Column experiments for microbiological treatment of acid mine drainage: low-temperature, low-pH and matrix investigations

Tsukamoto, Tokihiro; Killion, H.A; Miller, Glenn C.

doi:10.1016/j.watres.2003.12.012

Cited by 155 publications

(98 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…SRB recycle much of the sedimentary organic carbon pool in marsh sediments (42)(43)(44) and indirectly inhibit production of the greenhouse gas methane (37,71). They can restrict the mobility of dissolved contaminant metals by inducing precipitation of poorly soluble metal sulfides, and studies have examined their use in constructed wetlands to bioremediate acid mine drainage (AMD) and other metalliferous waste streams (11,35,40,46,50,76,90,94,104). However, the high acidity and metal concentrations inherent to AMD can inhibit SRB growth (15,88,98), and preferential growth of iron-and sulfur-oxidizing bacteria over SRB has been observed in some treatment wetlands (39).…”

mentioning

confidence: 99%

Diversity of Dissimilatory Sulfite Reductase Genes ( dsrAB ) in a Salt Marsh Impacted by Long-Term Acid Mine Drainage

Moreau

Zierenberg

Banfield

2010

Appl Environ Microbiol

View full text Add to dashboard Cite

Sulfate-reducing bacteria (SRB) play a major role in the coupled biogeochemical cycling of sulfur and chalcophilic metal(loid)s. By implication, they can exert a strong influence on the speciation and mobility of multiple metal(loid) contaminants. In this study, we combined DsrAB gene sequencing and sulfur isotopic profiling to identify the phylogeny and distribution of SRB and to assess their metabolic activity in salt marsh sediments exposed to acid mine drainage (AMD) for over 100 years. Recovered dsrAB sequences from three sites sampled along an AMD flow path indicated the dominance of a single Desulfovibrio species. Other major sequence clades were related most closely to Desulfosarcina, Desulfococcus, Desulfobulbus, and Desulfosporosinus species. The presence of metal sulfides with low ␦ 34 S values relative to ␦ 34 S values of pore water sulfate showed that sediment SRB populations were actively reducing sulfate under ambient conditions (pH of ϳ2), although possibly within less acidic microenvironments. Interestingly, ␦ 34 S values for pore water sulfate were lower than those for sulfate delivered during tidal inundation of marsh sediments. 16S rRNA gene sequence data from sediments and sulfur isotope data confirmed that sulfur-oxidizing bacteria drove the reoxidation of biogenic sulfide coupled to oxygen or nitrate reduction over a timescale of hours. Collectively, these findings imply a highly dynamic microbially mediated cycling of sulfate and sulfide, and thus the speciation and mobility of chalcophilic contaminant metal(loid)s, in AMD-impacted marsh sediments.Salt marshes exhibit high primary production rates (1, 101) and form biogeochemical "transition zones" for nutrient production, transport, and cycling between terrestrial and coastal marine environments (41, 66, 100). These zones also serve to reduce the flux of potentially toxic metals in contaminated groundwater to estuaries (12,99,106). Both functions depend strongly on microbial activity, especially that of sulfate-reducing bacteria (SRB) (42,62,67). SRB recycle much of the sedimentary organic carbon pool in marsh sediments (42-44) and indirectly inhibit production of the greenhouse gas methane (37, 71). They can restrict the mobility of dissolved contaminant metals by inducing precipitation of poorly soluble metal sulfides, and studies have examined their use in constructed wetlands to bioremediate acid mine drainage (AMD) and other metalliferous waste streams (11,35,40,46,50,76,90,94,104). However, the high acidity and metal concentrations inherent to AMD can inhibit SRB growth (15,88,98), and preferential growth of iron-and sulfur-oxidizing bacteria over SRB has been observed in some treatment wetlands (39).For natural salt marshes, 16S ribosomal nucleic acid-and phospholipid fatty acid (PLFA)-based analyses have shown that SRB commonly comprise a significant fraction of the microbial community (13,24,31,34,51,58). Studies of salt marsh dissimilatory sulfite reductase genes (dsrAB), a highly conserved functional phylogenetic marker of pro...

show abstract

mentioning

confidence: 99%

Diversity of Dissimilatory Sulfite Reductase Genes ( dsrAB ) in a Salt Marsh Impacted by Long-Term Acid Mine Drainage

Moreau

Zierenberg

Banfield

2010

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…During the secondary stages of incubation, simple molecules might have been exhausted from the medium while promoting growth of SRB in the primary stages and, consequently, sulphate reduction rates decreased reasonably. The preference of SRB for simple organic molecules is a well reported phenomenon (Nagpal et al 2000;Gibert et al 2004;Tsukamoto et al 2004;Zagury et al 2006). When the SRB started to utilize this complex polymeric substrate, again increments in growth as well as sulphate reductions were recorded (Figs.…”

Section: Resultsmentioning

confidence: 72%

“…It is well known that SRB generally prefer simple low-molecular weight substrates such as sodium lactate and ethanol as energy sources. But being too much expensive, these cannot be used in bioremediation processes at large scales (Postgate 1984;Barnes 1998;El Bayoumy et al 1999;Tsukamoto et al 2004;Huisman et al 2006). However, utilization of various environmental contaminants for instance, halogenated compounds and petroleum hydrocarbons' constituents has been reported by researchers (Fauque et al 1991;Hao et al 1996;Harms et al 1999;Morasch et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Application of sugarcane bagasse for passive anaerobic biotreatment of sulphate rich wastewaters

Hussain

Qazi

2014

Appl Water Sci

View full text Add to dashboard Cite

Biological treatment of sulphate-rich wastewaters employing dissimilatory sulphate reducing bacteria as remedial agents is an attractive technique and has gained importance in the last few years. Industrial effluents enriched with sulphates are generally deficient in electron donors. And thus cannot be treated biologically without supplementation of carbon through an external source. For scalable operations, however, the carbon source must not be expensive. In this context, present study reports the efficiency of biological sulphate reduction using sugarcane bagasse as a cost-effective carbon source. An average 0.00391 ± 0.001 gL -1 day -1 (3.91 mgL -1 day -1) sulphate reduction was observed reaching maximally to 0.00466 ± 0.001 gL -1 day -1 (4.66 mgL -1 day -1) while employing Desulfovibrio fructosovorans-HAQ2 and Desulfovibrio piger-HAQ6 in a 60-day trial of anaerobic incubation using sugarcane bagasse as growth substrate. These findings will be helpful in developing economical bioremediation processes tending to operate for a longer period of time to reduce sulphate contents of contaminated waters.

show abstract

“…In order to thrive, the SRB depend on a community of other bacteria in the system to break down complicated organic materials to simpler carbon molecules that are accessible and easily degradable. Small organic molecules such as lactate, acetate, and ethanol have been shown to be effective as carbon sources in bench-scale bioreactor experiments (Tsukamoto et al, 2004).…”

Section: Sulfate-reducing Bioreactorsmentioning

confidence: 99%

Assessment of Two Field-Scale Sulfate Reducing Bioreactors Using Sulfur Isotopes

Reeder¹,

Branam²,

Olyphant³

2010

JASMR

View full text Add to dashboard Cite

Sulfate-reducing bioreactors (SRBRs) have shown promise as a costeffective option in the passive remediation of acid mine drainage (AMD). While these systems do provide the necessary conditions for increased bacterial activity, little is known about the internal dynamics and the functional lifespan of the systems in field settings. To help address these issues, two field-scale bioreactors are being monitored using an array of sampling ports distributed at varying depths throughout the treatment cells. These internal monitoring ports are located in such a way as to observe 3-D trends in activity occurring within the system. Water samples collected from the ports, as well as samples from the AMD inflow and outflow, have been analyzed for δ 34 S of sulfate as well as standard chemical parameters. Preliminary results indicate that in both systems, bacterial sulfate reduction is occurring yet the degree of reduction is not uniform throughout the cells. Within each system, areas where only a limited amount of bacterial sulfate reduction has occurred are characterized by high concentrations of sulfate coupled with δ 34 S values only slightly different than the influent AMD. In contrast, low sulfate concentrations together with large δ 34 S fractionations are found in areas where extensive bacterial sulfate reduction has taken place. The observed range in fractionation values likely reflects the development of preferential flow paths and points of stagnation within the systems. This implies that not all of the reactive substrate put into a cell will contribute to AMD treatment. The results of this study provide information not typically attainable in smaller laboratory-scale studies and point to the need for further engineering of SRBRs to optimize fieldscale applications.

show abstract

Column experiments for microbiological treatment of acid mine drainage: low-temperature, low-pH and matrix investigations

Cited by 155 publications

References 22 publications

Diversity of Dissimilatory Sulfite Reductase Genes ( dsrAB ) in a Salt Marsh Impacted by Long-Term Acid Mine Drainage

Diversity of Dissimilatory Sulfite Reductase Genes ( dsrAB ) in a Salt Marsh Impacted by Long-Term Acid Mine Drainage

Application of sugarcane bagasse for passive anaerobic biotreatment of sulphate rich wastewaters

Assessment of Two Field-Scale Sulfate Reducing Bioreactors Using Sulfur Isotopes

Contact Info

Product

Resources

About