Combination effect of lactoalbumin hydrolysate and methyl jasmonate on ginsenoside and polysaccharide production in Panax quinquefolium L. cells cultures

Wang, Juan; Gao, Wenyuan; Zhang, Jian; Huang, Tao; Wen, Tiantian; Huang, Luqi

doi:10.1007/s11738-010-0611-x

Cited by 13 publications

(5 citation statements)

References 16 publications

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“…Thanh et al also found ginsenoside accumulation in ginseng cell cultures was enhanced by elicitation of 200 μM MJ, and the ginsenoside content was two times that of the control [ 25 ]. In the present study, live endophyte not only increased biomass, but also improved ginsenoside accumulation in adventitious root culture, the ginsenoside production was four times that of the control, which was higher than other studies which used MeJA as an elicitor [ 24 , 25 ].…”

Section: Resultscontrasting

confidence: 64%

“…Therefore, the degree of culture biomass inhibition should be considered before choosing an elicitor. Some studies found that the combination of elicitors increased the accumulation of secondary metabolites, such as 100 mg mL −1 lactoalbumin hydrolysate mixed with 2 mg L −1 MeJA, which enhanced ginsenoside accumulation in Panax quinquefolius cells [ 24 ]. To compare elicitation by MeJA and strain LB 5-3 on adventitious root cultures, MeJA, endophytic bacteria and their combination were added during flask culture.…”

Section: Resultsmentioning

confidence: 99%

“…This is possibly because the toxic effect of MeJA inhibited the growth of ginseng adventitious roots and strain LB 5-3 [ 23 ]. Huang et al found that the biomass of Panax quinquefolium L. cells cultures decreased after elicitation with 2 mg L −1 MeJA, but the ginsenoside content improved and was 1.86 times higher than the control group [ 24 ]. Thanh et al also found ginsenoside accumulation in ginseng cell cultures was enhanced by elicitation of 200 μM MJ, and the ginsenoside content was two times that of the control [ 25 ].…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Endophytic Bacteria Isolated from Panax ginseng Improves Ginsenoside Accumulation in Adventitious Ginseng Root Culture

Song

Yin

et al. 2017

Molecules

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Ginsenoside is the most important secondary metabolite of ginseng. Natural sources of wild ginseng have been overexploited. Although root culture could reduce the length of the growth cycle of ginseng, the number of ginsenosides is fewer and their contents are lower in adventitious roots of ginseng than that in ginseng cultivated in the field. In this study, we investigated the effects of endophytic bacterial elicitors on biomass and ginsenoside production in adventitious roots cultures of Panax ginseng. Endophyte LB 5-3 as an elicitor could increase biomass and ginsenoside accumulation in ginseng adventitious root culture. After 6 days elicitation with a 10.0 mL of strain LB 5-3, the content of total ginsenoside was 2.026 mg g−1 which was four times more than that in unchallenged roots. The combination of methyl jasmonate and strain LB 5-3 had a negative effect on ginseng adventitious root growth and ginsenoside production. The genomic DNA of strain LB 5-3 was sequenced, and was found to be most closely related to Bacillus altitudinis (KX230132.1). The challenged ginseng adventitious root extracts exerted inhibitory effect against the HepG2 cells, which IC50 value was 0.94 mg mL−1.

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Section: Resultscontrasting

confidence: 64%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Endophytic Bacteria Isolated from Panax ginseng Improves Ginsenoside Accumulation in Adventitious Ginseng Root Culture

Song

Yin

et al. 2017

Molecules

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“…However, efforts to increase bioactive compounds need to be continued during the culture. MeJA as an elicitor has a good elicitation role in many culture systems [4,[23][24][25][26], including O. elatus AR culture. Jiang et al [4] indicated that MeJA (200 µM) increases bioactive compound production in the batch culture of O. elatus ARs.…”

Section: Discussionmentioning

confidence: 99%

Improving Flavonoid Accumulation of Bioreactor-Cultured Adventitious Roots in Oplopanax elatus Using Yeast Extract

Jin

Wang

et al. 2023

Plants

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Oplopanax elatus is an endangered medicinal plant, and adventitious root (AR) culture is an effective way to obtain its raw materials. Yeast extract (YE) is a lower-price elicitor and can efficiently promote metabolite synthesis. In this study, the bioreactor-cultured O. elatus ARs were treated with YE in a suspension culture system to investigate the elicitation effect of YE on flavonoid accumulation, serving for further industrial production. Among YE concentrations (25-250 mg/L), 100 mg/L YE was the most suitable for increasing the flavonoid accumulation. The ARs with various ages (35-, 40-, and 45-day-old) responded differently to YE stimulation, where the highest flavonoid accumulation was found when 35-day-old ARs were treated with 100 mg/L YE. After YE treatment, the flavonoid content increased, peaked at 4 days, and then decreased. By comparison, the flavonoid content and antioxidant activities in the YE group were obviously higher than those in the control. Subsequently, the flavonoids of ARs were extracted by flash extraction, where the optimized extraction process was: 63% ethanol, 69 s of extraction time, and a 57 mL/g liquid–material ratio. The findings provide a reference for the further industrial production of flavonoid-enriched O. elatus ARs, and the cultured ARs have potential application for the future production of products.

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“…Transgenic ginseng plants with stable heredity and high saponin content productivity need to be further investigated. Main achievements of ginseng tissue and cell cultures in recent years are summarized in Table 2 92 , 94 , 169 , 173 , 178 , 184 , 186 , 187 , 188 , 191 , 192 , 193 , 194 , 195 , 196 , 197 , 198 , 199 , 200 , 201 , 202 , 203 , 204 , 205 , 206 , 207 , 208 , 209 , 210 , 211 , 212 , 213 , 214 , 215 .…”

Section: Biotechnological Approaches For Ginsenoside Productionmentioning

confidence: 99%

Ginsenosides in Panax genus and their biosynthesis

Hou

Wang

Zhao

et al. 2021

Acta Pharmaceutica Sinica B

174

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Ginsenosides are a series of glycosylated triterpenoids which belong to protopanaxadiol (PPD)-, protopanaxatriol (PPT)-, ocotillol (OCT)- and oleanane (OA)-type saponins known as active compounds of Panax genus. They are accumulated in plant roots, stems, leaves, and flowers. The content and composition of ginsenosides are varied in different ginseng species, and in different parts of a certain plant. In this review, we summarized the representative saponins structures, their distributions and the contents in nearly 20 Panax species, and updated the biosynthetic pathways of ginsenosides focusing on enzymes responsible for structural diversified ginsenoside biosynthesis. We also emphasized the transcription factors in ginsenoside biosynthesis and non-coding RNAs in the growth of Panax genus plants, and highlighted the current three major biotechnological applications for ginsenosides production. This review covered advances in the past four decades, providing more clues for chemical discrimination and assessment on certain ginseng plants, new perspectives for rational evaluation and utilization of ginseng resource, and potential strategies for production of specific ginsenosides.

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Combination effect of lactoalbumin hydrolysate and methyl jasmonate on ginsenoside and polysaccharide production in Panax quinquefolium L. cells cultures

Cited by 13 publications

References 16 publications

Endophytic Bacteria Isolated from Panax ginseng Improves Ginsenoside Accumulation in Adventitious Ginseng Root Culture

Endophytic Bacteria Isolated from Panax ginseng Improves Ginsenoside Accumulation in Adventitious Ginseng Root Culture

Improving Flavonoid Accumulation of Bioreactor-Cultured Adventitious Roots in Oplopanax elatus Using Yeast Extract

Ginsenosides in Panax genus and their biosynthesis

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