Colour Reaction Screening of Clones With Diacetyl and Acetoin Reductase Activities

Abstract

A genomic library of the chromosomal DNA of a brewing strain (Saccharomyces carlsbergensis) has been made in a Escherichia co//HB101 strain as host. A coloured test allowing one to isolate recombined clones possessing diacetyl (DR) and acetoin (AR) reductase activities is described.This test allowed one to detect 3 Escherichia co//clones having both activities (DR and AR) from among 3000 E. coli strains constituting the genomic library of Saccharomyces carlsbergensis in E. coli.