Column liquid chromatographic analysis of barbiturates in biological fluids

Rop, Pok Phak; Spinazzola, J.; Zahra, A.; Bresson, M.; Quicke, J; Viala, Alain

doi:10.1016/0378-4347(88)80118-x

Cited by 9 publications

(1 citation statement)

References 27 publications

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“…Both amobarbital and secobarbital are metabolized by the liver and they are mostly excreted in urine as their hydroxylated metabolites. Different procedures have been developed in order to determine barbiturates in biological samples, including inmunological techniques (Asselin et al, 1988;O'Connell et al, 1995;Bordes et al, 1997), micellar electrokinetic capillary chromatography (MEKC, Schmutz and Thormann, 1994;Wu et al, 1996;Makino et al, 1997), gas chromatography-mass spectrometry (Mule and Casella, 1989;Stephenson and Tillmans, 1992;Liu et al, 1994;Meathevall, 1997;Namera et al, 1998), and high-performance liquid chromatography (HPLC) using UV detection (Soine and Soine, 1987;Rop et al, 1988;Blevins and Henry, 1995;Kuroda et al, 1996), or mass spectrometry (Kanazawa et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Determination of amobarbital and secobarbital in plasma samples using micellar liquid chromatography

Quiñones-Torrelo

Martı́n-Biosca

Sagrado

et al. 2000

Biomed. Chromatogr.

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A new liquid chromatographic procedure for the determination of amobarbital and secobarbital in plasma samples is proposed. The method uses a Spherisorb octadecylsilane ODS-2 C(18) analytical column, a guard column of similar characteristics and 0.04 M CTAB solution buffered at pH 7.5 containing 3% 1-propanol as micellar mobile phase. The UV detection was carried out at 250 nm. Butabarbital was used as internal standard. Plasma samples preparation only required adequate dilution with the mobile phase before injection into the chromatographic system. The limits of detection were 0.2 and 0.4 mg/L for amobarbital and secobarbital, respectively. The proposed method allows the determination of amobarbital and secobarbital in plasma at therapeutic levels.

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