Combination of an Immunomagnetic Separation Method and a Chromogenic Oligonucleotide Array for the Detection of Beer-Spoilage Lactic Acid Bacteria

Chiang, Yu-Cheng; Liao, Wanwen; Lin, Ching-Yi; Lin, Chi-Ying; Tsen, Hau‐Yang; Yeh, Chao-Chun; Lee, Shih‐Chieh; Wang, Hsien-Huang

doi:10.1094/asbcj-2013-0126-01

Journal of the American Society of Brewing Chemists

2013

DOI: 10.1094/asbcj-2013-0126-01

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Combination of an Immunomagnetic Separation Method and a Chromogenic Oligonucleotide Array for the Detection of Beer-Spoilage Lactic Acid Bacteria

Yu-Cheng Chiang

Wanwen Liao

Ching-Yi Lin

et al.

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2020

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“…In this study, we found that Enterococcus was abundant in CON silage which showed a low level of ensiling quality. Butyric acid-producing bacteria (BAB), such as Clostridia and Bacillus, are considered undesirable in silage (Muck, 2013). Both genera of bacteria can convert lactic acid into butyric acid, and some species of these genera can lead to the presence of pathogenic toxins in silage (Driehuis and Elferink, 2000).…”

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The Microbiota Dynamics of Alfalfa Silage During Ensiling and After Air Exposure, and the Metabolomics After Air Exposure Are Affected by Lactobacillus casei and Cellulase Addition

Niu

Tong

et al. 2020

Front. Microbiol.

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Both inoculants treatment and enzyme treatment promote the reproduction of lactic acid bacteria (LAB) to produce enough lactic acid to lower pH in silage. The present study investigated the microbial community and metabolome in cellulase, Lactobacillus casei, and air treated alfalfa silage. Chopped and wilted alfalfa (first cutting, 29% dry matter) was ensiled without (CON) or with L. casei (1 × 106 cfu g–1 fresh matter) (LC) or cellulase (20,000IU, 0.5% of fresh matter) (CE) for 56 days, then exposed to air for 3 days (PO). Greater ensiling quality was observed in LC and CE, which had lower pH and higher lactic acid content than CON at 56 days of ensiling and 3 days post-oxygen exposure. Air exposure was associated with decreased lactic acid concentrations and increased yeast and mold counts in all silages. SEM showed that the structure of leaf epicuticular wax crystals were intact in fresh alfalfa, totally decomposed in CON silage, and partly preserved in CE and LC silage. Gas chromatography mass spectrometry revealed that 196 metabolites and 95 differential concentration were present in the 3 days air exposure samples. Most of these metabolites, mainly organic acids, polyols, ketones, aldehydes, are capable of antimicrobial activity. The bacterial communities were obviously different among groups and Lactobacillus developed to a dominant status in all silages. Lactobacillus became dominant in bacterial communities of LC and CE silages from days 7 to 56, and their relative abundances reached 94.17–83.93% at day 56, respectively. For CON silage, until day 56, Lactobacillus dominated the bacterial community with abundance of 75.10%. After 3 days of oxygen exposure, Lactobacillus and Enterococcus were predominant in CON, and Lactobacillus remained dominant in LC and CE silages. The results indicated that, compared to untreated silages, L. casei could be a priority inoculant for alfalfa silage to boost Lactobacillus abundance and improve fermentation quality. Our high-throughput sequencing and gas chromatography mass spectrometry results provide a deep insight into the bacterial community and metabolites in alfalfa silage.

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mentioning

confidence: 99%

The Microbiota Dynamics of Alfalfa Silage During Ensiling and After Air Exposure, and the Metabolomics After Air Exposure Are Affected by Lactobacillus casei and Cellulase Addition

Niu

Tong

et al. 2020

Front. Microbiol.

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Combination of an Immunomagnetic Separation Method and a Chromogenic Oligonucleotide Array for the Detection of Beer-Spoilage Lactic Acid Bacteria

Cited by 1 publication

References 22 publications

The Microbiota Dynamics of Alfalfa Silage During Ensiling and After Air Exposure, and the Metabolomics After Air Exposure Are Affected by Lactobacillus casei and Cellulase Addition

The Microbiota Dynamics of Alfalfa Silage During Ensiling and After Air Exposure, and the Metabolomics After Air Exposure Are Affected by Lactobacillus casei and Cellulase Addition

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