strip Imunokromatografi Koloid Emas untuk Deteksi Cepat Aeromonas hydrophila

Suherman¹,

Damayanti

Indrawati

2020

Acta Vet Indones

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“…Currently, colorimetric methods deserve special attention as they offer a visual assessment of color change, allowing an easy readout of analytical signals. Colorimetric techniques for Sb 3+ detection using indicator dyes [14][15][16] and nanoparticles [17][18][19] were developed. The application of dyes such as bis [2-(5-chloro-2-pyridylazo)-5-diethylaminophenola to]cobalt(III), bromopyrogallol red and pyrene causes the formation of complexes with Sb 3+ and is followed by photo-or fluorometric detection.…”

Section: Introductionmentioning

confidence: 99%

“…In particular, a homogeneous colorimetric technique for the detection of Sb 3+ based on the aggregation of aptamer-modified gold nanoparticles, accompanied by a rapid color transition from pink to blue, was developed [19]. Aptamer A 10 was used as the receptor molecule, forming a complex with Sb 3+ due to interaction with hydroxyl groups of adenine residues [25,26].…”

Section: Introductionmentioning

confidence: 99%

Sensitive Silver-Enhanced Microplate Apta-Enzyme Assay of Sb3+ Ions in Drinking and Natural Waters

Komova,

Serebrennikova,

Berlina

et al. 2023

Molecules

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The toxic effects of antimony pose risks to human health. Therefore, simple analytical techniques for its widescale monitoring in water sources are in demand. In this study, a sensitive microplate apta-enzyme assay for Sb3+ detection was developed. The biotinylated aptamer A10 was hybridized with its complementary biotinylated oligonucleotide T10 and then immobilized on the surface of polysterene microplate wells. Streptavidin labeled with horseradish peroxidase (HRP) bound to the biotin of a complementary complex and transformed the 3,3′,5,5′-tetramethylbenzidine substrate, generating an optical signal. Sb3+ presenting in the sample bounded to an A10 aptamer, thus releasing T10, preventing streptavidin-HRP binding and, as a result, reducing the optical signal. This effect allowed for the detection of Sb3+ with a working range from 0.09 to 2.3 µg/mL and detection limit of 42 ng/mL. It was established that the presence of Ag+ at the stage of A10/T10 complex formation promoted dehybridization of the aptamer A10 and the formation of the A10/Sb3+ complex. The working range of the Ag+-enhanced microplate apta-enzyme assay for Sb3+ was determined to be 8–135 ng/mL, with a detection limit of 1.9 ng/mL. The proposed enhanced approach demonstrated excellent selectivity against other cations/anions, and its practical applicability was confirmed through an analysis of drinking and spring water samples with recoveries of Sb3+ in the range of 109.0–126.2% and 99.6–106.1%, respectively.

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“…The implementation of nanomaterials into the development of colorimetric systems makes it possible to improve the sensitivity of the determination of toxins, as well as the accuracy of the analysis. The most common substrate that is used in colorimetric analysis is metal nanoparticles, especially silver [24,25] and gold nanoparticles (AuNPs) [26][27][28], due to their controllable morphology, chemical properties, and strong surface plasmon resonance (SPR). The ability of AuNPs to change color in response to changes in particle size and interparticle space, which is recorded spectrophotometrically as a shift in the absorption peak, makes them an ideal colorimetric sensing probe [28,29].…”

Section: Introductionmentioning

confidence: 99%

“…The most common substrate that is used in colorimetric analysis is metal nanoparticles, especially silver [24,25] and gold nanoparticles (AuNPs) [26][27][28], due to their controllable morphology, chemical properties, and strong surface plasmon resonance (SPR). The ability of AuNPs to change color in response to changes in particle size and interparticle space, which is recorded spectrophotometrically as a shift in the absorption peak, makes them an ideal colorimetric sensing probe [28,29]. Previously described work [30] demonstrated the use of native citrate-stabilized gold nanoparticles for the simultaneous detection of several ions.…”

Section: Introductionmentioning

confidence: 99%

Mercaptosuccinic-Acid-Functionalized Gold Nanoparticles for Highly Sensitive Colorimetric Sensing of Fe(III) Ions

et al. 2021

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The development of reliable and highly sensitive methods for heavy metal detection is a critical task for protecting the environment and human health. In this study, a qualitative colorimetric sensor that used mercaptosuccinic-acid-functionalized gold nanoparticles (MSA-AuNPs) to detect trace amounts of Fe(III) ions was developed. MSA-AuNPs were prepared using a one-step reaction, where mercaptosuccinic acid (MSA) was used for both stabilization, which was provided by the presence of two carboxyl groups, and functionalization of the gold nanoparticle (AuNP) surface. The chelating properties of MSA in the presence of Fe(III) ions and the concentration-dependent aggregation of AuNPs showed the effectiveness of MSA-AuNPs as a sensing probe with the use of an absorbance ratio of A530/A650 as an analytical signal in the developed qualitative assay. Furthermore, the obvious Fe(III)-dependent change in the color of the MSA-AuNP solution from red to gray-blue made it possible to visually assess the metal content in a concentration above the detection limit with an assay time of less than 1 min. The detection limit that was achieved (23 ng/mL) using the proposed colorimetric sensor is more than 10 times lower than the maximum allowable concentration for drinking water defined by the World Health Organization (WHO). The MSA-AuNPs were successfully applied for Fe(III) determination in tap, spring, and drinking water, with a recovery range from 89.6 to 126%. Thus, the practicality of the MSA-AuNP-based sensor and its potential for detecting Fe(III) in real water samples were confirmed by the rapidity of testing and its high sensitivity and selectivity in the presence of competing metal ions.

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Colorimetric Technique for Antimony Detection Based on the Use of Gold Nanoparticles Conjugated with Poly-A Oligonucleotide

Cited by 10 publications

References 53 publications

Preparasi Strip Imunokromatografi Koloid Emas untuk Deteksi Cepat Aeromonas hydrophila

Preparasi Strip Imunokromatografi Koloid Emas untuk Deteksi Cepat Aeromonas hydrophila

Sensitive Silver-Enhanced Microplate Apta-Enzyme Assay of Sb3+ Ions in Drinking and Natural Waters

Mercaptosuccinic-Acid-Functionalized Gold Nanoparticles for Highly Sensitive Colorimetric Sensing of Fe(III) Ions

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