Column-switching high-performance liquid chromatographic method for the determination of zaltoprofen in rat plasma

Choi, Sun Ok; Um, So Young; Jung, Sung Hee; Jung, Seo Jeong; Kim, Joo Il; Lee, Hwa Jeong; Chung, Soo Youn

doi:10.1016/j.jchromb.2005.11.003

Cited by 16 publications

(14 citation statements)

References 21 publications

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“…The devised method was found to be selective and reliable, and faster and more straightforward than other reported methods. 8,9 The authors also applied the developed method to a pharmacokinetic study in 24 healthy male Korean volunteers administered zaltoprofen tablets (160 mg).…”

mentioning

confidence: 99%

Determination of zaltoprofen in human plasma by liquid chromatography with electrospray tandem mass spectrometry: application to a pharmacokinetic study

Lee

Seo

Kim

et al. 2006

Rapid Comm Mass Spectrometry

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In the present study, we developed a method coupling liquid-liquid extraction (LLE) to high-performance liquid chromatography (HPLC) with positive ion electrospray ionization tandem mass spectrometry (ESI-MS/MS) to determine zaltoprofen levels in human plasma, using enalapril as internal standard (IS). The high sensitivity and specificity of MS/MS detection enabled the use of small plasma volumes (250 microL) and a simple LLE procedure. Furthermore, the short run-times (2 min) involved are compatible with the requirements of large-scale clinical studies. Ion acquisition was performed in multiple reaction monitoring (MRM) mode by monitoring the transitions m/z 299.3 > 225.0 for zaltoprofen and m/z 377.4 > 234.2 for the IS enalapril. The limit of detection (LOD) was 0.01 microg/mL and the lower limit of quantitation (LLOQ) was 0.05 microg/mL. The devised method was linear over the studied range (0.05-20 microg/mL), with r2 > 0.99 and a run-time of 2 min. Intra-day precisions fell in the range 2.0-13.8%, inter-day precisions in the range 2.1-3.9%, and intra- and inter-day accuracies in the range 102.8-114.1%. The described method provides a fast and sensitive analytical tool for zaltoprofen and was successfully applied to a 24-subject pharmacokinetic study.

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mentioning

confidence: 99%

Determination of zaltoprofen in human plasma by liquid chromatography with electrospray tandem mass spectrometry: application to a pharmacokinetic study

Lee

Seo

Kim

et al. 2006

Rapid Comm Mass Spectrometry

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“…Although earlier publications have described column-switching high-performance liquid chromatography/ultraviolet (HPLC-UV) method [10,11] and a liquid chromatography with electrospray tandem mass spectrometry method [12] for the analysis of zaltoprofen in biological samples, at present, there are few reports about the direct determination of zaltoprofen glucuronide formed by in vitro or in vivo assay since it is very difficult to obtain the standards of the glucuronides. Previously reported the glucuronidation of ZPF was measured through the disappearance of ZPF or as patent drug equivalents after enzymatic hydrolysis [7], which was obviously lack of precise and very tedious.…”

Section: Zaltoprofenmentioning

confidence: 99%

Biosynthesis and stereoselective analysis of (−)- and (+)-zaltoprofen glucuronide in rat hepatic microsomes and its application to the kinetic analysis

Wang

Zeng

2011

Journal of Chromatography B

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“…Liquid -liquid extraction (LLE) and SPE are general procedures to extract analytes from a biological matrix. However, those procedures are laborious and time consum-ing [11,12]. Nowadays, there is increasing demand for automation and high-throughput analysis, and online sample preparation procedures are increasingly used [13].…”

Section: Introductionmentioning

confidence: 99%

“…Especially, column-switching systems via a switching valve have great advantages in permitting the direct injection of protein rich samples, such as the serum [11, 12, 14 -16]. To minimize the possible introduction of analytical artifacts through excessive sample manipulation, a new analytical method has been developed and validated in our laboratory using HPLC with columnswitching [11,12,14,16,17]. The column-switching technique avoids offline procedures for sample cleanup [11,12,14,15,18].…”

Section: Introductionmentioning

confidence: 99%

“…To minimize the possible introduction of analytical artifacts through excessive sample manipulation, a new analytical method has been developed and validated in our laboratory using HPLC with columnswitching [11,12,14,16,17]. The column-switching technique avoids offline procedures for sample cleanup [11,12,14,15,18]. This method allowed serum samples to be directly injected onto a fully automated HPLC system, thereby resulting in a simple, convenient, and accurate assay even without an internal standard.…”

Section: Introductionmentioning

confidence: 99%

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Determination of the active metabolites of sibutramine in rat serum using column‐switching HPLC

Um¹,

Kim²,

Kim³

et al. 2008

J of Separation Science

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A simple and direct analysis using column-switching HPLC method was developed and validated for the quantification of active metabolites of sibutramine, N-mono-desmethyl metabolite (metabolite 1, M1) and N-di-desmethyl metabolite (metabolite 2, M2) in the serum of rats administered sibutramine HCl (5.0 mg/kg, p.o.). Rat serum was directly injected onto the precolumn without sample prepreparation step following dilution with mobile phase A, i. e., methanol-ACN-20 mM ammonium phosphate buffer (pH 6.0 with phosphoric acid) (8.3:4.5:87.2 by volume). After the endogenous serum components were eluted to waste, the system was switched and the analytes were eluted to the trap column. Active metabolites M1 and M2 were then back-flushed to the analytical column for separation with mobile phase B, i. e., methanol-ACN-20 mM ammonium phosphate buffer (pH 6.0 with phosphoric acid) (35.8:19.2:45 by volume) and detected at 223 nm. The calibration curves of active metabolites M1 and M2 were linear in the range of 0.1-1.0 microg/mL and 0.15-1.8 microg/mL. This method was fully validated and shown to be specific, accurate (10.4-10.7% error), and precise (1.97-8.79% CV). This simple and rapid analytical method using column-switching appears to be useful for the pharmacokinetic study of active metabolites (M1 and M2) of sibutramine.

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Column-switching high-performance liquid chromatographic method for the determination of zaltoprofen in rat plasma

Cited by 16 publications

References 21 publications

Determination of zaltoprofen in human plasma by liquid chromatography with electrospray tandem mass spectrometry: application to a pharmacokinetic study

Determination of zaltoprofen in human plasma by liquid chromatography with electrospray tandem mass spectrometry: application to a pharmacokinetic study

Biosynthesis and stereoselective analysis of (−)- and (+)-zaltoprofen glucuronide in rat hepatic microsomes and its application to the kinetic analysis

Determination of the active metabolites of sibutramine in rat serum using column‐switching HPLC

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