Comammox bacteria predominate among ammonia-oxidizing microorganisms in municipal but not in refinery wastewater treatment plants

He, Shishi; Zhao, Zijian; Tian, Zhichao; Chi, Xu; Liu, Yuan; Zhang, Yinghui; Zheng, Maosheng

doi:10.1016/j.jenvman.2022.115271

Cited by 15 publications

(14 citation statements)

References 50 publications

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“…Pan et al (2016) validated a model based on the hypothesis that AOA have higher NH 4 + affinities (0.06 g N/m −3 ) than AOB (2.4 g N/m −3 ) so that AOA are only competitive with AOB in low NH 4 + environments. This link between NH 4 + concentration and AOA abundance is also supported by results obtained by He et al (2022) with refinery wastewater and Sun et al (2022) with “high influent strength” swine wastewater (COD, 516.94 ± 4.18 mg/L; NH 4 + , 100.65 ± 2.65) in a constructed wetland, where the calculated AOB to AOA abundance ratio was 51.6. Based on the results of previous studies, it was hypothesized that AOA would not be abundant in the SA WWTPs as the influent NH 4 + concentrations was high in the WWTPs from both geographical locations (Table 2).…”

Section: Resultssupporting

confidence: 81%

“…In a similar study, He et al (2022) found that Nitrosomonas usually dominated over the second most prevalent genus, Nitrosospira , in eight AS WWTPs in China treating domestic effluent. Nitrosomonas oligotropha was the most abundant species, ostensibly because of its high affinity for NH 3 /NH 4 + .…”

Section: Resultsmentioning

confidence: 79%

“…Yu and Zhang (2012) failed to detect AOA from an AS WWTP in Hong Kong using metagenomic and metatranscriptomic approaches to evaluate the microbial community structure and gene expression. In eight WWTPs in China treating municipal wastewater, He et al (2022) found that AOB were…”

Section: Ammonium Oxidizing Bacterial Composition In the Study Wastew...mentioning

confidence: 99%

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Ammonium oxidizing bacterial populations in South African activated sludge wastewater treatment plants

Welz,

Thobejane,

van Blerk

2023

Water Environment Research

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This is the first study that describes ammonium oxidizing bacterial populations and correlations of these populations with a range of criteria in activated sludge wastewater treatment plants in South Africa. In this study, not only the influent, but also the activated sludge chemistry was comprehensively characterized. Multivariate statistical analyses were used to determine the relative significances of the geographical location (factor: site), wastewater treatment plant process (factor: configuration), seasonality (factor: season), and environmental parameters on the ammonium oxidizing bacterial genera in six municipal activated sludge wastewater treatments plants from two sites (the Cities of Cape Town and Ekurhuleni).The geographical location (site) was significant for selection of the ammonium oxidizing genera (Global ANOSIM R value=0.538, p=0.001). It was established that the inter‐site differences were not climatic in origin, nor related to the composition of the influent, but were rather driven by the activated sludge chemistry. It was found using BEST analysis that the activated sludge ammonia, activated sludge total phosphate and activated sludge total chemical oxygen demand were the most significant (p<0.001) drivers for ammonium oxidizing bacterial selection (ANOSIM Global R value 0.419), and were significantly higher in the activated sludge from the City of Cape Town wastewater treatment plants. Nitrosospira was the most abundant ammonium oxidizing bacterial genus, with notably higher relative and estimated actual abundances in the City of Cape Town wastewater treatment plants than the City of Ekurhuleni wastewater treatment plants. The strong selection of Nitrosospira in the City of Cape Town wastewater treatment plants with higher nutrient concentrations strongly suggests that high concentrations of activated sludge ammonia, activated sludge total phosphate and activated sludge total chemical oxygen demand are key selective drivers for this genus.

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Section: Resultssupporting

confidence: 81%

Section: Resultsmentioning

confidence: 79%

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Ammonium oxidizing bacterial populations in South African activated sludge wastewater treatment plants

Welz,

Thobejane,

van Blerk

2023

Water Environment Research

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“…Since then, lineage II Nitrospira comammox species have been widely detected in forest and agricultural soils, freshwater and brackish sediments, and waste and drinking water treatment plants ( Pinto et al, 2016 ; Camejo et al, 2017 ; Pjevac et al, 2017 ; Fowler et al, 2018 ; Xia et al, 2018 ; Spasov et al, 2020 ; Yang et al, 2020 ). Comammox species were shown to be active participants and to even dominate some wastewater systems ( Roots et al, 2019 ; He et al, 2022 ). Despite its widespread occurrence, major environmental variables governing the distribution and abundance of comammox and hence their role in microbial ecosystems are not well characterized.…”

Section: Introductionmentioning

confidence: 99%

Photoinhibition of comammox reaction in Nitrospira inopinata in a dose- and wavelength-dependent manner

Gottshall

Godfrey²,

Li³

et al. 2022

Front. Microbiol.

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Apparent contribution of complete ammonia-oxidizing organisms (comammox) to the global nitrogen cycle highlights the necessity for understanding niche differentiation of comammox bacteria among other ammonia oxidizers. While the high affinity for ammonia of the comammox species Nitrospira inopinata suggests their niche partitioning is expected to be centered in oligotrophic environments, their absence in nutrient-depleted environments (such as the oceans) suggests that other (abiotic) factors might control their distribution and spatial localization within microbial communities. Many ammonia- and nitrite-oxidizing organisms are sensitive to light; however, the photosensitivity of comammox has not been explored. Since comammox bacteria encode enzymatic machinery homologous to canonical ammonia-and nitrite-oxidizers, we hypothesized that comammox N. inopinata, the only available pure culture of this group of microorganisms, may be inhibited by illumination in a similar manner. We evaluated the impact of light intensity, wavelength, and duration on the degree of photoinhibition for cultures of the comammox species N. inopinata and the soil ammonia-oxidizing archaea Nitrososphaera viennensis. Both species were highly sensitive to light. Interestingly, mimicking diurnal light exposure caused an uncoupling of ammonia and nitrite oxidation in N. inopinata, indicating nitrite oxidation might be more sensitive to light exposure than ammonia oxidation. It is likely that light influences comammox spatial distribution in natural environments such as surface fresh waters according to diurnal cycles, light attenuation coefficients, and the light penetration depths. Our findings therefore provide ecophysiological insights for further studies on comammox both in field and laboratory settings.

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“…Additionally, comammox are dominant ammonia oxidizers in WWTPs with relatively long solid retention times because of their slow growth rate [ 28 , 29 , 30 ]. Wastewater resources affect the distribution of comammox, which are more abundant in municipal wastewater than in refinery wastewater [ 31 ]. Furthermore, some researchers found that comammox are the dominant nitrifiers under limited DO concentrations (<1 mg/L), whereas traditional nitrification in WWTPs requires relatively high DO concentrations (>2 mg/L) [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

Abundance and Niche Differentiation of Comammox in the Sludges of Wastewater Treatment Plants That Use the Anaerobic–Anoxic–Aerobic Process

Zhang

Wang

et al. 2022

Life

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Complete ammonia oxidizers (comammox), which directly oxidize ammonia to nitrate, were recently identified and found to be ubiquitous in artificial systems. Research on the abundance and niche differentiation of comammox in the sludges of wastewater treatment plants (WWTPs) would be useful for improving the nitrogen removal efficiency of WWTPs. Here, we investigated the relative abundance and diversity of comammox in fifteen sludges of five WWTPs that use the anaerobic–anoxic–aerobic process in Jinan, China, via quantitative polymerase chain reaction and high-throughput sequencing of the 16S rRNA gene and ammonia monooxygenase gene. In the activated sludges in the WWTPs, comammox clade A.1 was widely distributed and mostly comprised Candidatus Nitrospira nitrosa-like comammox (>98% of all comammox). The proportion of this clade was negatively correlated (p < 0.01) with the dissolved oxygen (DO) level (1.7–8 mg/L), and slight pH changes (7.20–7.70) affected the structure of the comammox populations. Nitrospira lineage I frequently coexisted with Nitrosomonas, which generally had a significant positive correlation (p < 0.05) with the DO level. Our study provided an insight into the structure of comammox and other nitrifier populations in WWTPs that use the anaerobic–anoxic–aerobic process, broadening the knowledge about the effects of DO on comammox and other nitrifiers.

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Comammox bacteria predominate among ammonia-oxidizing microorganisms in municipal but not in refinery wastewater treatment plants

Cited by 15 publications

References 50 publications

Ammonium oxidizing bacterial populations in South African activated sludge wastewater treatment plants

Ammonium oxidizing bacterial populations in South African activated sludge wastewater treatment plants

Photoinhibition of comammox reaction in Nitrospira inopinata in a dose- and wavelength-dependent manner

Abundance and Niche Differentiation of Comammox in the Sludges of Wastewater Treatment Plants That Use the Anaerobic–Anoxic–Aerobic Process

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