1960
DOI: 10.1038/188428a0
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Comaprison of Strains of Streptomyces griseus which produce Streptomycin and those which do not

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Cited by 14 publications
(10 citation statements)
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“…Cloning in streptomycetes was done in Streptomyces lividans TK64 [7], S. griseus 45H [8] and S. griseus 52‐1 [8], using the cloning vector pIJ702 [7]. For selection of the plasmid, 50 or 5 μg ml −1 thiostrepton was used in solid and in liquid medium, respectively.…”
Section: Methodsmentioning
confidence: 99%
“…Cloning in streptomycetes was done in Streptomyces lividans TK64 [7], S. griseus 45H [8] and S. griseus 52‐1 [8], using the cloning vector pIJ702 [7]. For selection of the plasmid, 50 or 5 μg ml −1 thiostrepton was used in solid and in liquid medium, respectively.…”
Section: Methodsmentioning
confidence: 99%
“…Strains and cultivation: Strains used in these experiments were described earlier (SZAB~ et al 1961, VITLLIS et al 1963. Streptomyces griseus No.…”
Section: Methodsmentioning
confidence: 99%
“…When required for the maintenance of the plasmids, ampicillin (100 Wg ml 31 ) and kanamycin (25 Wg ml 31 ) were added. Cloning in streptomycetes was done in Streptomyces lividans TK64 [7], S. griseus 45H [8] and S. griseus 52-1 [8], using the cloning vector pIJ702 [7]. For selection of the plasmid, 50 or 5 Wg ml 31 thiostrepton was used in solid and in liquid medium, respectively.…”
Section: Materials and Strainsmentioning
confidence: 99%