Colour development of immunogold-labelled antibodies for light microscopy

Fritz, Péter; Hoenes, J; Schenk, J.; Mischlinski, A; Grau, Andreas; Saal, J. G.; Tuczek, H. V.; Multhaupt, Hinke A.B.; Pfleiderer, Gerhard

doi:10.1007/bf00500630

Cited by 3 publications

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“…Polyacrylamide gel electrophoresis (PAGE) is a widely used method for the analysis of proteins due to its high resolution, capability to handle multiple samples simultaneously, ability to analyze a trace amount of protein and modest cost. [1][2][3][4] However, the quantification of an exceedingly low level of proteins in PAGE is still a major challenge.…”

Section: Introductionmentioning

confidence: 99%

An amine-reactive tetraphenylethylene derivative for protein detection in SDS-PAGE

Qin

Feng

et al. 2012

Analyst

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A new aggregation-induced emission (AIE) compound 1,2-bis[4-(isothiocyanatomethyl)phenyl]-1,2-diphenylethene (2) was synthesized for use in SDS-PAGE. The molecule is practically nonemissive in solution but becomes highly emissive after reacting with the amine groups of the proteins by either the prestaining or poststaining method. The sensitivity of 2 achieved in the prestaining method is the same as that of Coomassie brilliant blue (CBB), while that observed in the poststaining method is higher than that of CBB. Excellent linear responses with the amount of protein were obtained in both cases. The detection of a mixture of proteins with different molecular weights was successfully achieved.

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Section: Introductionmentioning

confidence: 99%

An amine-reactive tetraphenylethylene derivative for protein detection in SDS-PAGE

Qin

Feng

et al. 2012

Analyst

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A double protein A-gold-silver staining method for tissue antigens in light microscopy

Fujimori

1992

Histochem J

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A double staining method has been developed for light microscopic immunohistochemistry. The technique consists of a sequence of protein A-gold-silver procedures, in which the reaction products are visualized in black and brown colours. The results obtained in the rat pancreatic islets and adenohyophysis, in combination with a variety of histochemical controls, substantiate both the specificity and reliability of this method. The double staining method is simple and economical, since the two differently coloured reaction products are obtained by a common mechanism of colouration (physical development) using a single probe, protein A-gold.

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Silver-Enhanced Colloidal Gold Method

Scopsi

1989

Colloidal Gold

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Colour development of immunogold-labelled antibodies for light microscopy

Cited by 3 publications

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An amine-reactive tetraphenylethylene derivative for protein detection in SDS-PAGE

An amine-reactive tetraphenylethylene derivative for protein detection in SDS-PAGE

A double protein A-gold-silver staining method for tissue antigens in light microscopy

Silver-Enhanced Colloidal Gold Method

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