Colorimetry: Choice of colorimetric parameters for chromophore concentration measurements

Kumpanenko, I. V.; Рощин, А. В.; Иванова, Н. А.; Bloshenko, A. V.; Shalynina, N. A.; Korneeva, T. N.

doi:10.1134/s1070363214110498

Cited by 6 publications

(4 citation statements)

References 3 publications

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“…For example, arsenic (3+) in water samples [ 1 ], cobalt (2+) in water samples [ 2 ] and nutrients in corn [ 3 ] can be assayed by colorimetry. Compared to other spectroscopic techniques, colorimetry provides more complex but not easy to process data [ 4 ]. When a Red–Green–Blue (RGB) color model is used independent values of the color channels can be obtained for the assay.…”

Section: Introductionmentioning

confidence: 99%

Photography by Cameras Integrated in Smartphones as a Tool for Analytical Chemistry Represented by an Butyrylcholinesterase Activity Assay

Pohanka

2015

Sensors

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Smartphones are popular devices frequently equipped with sensitive sensors and great computational ability. Despite the widespread availability of smartphones, practical uses in analytical chemistry are limited, though some papers have proposed promising applications. In the present paper, a smartphone is used as a tool for the determination of cholinesterasemia i.e., the determination of a biochemical marker butyrylcholinesterase (BChE). The work should demonstrate suitability of a smartphone-integrated camera for analytical purposes. Paper strips soaked with indoxylacetate were used for the determination of BChE activity, while the standard Ellman’s assay was used as a reference measurement. In the smartphone-based assay, BChE converted indoxylacetate to indigo blue and coloration was photographed using the phone’s integrated camera. A RGB color model was analyzed and color values for the individual color channels were determined. The assay was verified using plasma samples and samples containing pure BChE, and validated using Ellmans’s assay. The smartphone assay was proved to be reliable and applicable for routine diagnoses where BChE serves as a marker (liver function tests; some poisonings, etc.). It can be concluded that the assay is expected to be of practical applicability because of the results’ relevance.

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Section: Introductionmentioning

confidence: 99%

Photography by Cameras Integrated in Smartphones as a Tool for Analytical Chemistry Represented by an Butyrylcholinesterase Activity Assay

Pohanka

2015

Sensors

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“…The results of our study indicate that identical anthocyanins are characteristic for all flower segments of a particular cultivar, but their content differs significantly among the DS, PS, and CT. Generally, DS accumulated highest levels of total anthocyanins with the exception of the PS of the pink-colored hybrid. The latter was also the darkest part of the pink flower according to the parameter L*, which is in tight correlation with anthocyanin composition of plant tissue (Kumpanenko et al, 2014;Schmitzer et al, 2010).…”

Section: Colorimetric Parameters Andmentioning

confidence: 62%

“…Differences in color parameters were significant among the three flower segments, and most particularly, the CT was characterized by highest L* and lowest a*. The latter marks green to purple hues of a plant sample and is higher in red, pink, or blue flowers (Kumpanenko et al, 2014;Schmitzer et al, 2010). It is therefore not surprising that DS of the red and PS of the pink hybrid primrose flowers were defined by highest a*.…”

Section: Colorimetric Parameters Andmentioning

confidence: 92%

“…In CIE (Commission Internationale de l' Eclairage) L* a* b* system of color representation, the L* value corresponds to a dark-bright scale and represents the relative lightness of the sample (0 = black, 100 = white). The coordinates a* and b* vary from -128 to +128 and relate to green to purple and blue to yellow hues, respectively (Kumpanenko et al, 2014). The hue angle (h°) is expressed in degrees from 0 to 360 (0°= red, 90°= yellow, 180°= green, and 360°= blue).…”

Section: Methodsmentioning

confidence: 99%

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Phenolic Accumulation in Hybrid Primrose and Pigment Distribution in Different Flower Segments

Schmitzer¹,

Mikulič-Petkovšek²,

Štampar³

et al. 2017

J. Amer. Soc. Hort. Sci.

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Detailed anthocyanin and flavonol profiles were investigated in three flower segments of four different hybrid primrose (Primula ×polyantha) cultivars, and individual compounds were identified using high-performance liquid chromatography (HPLC)/mass spectrometry system. Chlorophyll a and b and total carotenoids were evaluated spectrophotometrically in the corolla tube (CT), and distal and proximal flower segments, and the color of each segment was assessed with a colorimeter. Chlorophyll b predominated over chlorophyll a in all flower segments, and the highest total chlorophyll levels were found in the CTs. Sixteen different anthocyanins (glycosides of cyanidin, delphinidin, peonidin, petunidin, malvidin, and rosinidin) were identified in red, pink, and blue flower extracts. Distal segments of the red hybrid and proximal segments of the pink hybrid accumulated highest levels of total anthocyanins, and no red pigments were detected in yellow-flowered hybrid primrose. Six groups of flavonols (40 individual compounds in total) were detected in different flower segments of four hybrid primrose cultivars. Yellow primrose was characterized by the greatest diversity of flavonols as it contained four isorhamnetin, five kaempferol, six laricitrin, three myricetin, six quercetin, and six syringetin glycosides. On the other hand, the smallest variety of flavonols was detected in pink hybrids. Total phenolic content (TPC) was lowest in the CT (yellow > red > pink), significantly higher in the proximal flower segment (yellow > red > pink), and highest in the distal part of the primrose petal (yellow > pink > red).

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Rapid Determination of Nitrate Ions in Drinking Water Based on Image Processing Techniques Using a Smartphone Platform

Farahani,

Tashakori,

Shadman

2023

Preprint

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Colorimetry: Choice of colorimetric parameters for chromophore concentration measurements

Cited by 6 publications

References 3 publications

Photography by Cameras Integrated in Smartphones as a Tool for Analytical Chemistry Represented by an Butyrylcholinesterase Activity Assay

Photography by Cameras Integrated in Smartphones as a Tool for Analytical Chemistry Represented by an Butyrylcholinesterase Activity Assay

Phenolic Accumulation in Hybrid Primrose and Pigment Distribution in Different Flower Segments

Rapid Determination of Nitrate Ions in Drinking Water Based on Image Processing Techniques Using a Smartphone Platform

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