Column-switching HPLC–MS/MS analysis of ropivacaine in serum, ultrafiltrate and drainage blood for validating the safety of blood reinfusion

Breindahl, Torben; Simonsen, Ole; Andreasen, Kirsten

doi:10.1016/j.jchromb.2009.11.028

Cited by 21 publications

(14 citation statements)

References 28 publications

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“…Serum samples were analysed for total and free ropivacaine concentrations by High-Performance Liquid Chromatography (HPLC) with tandem mass spectrometry [7]. Determination of free ropivacaine was performed after ultrafiltration on fresh serum.…”

Section: Methodsmentioning

confidence: 99%

Autologous Blood Transfusion after Local Infiltration Analgesia with Ropivacaine in Total Knee and Hip Arthroplasty

Breindahl

Simonsen

Hindersson

et al. 2012

Anesthesiology Research and Practice

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Aims. To study the safety of autotransfusion following local infiltration analgesia (LIA) with ropivacaine. Background. Knowledge of blood concentrations of ropivacaine after LIA and autotransfusion is crucial. However, very limited data are available for toxicological risk assessment. Methods. Autotransfusion was studied in patients after total knee arthroplasty (TKA: n = 25) and total hip arthroplasty (THA: n = 27) with LIA using 200 mg ropivacaine, supplemented with two postoperative bolus injections (150 mg ropivacaine). Drainage blood was reinfused within 6 h postoperatively. Results. Reinfusion caused a significant increase in the serum concentration of total ropivacaine for TKA from 0.54 ± 0.17 (mean ± SD) to 0.79 ± 0.20 μg/mL (P < 0.001) and a nonsignificant increase for THA from 0.62 ± 0.17 to 0.63 ± 0.18 μg/mL. The maximum free (unbound) concentration after reinfusion was 0.038 μg/mL. Peak total and free venous ropivacaine concentrations after 8 h and 16 h postoperative bolus injections were 2.6 μg/mL and 0.11 μg/mL, respectively. All concentrations observed were below the threshold for toxicity and no side effects were observed. Conclusion. Autotransfusion of patients undergoing knee or hip arthroplasty after local infiltration analgesia with 200 mg ropivacaine can be performed safely, even supplemented with 8 h and 16 h postoperative bolus injections.

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Section: Methodsmentioning

confidence: 99%

Autologous Blood Transfusion after Local Infiltration Analgesia with Ropivacaine in Total Knee and Hip Arthroplasty

Breindahl

Simonsen

Hindersson

et al. 2012

Anesthesiology Research and Practice

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“…Only six small studies have been published about (possible) reinfusion following total knee arthroplasty (TKA) with LIA using ropivacaine [8,9,10,11,12,13]. Hitherto, reinfusion was studied in two studies with different doses (200 mg vs. 150 mg), additional bolus injections (twice vs. none) and even other comparators such as tourniquet usage and hip and knee arthroplasty [9,10].…”

Section: Introductionmentioning

confidence: 99%

Safety of blood reinfusion after local infiltration analgesia with ropivacaine in total knee arthroplasty

Thomassen¹,

Touw²,

Woude³

et al. 2014

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“…2 The concentration of ropivacaine was previously measured by several ways. [3][4][5] Liquid chromatography-mass spectroscopy (LC-MS) is used to quantify ropivacaine in human plasma; 3 its retention time is 8.9 min and limit of quantification was 1.0 mg/L. An effective method using high performance-MS/MS (HPLC-MS/MS) was developed to assess ropivacaine concentrations, 4 yielding a ropivacaine retention time of 6.0 min and quantification limit of 0.01 mg/L.…”

Section: Introductionmentioning

confidence: 99%

“…[3][4][5] Liquid chromatography-mass spectroscopy (LC-MS) is used to quantify ropivacaine in human plasma; 3 its retention time is 8.9 min and limit of quantification was 1.0 mg/L. An effective method using high performance-MS/MS (HPLC-MS/MS) was developed to assess ropivacaine concentrations, 4 yielding a ropivacaine retention time of 6.0 min and quantification limit of 0.01 mg/L. A method of simultaneously analyzing the concentrations of various local anesthetics (procaine, lidocaine, ropivacaine, tetracaine, and bupivacaine in human plasma) yielded a ropivacaine retention time of 6.3 min and a quantification limit of 50 mg/L.…”

Section: Introductionmentioning

confidence: 99%

Online Detection of Ropivacaine in Drip Bags Using Polypyrrole/graphene Oxide Materials

Chen

Luk

et al. 2014

J Chinese Chemical Soc

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A novel method of measuring the concentration of the local anesthetic ropivacaine drip bags used in hospitals was developed using electrochemical impedance technique. Polypyrrole (Ppy)/graphene oxide (GO) composites were prepared by electrochemically polymerization over Au electrodes, which served as working electrodes. The Ppy/GO composite electrodes were characterized using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Various concentrations of ropivacaine (0.1-20 ppm) were prepared in a sample drip bag solution of 0.9% NaCl from hostpital. The composite Ppy/1% GO electrode exhibited strongest linearity (R 2 = 0.960) than the Ppy electrode (R 2 = 0.928) at a frequency of 100 kHz. The detection limits of ropivacaine on Ppy and Ppy/1% GO were calculated as 0.11 ppm and 0.08 ppm, respectively. The proposed system yielded response and recovery times under 1 sec detecting 0.1 ppm (100 mg/L) of ropivacaine; thus, the proposed online method is promising for measuring ropivacaine concentrations in drip bags. Molecular simulations and equivalent circuits were applied to explain the dynamic behavior of ropivacaine detection system using Ppy and Ppy/GO materials.

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Column-switching HPLC–MS/MS analysis of ropivacaine in serum, ultrafiltrate and drainage blood for validating the safety of blood reinfusion

Cited by 21 publications

References 28 publications

Autologous Blood Transfusion after Local Infiltration Analgesia with Ropivacaine in Total Knee and Hip Arthroplasty

Autologous Blood Transfusion after Local Infiltration Analgesia with Ropivacaine in Total Knee and Hip Arthroplasty

Safety of blood reinfusion after local infiltration analgesia with ropivacaine in total knee arthroplasty

Online Detection of Ropivacaine in Drip Bags Using Polypyrrole/graphene Oxide Materials

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