Combination of aptamer with gold nanoparticles for electrochemical signal amplification: Application to sensitive detection of platelet-derived growth factor

Wang, Jing; Meng, Wenying; Zheng, Xiaofeng; Liu, Shanli; Li, Genxi

doi:10.1016/j.bios.2008.08.030

Cited by 123 publications

(24 citation statements)

References 37 publications

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“…Sandwich schemes for PDGF and IgE, based on aptamertethered AuNPs, were proposed in Refs. [77] and [78], respectively. In both cases AuNps were used for loading the redox label, ruthenium hexamine and methylene blue, respectively.…”

Section: Metal Nanoparticlesmentioning

confidence: 98%

Electrochemical nanomaterial-based nucleic acid aptasensors

Palchetti

Mascini

2012

Anal Bioanal Chem

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Recent progress in the development of electrochemical nanomaterial-aptamer-based biosensors is summarized. Aptamers are nucleic acid ligands that can be generated against amino acids, drugs, proteins, and other molecules. They are isolated from a large random library of synthetic nucleic acids by an iterative process of binding, separation, and amplification, called systematic evolution of ligands by exponential enrichment (SELEX). In this review, different methods of integrating aptamers with different nanomaterials and nanoparticles for electrochemical biosensing application are described.

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Section: Metal Nanoparticlesmentioning

confidence: 98%

Electrochemical nanomaterial-based nucleic acid aptasensors

Palchetti

Mascini

2012

Anal Bioanal Chem

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“…Wang and co-workers [27] reported an aptamer-based sensor for the detection of the platelet-derived growth factor (PDGF) also using gold nanoparticles (Au NPs). [Ru (NH 3 ) 5 Cl] 2+ complex, electrostatically immobilised onto the strand of each aptamer, allowed the detection by CV, enabling a detection limit of 10 fM for purified samples and 1 pM for contaminated-ridden samples or undiluted blood serum.…”

Section: Labelled Aptamer-based Sensorsmentioning

confidence: 99%

Electrochemical aptamer-based sensors

2010

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The valuable properties of aptamers, such as specificity, sensitivity, stability, cost-effectiveness and design flexibility, have favoured their use as biorecognition elements in biosensor development. These synthetic affinity probes can be developed for almost any target molecule, covering a wide range of applications in fields such as clinical diagnosis and therapy, environmental monitoring and food control. The combination of aptamers with highperformance electrochemical transducers, with their inherent high sensitivities, fast response times and simple equipment, has already provided several electrochemical aptamer-based sensors. Moreover, the small size and versatility of aptamers allow efficient immobilisations in high-density monolayers, an important feature towards miniaturisation and integration of compact electrochemical devices. This review describes the state-of-the-art of electrochemical aptamer-based sensors, entering into the details of the different strategies and types of electrochemical transduction and also considering their advantages when applied to the analysis of complex matrices.

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“…Different methods for signal enhancement have been investigated, such as enzyme labeling [4], rolling circle amplification [5] and nanomaterial labeling [6]. Among these methods, nanomaterial labeling has gaining growing interest due to the intrinsic advantages of nanomaterials, such as low cost, good thermal stability and large surface area [7].…”

Section: Introductionmentioning

confidence: 99%