Background: There is a considerable number of homosexual and bisexual people in the Chinese population, while their health conditions have not received sufficient attention compared with general Chinese. Referring to World Health Organization (WHO)’s definition, holistic health is a comprehensive concept incorporating physical, mental, and social well-being. However, existing research seems inadequate and unbalanced on the holistic health of this population, especially many studies only focus on sexually transmitted infections (STI) and relating preventions. Apart from these, the health status of these sexual minorities from a holistic perspective remains unclear. Therefore, this scoping review aims to comprehensively examine all available literature and will be the first to map the existing evidence on the holistic health of homosexual and bisexual Chinese.Methods: This proposed scoping review has been structured according to the Joanna Briggs Institute (JBI) recommended framework, which was proposed by Arksey and O’Malley and further enhanced by Levac, et al. Pilot searches of PubMed in English and SinoMed in Chinese were completed to identify all relevant search terms or keywords, then a comprehensive search strategy has been carried out across all relevant databases centering on health care, psychology, nursing and social science, including both English and traditional as well as simplified Chinese electronic databases. Screening and study selection will be independently conducted by two reviewers, and consulting a third senior reviewer based on consensus needs. Data will be extracted using a structured data form referring to the Cochrane template, and a narrative synthesis of the findings will be used.Discussion: There is no systematically reviewed evidence available or underway review on the holistic health of either homosexual or bisexual Chinese, so this scoping review will serve as a most comprehensive way to map the existing evidence and draw a big picture of their health conditions. Results of this review can be used to inform scholars of existing health issues and other major needs, then to provide scientific references for the development of person-centered culturally sensitive programs with Chinese characteristics.Systematic review registration: This protocol has been registered within Open Science Framework (https://osf.io/82r7z) on April 27, 2020.