Combination assay of lung cancer associated serum markers using surface-enhanced Raman spectroscopy

Song, Chunyuan; Yang, Yanjun; Yang, Boyue; Min, Linghua; Wang, Lianhui

doi:10.1039/c5tb02780b

Cited by 48 publications

(27 citation statements)

References 30 publications

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“…The sensitivity to the environment of the NPs and interparticles coupling enables the uses of LSPRs in biosensing applications [67,68]. Besides, LSPRs are able to greatly amplify the local electromagnetic (EM) fields, making the Au NPs ideal for use in SERS [69,70,71,72,73,74] and MEF [75,76,77]. The small mode volume of LSPRs also increases the photonic local density of states, which can modify the decay rate and quantum efficiency of emitters in the vicinity of Au NPs [78].…”

Section: Optical Diagnostic Based On Au Nanoparticlesmentioning

confidence: 99%

Optical Diagnostic Based on Functionalized Gold Nanoparticles

Zhou

Chen

et al. 2019

IJMS

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Au nanoparticles (NPs) possess unique physicochemical and optical properties, showing great potential in biomedical applications. Diagnostic spectroscopy utilizing varied Au NPs has become a precision tool of in vitro and in vivo diagnostic for cancer and other specific diseases. In this review, we tried to comprehensively introduce the remarkable optical properties of Au NPs, including localized surfaces plasmon resonance (LSPR), surface-enhanced Raman scattering (SERS), and metal-enhanced fluorescence (MEF). Then, we highlighted the excellent works using Au NPs for optical diagnostic applications. Ultimately, the challenges and future perspective of using Au NPs for optical diagnostic were discussed.

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Section: Optical Diagnostic Based On Au Nanoparticlesmentioning

confidence: 99%

Optical Diagnostic Based on Functionalized Gold Nanoparticles

Zhou

Chen

et al. 2019

IJMS

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“…An ultrasensitive SERS substrate was developed for the detection of multiple microRNA (miRNA) biomarkers for lung cancer using molecular beacons attached to the surface of an Ag nanorod array. This sensor was able to detect attomolar concentrations of the miRNA sequences in human serum, creating the potential for use of this substrate as a clinical lung cancer detection mechanism [ 102 , 103 ].…”

Section: Cancermentioning

confidence: 99%

“…This technique was modified further to enable multiplexed detection through the preparation of two different nanotags, containing different Raman reporter molecules and antibodies for either CEA or neuron-specific enolase (NSE). These nanotags were placed in solution with Au-coated magnetic nanoparticles (Fe 3 O 4 , GMNP) and functionalized with antibodies to both biomarkers, creating a sandwich assay with a unique reporter molecule signal for each biomarker [ 102 ]. The LODs were reported as 1.48 and 2.04 pg mL −1 for CEA and NSE, respectively, demonstrating their potential utility as a clinical tool for lung cancer diagnostics.…”

Section: Cancermentioning

confidence: 99%

In Vitro and In Vivo SERS Biosensing for Disease Diagnosis

et al. 2018

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For many disease states, positive outcomes are directly linked to early diagnosis, where therapeutic intervention would be most effective. Recently, trends in disease diagnosis have focused on the development of label-free sensing techniques that are sensitive to low analyte concentrations found in the physiological environment. Surface-enhanced Raman spectroscopy (SERS) is a powerful vibrational spectroscopy that allows for label-free, highly sensitive, and selective detection of analytes through the amplification of localized electric fields on the surface of a plasmonic material when excited with monochromatic light. This results in enhancement of the Raman scattering signal, which allows for the detection of low concentration analytes, giving rise to the use of SERS as a diagnostic tool for disease. Here, we present a review of recent developments in the field of in vivo and in vitro SERS biosensing for a range of disease states including neurological disease, diabetes, cardiovascular disease, cancer, and viral disease.

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“…Recently, surface-enhanced Raman scattering (SERS) based methods for multiple tumor biomarkers detection have got a lot of attentions because of its narrow 'fingerprint' Raman spectrum from analyte molecule, which enables the SERS to be utilized to recognize and quantify various targets from mixture [31]. With the development of various nanomaterials, SERS was utilized for detecting multiple cancer biomarkers successfully [32][33][34][35][36]. For example, in order to detect AFP and Glypican-3, Tang et al has used silver nanoparticle film as SERS substrate to design SERS frequency-shift immunoassay [32].…”

mentioning

confidence: 99%

“…For example, in order to detect AFP and Glypican-3, Tang et al has used silver nanoparticle film as SERS substrate to design SERS frequency-shift immunoassay [32]. By taking advantages of gold nanoflowers and SERS-active magnetic nanoparticles, a sandwich immunoassay was designed by Song et al to detect CEA and NSE, which showed high specificity and sensitivity [33]. Li et al designed an innovative SERS technique using branched DNA-gold nanoaggregates to detect MUC1mucin and nucleolin on breast cancer cell surface [34].…”

mentioning

confidence: 99%

Multiplexing Determination of Cancer-Associated Biomarkers by Surface-Enhanced Raman Scattering Using Ordered Gold Nanohoneycomb Arrays

Liu

Cao

et al. 2017

Bioanalysis

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Aim:Here, a multiplex surface-enhanced Raman scattering (SERS) based assay for simultaneous quantitation of carcinoembryonic antigen (CEA) and α-fetoprotein (AFP) was developed. Methods: SERS tags of nanostars and SERS substrates of nanobowl arrays were functionalized with labeling and capturing antibodies, respectively. In presence of antigens, SERS tags, antigens and SERS substrates formed sandwich structure. Results: The SERS-based technique showed a wide linear range from 0.5 to 100 ng/ml and detection limits were 0.41 and 0.35 ng/ml for CEA and AFP in phosphate-buffered saline buffer, respectively. Analysis results of clinical serum samples using this technique were similar to that shown in phosphatebuffered saline buffer. The LODs were 0.44 and 0.40 ng/ml for CEA and AFP, respectively. Conclusion: The precision and stability of this analysis technique were satisfactory, meanwhile, no obvious cross-reactivity could be found. What's more, it also suggested that this novel multiplex SERS-based technique could be a simple, specific, reliable, sensitive and multiplexed tool for important diagnostic and prognostic applications. Cancer is a latent fatal illness which kills millions of people worldwide, and the number of people dying from cancer continues to rise. If they were detected and cured earlier, many patients who are dying from cancer would have been saved [1,2]. In clinical diagnosis of cancer, detecting tumor biomarkers at the early stage enables early diagnosis of cancer and receives great deal of attentions [3,4]. Among various cancer biomarker detections, quantified detection of serum biomarkers plays a critical role not only in detecting disease at the early stage, but also in tracking disease development after medical treatment [5]. Recently, varieties of immunoassays and immunosensors are devoted to the detection of a single cancer biomarker [6][7][8]. Although they achieved low detection limits, a majority of cancers have more than one biomarker that correlated with their incidence, and some cancer biomarkers are nonspecific to a special cancer, such as α-fetoprotein (AFP) and carcinoembryonic antigen (CEA), CEA and AFP are related to many kinds of cancer, including liver cancer, colorectal cancer, lung cancer, ovarian carcinoma, breast cancer and pancreatic cancer. Moreover, due to biological diversity of man, only one biomarker detection may generate false positive, which is unreliable [9,10]. Therefore, the detection of a single cancer biomarker is inadequate in diagnosing a special cancer. Comparing with the single biomarker test, multiplex immunoassays of cancer biomarkers can promote the screening and diagnosis of some cancer-related illness [11][12][13]. In addition, the simultaneous multiplex assay that detected more than one cancer biomarker in a single experiment possesses remarkable superiorities, such as shorter analytical time, enhanced detecting throughput, higher efficiency, lower detection cost and less sample. Therefore, it would be advantageous to explore an efficient and ultras...

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Combination assay of lung cancer associated serum markers using surface-enhanced Raman spectroscopy

Abstract: A highly sensitive and specific SERS-based combination assay of lung cancer associated serum markers is reported, and the LODs of CEA and NSE in human serum specimens are 1.48 pg mL−1 and 2.04 pg mL−1, respectively.

Cited by 48 publications

References 30 publications

Optical Diagnostic Based on Functionalized Gold Nanoparticles

Optical Diagnostic Based on Functionalized Gold Nanoparticles

In Vitro and In Vivo SERS Biosensing for Disease Diagnosis

Multiplexing Determination of Cancer-Associated Biomarkers by Surface-Enhanced Raman Scattering Using Ordered Gold Nanohoneycomb Arrays

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