Combating planktonic and biofilm growth of Serratia marcescens by repurposing ebselen

Shaikh, Shaukat Ali M.; Patel, Bharti; Priyadarsini, Indira K.; Vavilala, Sirisha L.

doi:10.1007/s10123-022-00301-5

Cited by 5 publications

(4 citation statements)

References 42 publications

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“…The fluorescence was measured spectrophotometrically at excitation 485 nm and emission at 530 nm for every hour till 5 h. Bacterial cells without Ts-AgNPs treatment act as a control. The graph was plotted as concentration on X-axis and ROS production on Y-axis [58].…”

Section: Intracellular Reactive Oxygen Species (Ros) Assaymentioning

confidence: 99%

Exploring the efficacy of tryptone-stabilized silver nanoparticles against respiratory tract infection-causing bacteria: a study on planktonic and biofilm forms

Pandey,

Pradhan,

Meher

et al. 2024

Biomed. Mater.

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Respiratory tract infections are a common cause of mortality and morbidity in the human population. The overuse of antibiotics to overcome such infections has led to antibiotic resistance. The emergence of Multidrug resistant (MDR) bacteria is necessitating the development of novel therapeutic techniques in order to avoid a major global clinical threat. Our study aims to investigate the potential of Tryptone stabilised silver nanoparticles (Ts-AgNPs) on planktonic and biofilms produced by Klebsiella pneumoniae and Pseudomonas aeruginosa. The MIC50 of Ts-AgNPs was found to be as low as 1.7 μg/mL and 2.7 μg/mL for K. pneumoniae and P.aeruginosa respectively. Ts-AgNPs ability to alter redox environment by producing intracellular ROS, time-kill curves showing substantial decrease in the bacterial growth and significantly reduced colony forming units further validate its antimicrobial effect. The biofilm inhibition and eradication ability of Ts-AgNPs was found to be as high as 93% and 97% in both the tested organisms. A significant decrease in the eDNA and EPS quantity in Ts-AgNPs treated cells proved its ability to successfully distort the matrix and matured biofilms. Interestingly Ts-AgNPs also attenuated QS-induced virulence factors production,. This study paves way to develop Ts-AgNPs as novel antibiotics against respiratory tract infections causing bacterial biofilms.

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Section: Intracellular Reactive Oxygen Species (Ros) Assaymentioning

confidence: 99%

Exploring the efficacy of tryptone-stabilized silver nanoparticles against respiratory tract infection-causing bacteria: a study on planktonic and biofilm forms

Pandey,

Pradhan,

Meher

et al. 2024

Biomed. Mater.

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“…Ebselen also exhibits an impact on bioflms produced by Gram-negative bacteria, although the underlying molecular mechanisms remain incompletely understood. Utilizing the crystal violet assay in microtiter plates, Shaikh [67] demonstrated signifcant inhibition of bioflm formation on Neisseria mucosa by ebselen. Moreover, the bioflm inhibition capacity of ebselen was further afrmed through an assay indicating reduction in the hydrophobicity of the bacterial cell surface, a crucial factor in bacterial adhesion and thus inhibiting an initial step in bioflm formation.…”

Section: Ebselen's Efect On Virulence Factors Of Gram-negativementioning

confidence: 99%

Ebselen: A Promising Repurposing Drug to Treat Infections Caused by Multidrug-Resistant Microorganisms

Alves de Lima e Silva,

Rio-Tinto

2024

Interdisciplinary Perspectives on Infectious Diseases

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Bacterial multiresistance to drugs is a rapidly growing global phenomenon. New resistance mechanisms have been described in different bacterial pathogens, threatening the effective treatment of even common infectious diseases. The problem worsens in infections associated with biofilms because, in addition to the pathogen’s multiresistance, the biofilm provides a barrier that prevents antimicrobial access. Several “non-antibiotic” drugs have antimicrobial activity, even though it is not their primary therapeutic purpose. However, due to the urgent need to develop effective antimicrobials to treat diseases caused by multidrug-resistant pathogens, there has been an increase in research into “non-antibiotic” drugs to offer an alternative therapy through the so-called drug repositioning or repurposing. The prospect of new uses for existing drugs has the advantage of reducing the time and effort required to develop new compounds. Moreover, many drugs are already well characterized regarding toxicity and pharmacokinetic/pharmacodynamic properties. Ebselen has shown promise for use as a repurposing drug for antimicrobial purposes. It is a synthetic organoselenium with anti-inflammatory, antioxidant, and cytoprotective activity. A very attractive factor for using ebselen is that, in addition to potent antimicrobial activity, its minimum inhibitory concentration is very low for microbial pathogens.

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“…For this experiment, 10 6 CFU ml −1 overnight culture was treated with different concentrations of Se@ZIF-8NPs (10-1000 µg ml −1 ) and incubated at 28 • C, for 24 h. Post-incubation, spent medium was removed and the cells were treated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), 0.2 mg ml −1 in GM3 medium and incubated for 3 h at 28 • C. After incubation, the spent medium was removed and the crystals were dissolved in 200 µl DMSO. Cell viability was determined by plotting the Se@ZIF-8NPs concentration vs the percentage viability [39,40].…”

Section: Minimum Inhibitory Concentration (Mic) Determinationmentioning

confidence: 99%

Se-functionalized ZIF-8 nanoparticles: synthesis, characterization and disruption of biofilms and quorum sensing in Serratia marcescens

Gokhale,

Patravale,

Pingale

et al. 2024

Biomed. Mater.

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The majority of research on nanomaterials has been concentrated on metal nanoparticles since they are easily made and manipulated. Nanomaterials have shown a wide range of applications in biology. Nevertheless, their bioactivity declines due to their extreme susceptibility to chemical and physical stimuli. The goal of encapsulating these nanomaterials in a matrix is gradually being pursued, which boosts their affordability, stability, and usability. Metal-organic frameworks, often known as MOFs, have the potential to be the best platforms for encapsulating metal nanoparticles due to their well-defined frameworks, persistent porosity, and flexibility in modification. In this investigation, we report the synthesis and optimization of polyvinylpyrrolidone (PVP)-stabilized Se (0) nanoparticles and novel Se@ZIF-8 by chemical method. The sizes and morphologies of Se (0) and Se@ZIF-8 were affected by the ratios of Se/Zn2+ and [hmim]/Zn2+ used. The optimized Se@ZIF-8 nanoparticles exhibited a particle size and zeta potential of 319 nm and -34 mv respectively. Transmission electron microscopy displayed spherical morphology for Se(0) nanoparticles, whereas the surface morphology of novel Se@ZIF-8 nanoparticles was drastically changed to hexagonal shaped structures with smooth surface morphologies in scanning electron microscopy. The DTA, TG/DTG, XRD analysis confirmed the presence of novel Se incorporated ZIF-8 nanoparticulate framework. The synthesized novel Se@ZIF-8 nanoparticles showed efficient antibacterial activity as evidenced by low MIC values. Interestingly, these Se@ZIF-8 NPs not only inhibited biofilm formation in S. marcescens, but also effectively eradicated mature biofilms by degrading the eDNA of the EPS layer. It was observed that Se@ZIF-8 targeted the Quroum Sensing pathway and reduced its associated virulence factors production. This work opens up a different approach of Se@ZIF-8 nanoparticles as novel antibiotics to treat biofilm-associated infections caused by S. marcescens and offer a solution for antimicrobial resistance.

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Combating planktonic and biofilm growth of Serratia marcescens by repurposing ebselen

Cited by 5 publications

References 42 publications

Exploring the efficacy of tryptone-stabilized silver nanoparticles against respiratory tract infection-causing bacteria: a study on planktonic and biofilm forms

Exploring the efficacy of tryptone-stabilized silver nanoparticles against respiratory tract infection-causing bacteria: a study on planktonic and biofilm forms

Ebselen: A Promising Repurposing Drug to Treat Infections Caused by Multidrug-Resistant Microorganisms

Se-functionalized ZIF-8 nanoparticles: synthesis, characterization and disruption of biofilms and quorum sensing in Serratia marcescens

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