Colorimetry and Thin-Layer Chromatography of Sialic Acids

Schauer, Roland; Corfield, Anthony P.

doi:10.1007/978-3-7091-8680-0_5

Cited by 18 publications

(13 citation statements)

References 94 publications

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“…In the present study, serum sialic acid levels were significantly raised in osteosarcoma and group II patients as compared to control group I. These findings are in agreement with those reported in literature [24,25]. A positive correlation was observed between rise in sialic acid levels and fluoride levels, although the difference was not statistically significant (r=0.00017, p<0.05).…”

Section: Discussionsupporting

confidence: 95%

Serum Fluoride and Sialic Acid Levels in Osteosarcoma

Sandhu

Lal

Kundu

et al. 2009

Biol Trace Elem Res

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Osteosarcoma is a rare malignant bone tumor most commonly occurring in children and young adults presenting with painful swelling. Various etiological factors for osteosarcoma are ionizing radiation, family history of bone disorders and cancer, chemicals (fluoride, beryllium, and vinyl chloride), and viruses. Status of fluoride levels in serum of osteosarcoma is still not clear. Recent reports have indicated that there is a link between fluoride exposure and osteosarcoma. Glycoproteins and glycosaminoglycans are an integral part of bone and prolonged exposure to fluoride for long duration has been shown to cause degradation of collagen and ground substance in bones. The present study was planned to analyze serum fluoride, sialic acid, calcium, phosphorus, and alkaline phosphatase levels in 25 patients of osteosarcoma and age- and sex-matched subjects with bone-forming tumours other than osteosarcoma and musculo-skeletal pain (controls, 25 each). Fluoride levels were analyzed by ISE and sialic acid was analyzed by Warren's method. Mean serum fluoride concentration was found to be significantly higher in patients with osteosarcoma as compared to the other two groups. The mean value of flouride in patients with other bone-forming tumors was approximately 50% of the group of osteosarcoma; however, it was significantly higher when compared with patients of group I. Serum sialic acid concentration was found to be significantly raised in patients with osteosarcoma as well as in the group with other bone-forming tumors as compared to the group of controls. There was, however, no significant difference in the group of patients of osteosarcoma when compared with group of patients with other bone-forming tumors. These results showing higher level of fluoride with osteosarcoma compared to others suggesting a role of fluoride in the disease.

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Section: Discussionsupporting

confidence: 95%

Serum Fluoride and Sialic Acid Levels in Osteosarcoma

Sandhu

Lal

Kundu

et al. 2009

Biol Trace Elem Res

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“…Thin-layer chromatography of urinary oligosaccharides was performed on TLC sheets coated with silica using the solvent system n-propanol-acetic acid-water (85:1:I5 v/v). The chromatograms were either stained with orcinol:sulphuric acid for the detection of hexose-containing compounds (Dorland et al, 1986), or with the Ehrlich reagent for the detection of carbamidocontaining compounds (Bremer et al, 1981), or with orcinol-FeCl3-HC1 to visualize sialic acid-containing compounds (Schauer and Corfield, 1982). Carbohydrate composition analysis was carried out by gas chromatography according to the method of Kamerling et al (1975).…”

Section: Methodsmentioning

confidence: 99%

Novel storage products in humanβ‐mannosidosis

Hokke

Durán

Dorland

et al. 1990

J of Inher Metab Disea

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Lysosomal fl-mannosidase (EC 3.2.1.25) is the final enzyme involved in the catabolism of the oligosaccharide side-chains of glycoproteins. Following the description of caprine fl-mannosidosis in the early 1980s, several human patients with a deficiency of this enzyme were described (Cooper et al., 1986;Wenger et al., 1986;Dorland et at., 1988). Defects in lysosomal enzymes usually lead to storage and urinary excretion of incompletely catabolized oligosaccharides. Identification of these urinary excretion products may be helpful in establishing the diagnosis. In human fl-mannosidosis only an abnormal amount of the disaccharide mannosyl-fi(1--.4)-N-acetylglucosamine (Manfi(1-~4)GlcNAc) has been reported so far, in contrast with the much larger and more complex oligosaccharides observed in a-mannosidosis.Generally the structures of the excreted metabolites are those predicted based on the inherited deficiencies of specific exoglycosidases and the coincident action of an endo-fl-N-acetylglucosaminidase which cleaves the linkage between the two GlcNAc residues of the di-N-acetylchitobiose core. Here we describe the occurrence of a sialyloligosaccharide which is probably formed by enzymatic synthesis from the storage product Manfl(1 ~4)GlcNAc as substrate. Furthermore, the characterization is described of a urinary carbohydrate-urea conjugate. PATIENTSThe clinical histories of A.B. and H.B., two brothers born to a consanguineous Turkish couple, have been described before (Dorland et al., 1988). Recently another male child was born in this family. Analysis of oligosaccharides in urine as well as measurement of fl-mannosidase in plasma yielded entirely normal results. METHODSOligosaccharides were isolated from the patients' urine by the following sequence of techniques: concentration by rotary evaporation or lyophilization, cation-and anionexchange purification, gel filtration chromatography on Bio-Gel P2, and preparative 273

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“…Solvent n-propanol-water (7:3v/v); staining with resorcinol/Cu z+ (Schauer and Corfield, 1982). Lane 1: total urine of nISSD, Case 1; lanes 2-6: fractions 1-5.…”

Section: Excretion Of Sialyloligosaccharides Free Sialic Acidsmentioning

confidence: 99%

Storage material from urine and tissues in the nephropathic phenotype of infantile sialic acid storage disease

Paschke

Gruber

Ring

et al. 1991

J of Inher Metab Disea

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We analysed urine and tissue specimens from two nephrotic infantile sialic acid storage disease patients (nISSD) for free and bound sialic acids in comparison to non-nephrotic ISSD patients (ISSD), patients with minimal change nephrosis (nControl) and normal controls (Control). No differences in the excretion of urinary free sialic acid could be detected between ISSD and nISSD urines. Sialyloligosaccharide fractions were only slightly elevated and of apparently normal composition. Owing to glomerular dysfunction, measurable quantities of protein-bound sialic acids were present in nISSD and nControl. In nISSD tissues, free sialic acid was elevated 18-100-fold above control and 3-12-fold above Niemann-Pick A (NPA) samples. The storage of membrane-bound sialic acid was slightly increased compared to control tissues, but equal to those from NPA, thus reflecting an unspecific increase of membranes due to lysosomal storage. According to these results no major biochemical differences were detectable between ISSD and nISSD. The nephrotic syndrome in nISSD could not be related to a general deficit in the sialylation of glycoproteins. Nevertheless, a cell membrane-specific alteration in sialoglycoproteins of glomerular cells might still be possible.

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Colorimetry and Thin-Layer Chromatography of Sialic Acids

Cited by 18 publications

References 94 publications

Serum Fluoride and Sialic Acid Levels in Osteosarcoma

Serum Fluoride and Sialic Acid Levels in Osteosarcoma

Novel storage products in humanβ‐mannosidosis

Storage material from urine and tissues in the nephropathic phenotype of infantile sialic acid storage disease

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