Column Liquid Chromatography:  Equipment and Instrumentation

Abstract

Immunochemical-Based 48R Other Detectors 48R Computation 48R Data Reduction 48R System Evaluation and Validation 48R Literature Cited 48RThis review covers fundamental developments in column liquid chromatography (LC) equipment and instrumentation for the period of October 1997 through October 1999. As with past issues, separate reviews on theory and methodology, size exclusion chromatography (SEC), and capillary electrophoresis (CE) are included in this volume of Fundamental Reviews. Capillary electrochromatography (CEC) is reviewed as part of the CE section. Because of the close relationship between capillary LC and electrophoretic techniques, references with technology pertinent to both were included. In addition, high-performance liquid chromatography (HPLC) and LC are considered synonymous. Although this review was not intended to cover applications, applications that contained developments and improvements of LC equipment and instrumentation or were used to demonstrate equipment performance were also included.The database for the majority of this review was Chemical Abstracts. This paper was not intended to be a comprehensive review of all published papers during this time period, but of those papers that were considered to be significant developments or improvements. This review has also largely restricted the covered material to the English language literature. Although the vast majority of articles were from peer-reviewed publications, included are relevant articles from widely read periodicals such as LC-GC and American Laboratory. INSTRUMENTATIONReviews. Fundamental developments in column LC equipment and instrumentation for the period of October 1995 through October 1997 were reviewed by LaCourse and Dasenbrock (A1). This paper is a continuation of that effort. Kennedy et al. (A2) reviewed fast analytical-scale separations by CE and LC. Raulin et al. (A3) discussed the use of chromatographic instrumentation in space exploration for exobiological studies. A wide range of techniques were reviewed. They noted that there is now a strong need for new chromatographic instrumentation (e.g., HPLC) in space, in particular to perform detailed molecular analyses of complex nonvolatile organics, including macromolecular compounds. Serwe et al. (A4) limited their review of HPLC to protein and peptide analysis. In addition to theory, practical aspects such

Studies on the distribution and radioimmunoimaging of 99mTc-labeled 5-fluorouracil loaded immunological nanoparticles in tissues and human gastric carcinoma xenografts

“…With time, the 5-FU tumor concentration gradually increased. It has been confirmed that employment of 5-FU for treating gastrointestinal cancer is a rational therapy, and is consistent with the 5-FU pharmacokinetics [8] .…”

Guest Editorial

“…Biennial review; fundamental developments in column liquid chromatography equipment and instrumentation from October 1997 to October 1999; instrumentation, columns, and a variety of detectors are covered (LaCourse, 2000). Biennial review; developments in equipment and instrumentation from October 1995through October 1997(LaCourse and Dasenbrock, 1998.…”

Preparation of high‐selective HPLC packing materials based on alternating copolymer‐grafted silica

“…At present, HPLC is one of the most widely used analytical methods because of its good separation power and wide applicability [6][7][8]. Most progress in RP-HPLC separation has been achieved as a result of the introduction of various silicabased stationary phase materials.…”