Comano's (Trentino) thermal water interferes with the expression and secretion of vascular endothelial growth factor-A protein isoforms by cultured human psoriatic keratinocytes: A potential mechanism of its anti-psoriatic action

Chiarini, Anna Maria; Prà, Ilaria Pierpaola Dal; Pacchiana, Raffaella; Menapace, Lia; Zumiani, Giuseppe; Zanoni, Mauro; Armato, Ubaldo

doi:10.3892/ijmm.18.1.17

Cited by 19 publications

(25 citation statements)

References 51 publications

(74 reference statements)

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“…Here, we will show that the addition of CW (in total or partial stead of DW) to the growth medium remarkably hinders the hyper-expression of TNF-· and the heightened production and release of IL-8 on the part of the psoriatic keratinocytes. Therefore, our previous (49,50) and present findings are consistent with CW being endowed with a complex phenotype-and cytokine/chemokine-regulating potential that translates into valuable anti-psoriatic therapeutic benefits.…”

Section: Introductionsupporting

confidence: 73%

“…In conclusion, our previous (49,50) and present observations support the view that CW balneotherapy may elicit its beneficial effects by interfering with an improper local production and secretion of several chemokines and cytokines, including TNF-·, IL-8, IL-6, and various VEGF-A isoforms, and by attenuating some facets, such as CK-16 expression, of the psoriatic phenotype, which altogether locally underlie the epidermal hyperplasia and dermal neoangiogenesis, inflammation, and leukocyte infiltration phenomena proper of psoriatic illness.…”

Section: Discussionmentioning

confidence: 93%

“…In previous studies (49,50), we showed that exposure to CW interferes with IL-6, VEGF-A isoform, and CK-16 expression by human psoriatic keratinocytes. To further clarify the mechanisms involved in the therapeutic effectiveness of CW balneotherapy in psoriasis, in this study we investigated CW's effects on TNF-· and IL-8 production and secretion by epidermal keratinocytes isolated from lesional skin biopsies and kept in confluent cultures.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comano's (Trentino) thermal water interferes with tumour necrosis factor-α expression and interleukin-8 production and secretion by cultured human psoriatic keratinocytes: Yet other mechanisms of its anti-psoriatic action

Prà

Chiarini²,

Pacchiana³

et al. 2007

Int J Mol Med

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Abstract. Thermal balneotherapy with Comano's spa water (CW; Trentino, Italy) is beneficial for psoriasis and other skin disorders but its operative mechanisms are largely unknown. Previously, we showed that CW interferes with the production and secretion of IL-6 and various VEGF-A isoforms and with CK-16 expression by cultured human psoriatic keratinocytes. In this study, confluent cultures of epidermal keratinocytes isolated from the lesional areas of 9 psoriatic patients were exposed for 11-13 days to DMEM, whose chemicals had been dissolved in either deionised water (DW-DMEM, controls) or CW (CW-DMEM, treated cells), in order to assess the expression and secretion of TNF-· and IL-8 by such cells. The results gained by means of immunocytochemistry, Western immunoblotting (WB), and ELISA assays showed that CW exposure significantly downregulated the intracellular levels of TNF-·, a key inducer of IL-8, IL-6, and other chemokines. However, no assayable TNF-· secretion occurred in keratinocyte-conditioned DWand CW-DMEM samples. Moreover, the intracellular levels and secretion rates of IL-8 were also markedly reduced in the protein extracts and conditioned media of CW-DMEMincubated keratinocytes. Notably, the most effective inhibition of IL-8 secretion was elicited by a 25% CW fraction in the DMEM. Altogether, our findings indicate that by attenuating at lesional skin sites the deregulated production and secretion of a cascade of several cytokines and chemokines (e.g. TNF-·, IL-8, IL-6, and various VEGF-A isoforms), and by offsetting the keratinocytes' abnormal differentiation program entailing CK-16 expression, CW balneotherapy may beneficially influence the clinical manifestations of psoriasis.

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Section: Introductionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comano's (Trentino) thermal water interferes with tumour necrosis factor-α expression and interleukin-8 production and secretion by cultured human psoriatic keratinocytes: Yet other mechanisms of its anti-psoriatic action

Prà

Chiarini²,

Pacchiana³

et al. 2007

Int J Mol Med

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“…Actually, some studies have shown that Comano thermal water is able to reduce the expression of some cytokines (TNF-α, IL-6 and IL-8) involved in the inflammatory process, relating this property to its anti-psoriatic efficacy in humans (15,16). Unlike corticosteroid action, such a specific effect against the inflammatory reaction does not counteract cell proliferation.…”

Section: Discussionmentioning

confidence: 99%

Effects of thermal water on skin regeneration

et al. 2012

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Abstract. An experimental study was carried out in an animal (New Zealand white rabbit) wound model to evaluate any effects of a hypotonic, bicarbonate-calcium-magnesium mineral water (Comano thermal water) on skin regeneration, comparing the healing rate of split-thickness skin graft donor sites treated with the thermal water wet dressing versus a standard petrolatum gauze dressing versus a saline solution wet dressing. The study was performed in two steps; an overall of 22 animals were enrolled in the study. The wound healing progress was evaluated both by the surgeons and by the histologists. Sixty-four punch biopsies were examined in all. The histological samples were examined after staining with haematoxylin and eosin, Masson's and orcein staining and under a transmission electron microscope. The data were statistically analysed. The Comano thermal water proved to improve skin regeneration, not only by increasing keratinocyte proliferation and migration but also favourably modulating the regenerated collagen and elastic fibres in the dermis. We propose that the results of the topical treatment with the thermal water could be due to the favourable combination of a local wet environment with an anti-inflammatory action and that the regenerative properties of Comano thermal water observed in rabbits could also be applied for human use.

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“…However, most of the mechanisms through which the clinical signs of psoriasis (and of the other above mentioned skin disorders) are improved by means of CW balneotherapy have not as yet been clarified. In a previous study, we showed that exposure to CW interferes with VEGF-A isoform expression and secretion by the human psoriatic keratinocytes (54). To further clarify the mechanism(s) possibly involved in the therapeutic effectiveness of CW balneotherapy in psoriasis, we investigated CW's effects on IL-6 hyper-production and hyper-secretion and on CK-16 expression by epidermal keratinocytes isolated from lesional skin biopsies.…”

Section: Introductionmentioning

confidence: 99%

Comano's (Trentino) thermal water interferes with interleukin-6 production and secretion and with cytokeratin-16 expression by cultured human psoriatic keratinocytes: Further potential mechanisms of its anti-psoriatic action

Chiarini

Prà²,

Pacchiana³

et al. 2006

Int J Mol Med

Self Cite

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Abstract. Thermal balneotherapy with Comano's spa water (CW; Trentino, Italy) is used for psoriasis and other skin disorders but its mechanisms of action are mostly unknown. Previously, we showed that CW can interfere with the expression and secretion of various VEGF-A isoforms by cultured human psoriatic epidermal keratinocytes. In this study, confluent cultures of IL-6-hypersecreting keratinocytes isolated from 6 psoriatic patients were exposed for 11-15 days to DMEM, the chemicals of which had been dissolved in either deionised water (DW-DMEM, controls) or CW (CW-DMEM, treated cells). As detected by means of immunocytochemistry, Western immunoblotting, and ELISA assays, the intracellular levels and secretion rates of IL-6 were drastically curtailed in the CW-DMEM-incubated keratinocytes and in their cell-conditioned media. A nearly maximal inhibition of IL-6 release had already been induced by a CW fraction in the DMEM as low as 25%. CW exposure also promptly, intensely, and persistently down-regulated the expression of cytokeratin-16 (CK-16), a marker associated with keratinocyte psoriatic phenotype. Hence, CW balneotherapy may beneficially affect the clinical manifestations of psoriasis via an attenuation of the local deregulation of several cytokines/chemokines, including IL-6 and VEGF-A isoforms, and of a concurrent, abnormal cell differentiation program entailing the expression, amongst other proteins, of CK-16.

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Comano's (Trentino) thermal water interferes with the expression and secretion of vascular endothelial growth factor-A protein isoforms by cultured human psoriatic keratinocytes: A potential mechanism of its anti-psoriatic action

Abstract: Abstract. Thermal balneotherapy with Comano spa's water (CW; Trentino, Italy) is used for psoriasis and other skin disorders but the mechanism(s) of action of this hypotonic water are unknown.

Cited by 19 publications

References 51 publications

Comano's (Trentino) thermal water interferes with tumour necrosis factor-α expression and interleukin-8 production and secretion by cultured human psoriatic keratinocytes: Yet other mechanisms of its anti-psoriatic action

Comano's (Trentino) thermal water interferes with tumour necrosis factor-α expression and interleukin-8 production and secretion by cultured human psoriatic keratinocytes: Yet other mechanisms of its anti-psoriatic action

Effects of thermal water on skin regeneration

Comano's (Trentino) thermal water interferes with interleukin-6 production and secretion and with cytokeratin-16 expression by cultured human psoriatic keratinocytes: Further potential mechanisms of its anti-psoriatic action

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