Combination effects of mesenchymal stem cells transplantation and anodal transcranial direct current stimulation on a cuprizone-induced mouse model of multiple sclerosis

Mojaverrostami, Sina; Khadivi, Farnaz; Zarini, Davood; Mohammadi, Alireza

doi:10.1007/s10735-022-10092-8

Cited by 8 publications

(3 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently there has been a burgeoning interest in tDCS applications in several neurological and psychological diseases such as depression ( Zhang et al, 2021 ), attention deficit hyperactivity disorder (ADHD) ( Breitling et al, 2016 ), stroke ( O'Shea et al, 2014 ), Parkinson’s Disease (PD) ( Ishikuro et al, 2018 ) and MS. In the latter, preclinical studies have even reported instances of tDCS-assisted neuronal remyelination ( Mojaverrostami et al, 2022 ). In this specific study, we used advanced imaging techniques to quantify the neuronal influences of left anodal DLPFC tDCS as result of the external stimulation itself (simultaneous effects) and of aCT paired treatment via repeated sessions (cumulative effects), in MS patients.…”

Section: Discussionmentioning

confidence: 99%

Simultaneous and cumulative effects of tDCS on cerebral metabolic rate of oxygen in multiple sclerosis

Muccio,

Pilloni,

Walton Masters

et al. 2024

Front. Hum. Neurosci.

View full text Add to dashboard Cite

IntroductionTranscranial direct current stimulation (tDCS) is a non-invasive neuromodulation technique with simultaneous (during stimulation) and cumulative effects (after repeated sessions) on blood flow and neuronal metabolism. These effects remain mostly unclear especially in multiple sclerosis (MS). This work aims to elucidate brain metabolic and hemodynamic underpinnings of tDCS and its potential therapeutic impact in MS patients using quantitative tDCS-MRI.MethodsMS participants (n = 20; age = 45.4 ± 12.3 years, 7 males) underwent 3 T MRI scans before and after 20 daily sessions of dorsolateral prefrontal cortex (DLFPC) tDCS (2.0 mA, left anodal) paired with adaptive cognitive training (aCT). During both visits, imaging measurements of cerebral blood flow (CBF), cerebral venous blood oxygenation (Yv) and calculated cerebral metabolic rate of oxygen (CMRO2) were obtained at pre-tDCS, during-tDCS and post-tDCS.ResultsAt baseline, significant increase from pre- to during-tDCS was observed in CMRO2 (7.6%; p = 0.002), CBF (11.0%; p < 0.0001) and Yv (1.9%; p = 0.006). At follow up, we observed an increase in pre-tDCS CMRO2 (140.59 ± 13.83 μmol/100 g/min) compared to baseline pre-tDCS levels (128.30 ± 14.00 μmol/100 g/min; p = 0.006). Sustained elevations in CMRO2 and CBF into post-tDCS were also observed (tDCS lingering effects). Cumulative tDCS effects were observed in the form of sustained elevations in CMRO2 and CBF in pre-tDCS follow up, reaching the magnitudes measured at baseline during-tDCS.DiscussionTDCS induces an acute surge in metabolic activity persisting immediately after the stimulation is removed. Moreover, treatment composed of repeated tDCS-aCT paired sessions contributes to establishing long-lasting increases in neuronal activity.

show abstract

Section: Discussionmentioning

confidence: 99%

Simultaneous and cumulative effects of tDCS on cerebral metabolic rate of oxygen in multiple sclerosis

Muccio,

Pilloni,

Walton Masters

et al. 2024

Front. Hum. Neurosci.

View full text Add to dashboard Cite

show abstract

“…Presently, there is no effective treatment available for MS. Current pharmacological therapies only offer symptom management and slow down the disease progression by modulating the immune response or inflammatory cascades [26].…”

Section: Discussionmentioning

confidence: 99%

“…MSCs have also been reported to exhibit remarkable regeneration potential, self-renewal capabilities, and immunomodulatory features [2,12]. MSCs are also effective to treat neurological disorders and exhibit neuroprotective features which make them an attractive candidate for cell-based therapies [20,26].…”

Section: Discussionmentioning

confidence: 99%

Overexpression of OLIG2 and MYT1L Transcription Factors Enhance the Differentiation Potential of Human Mesenchymal Stem Cells into Oligodendrocytes

Fahim,

Ishaque,

Ramzan

et al. 2023

CIMB

View full text Add to dashboard Cite

Background: Demyelinating diseases represent a broad spectrum of disorders and are characterized by the loss of specialized glial cells (oligodendrocytes), which eventually leads to neuronal degeneration. Stem cell-based regenerative approaches provide therapeutic options to regenerate demyelination-induced neurodegeneration. Objectives: The current study aims to explore the role of oligodendrocyte-specific transcription factors (OLIG2 and MYT1L) under suitable media composition to facilitate human umbilical-cord-derived mesenchymal stem cells (hUC-MSCs) differentiation toward oligodendrocyte for their potential use to treat demyelinating disorders. Methodology: hUC-MSCs were isolated, cultured, and characterized based on their morphological and phenotypic characteristics. hUC-MSCs were transfected with OLIG2 and MYT1L transcription factors individually and in synergistic (OLIG2 + MYT1L) groups using a lipofectamine-based transfection method and incubated under two different media compositions (normal and oligo induction media). Transfected hUC-MSCs were assessed for lineage specification and differentiation using qPCR. Differentiation was also analyzed via immunocytochemistry by determining the expression of oligodendrocyte-specific proteins. Results: All the transfected groups showed significant upregulation of GFAP and OLIG2 with downregulation of NES, demonstrating the MSC commitment toward the glial lineage. Transfected groups also presented significant overexpression of oligodendrocyte-specific markers (SOX10, NKX2.2, GALC, CNP, CSPG4, MBP, and PLP1). Immunocytochemical analysis showed intense expression of OLIG2, MYT1L, and NG2 proteins in both normal and oligo induction media after 3 and 7 days. Conclusions: The study concludes that OLIG2 and MYT1L have the potential to differentiate hUC-MSCs into oligodendrocyte-like cells, which is greatly facilitated by the oligo induction medium. The study may serve as a promising cell-based therapeutic strategy against demyelination-induced neuronal degeneration.

show abstract

Advances in Material‐Assisted Electromagnetic Neural Stimulation

Sun,

Xiao,

Chen

et al. 2024

Advanced Materials

View full text Add to dashboard Cite

Bioelectricity plays a crucial role in organisms, being closely connected to neural activity and physiological processes. Disruptions in the nervous system can lead to chaotic ionic currents at the injured site, causing disturbances in the local cellular microenvironment, impairing biological pathways, and resulting in a loss of neural functions. Electromagnetic stimulation has the ability to generate internal currents, which can be utilized to counter tissue damage and aid in the restoration of movement in paralyzed limbs. By incorporating implanted materials, electromagnetic stimulation can be targeted more accurately, thereby significantly improving the effectiveness and safety of such interventions. Currently, there have been significant advancements in the development of numerous promising electromagnetic stimulation strategies with diverse materials. This review provides a comprehensive summary of the fundamental theories, neural stimulation modulating materials, material application strategies, and pre‐clinical therapeutic effects associated with electromagnetic stimulation for neural repair. It offers a thorough analysis of current techniques that employ materials to enhance electromagnetic stimulation, as well as potential therapeutic strategies for future applications.This article is protected by copyright. All rights reserved

show abstract

Combination effects of mesenchymal stem cells transplantation and anodal transcranial direct current stimulation on a cuprizone-induced mouse model of multiple sclerosis

Cited by 8 publications

References 55 publications

Simultaneous and cumulative effects of tDCS on cerebral metabolic rate of oxygen in multiple sclerosis

Simultaneous and cumulative effects of tDCS on cerebral metabolic rate of oxygen in multiple sclerosis

Overexpression of OLIG2 and MYT1L Transcription Factors Enhance the Differentiation Potential of Human Mesenchymal Stem Cells into Oligodendrocytes

Advances in Material‐Assisted Electromagnetic Neural Stimulation

Contact Info

Product

Resources

About