Combination of atmospheric and room temperature plasma (ARTP) mutagenesis, genome shuffling and dimethyl sulfoxide (DMSO) feeding to improve FK506 production in Streptomyces tsukubaensis

Ye, Liting; Ye, Ruifang; Hu, Fengxian; Wang, Guozhu

doi:10.1007/s10529-021-03154-6

Cited by 15 publications

(14 citation statements)

References 41 publications

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“…Gao et al, 22 As mentioned above, the mutation and improvement of the strain by ARTP is essentially to significantly increase the activity of related enzymes in the strain, that is, to affect the biological effects of the internal factors of the strain. 21,22, 92,94,125,126 Han et al, 120 used Corynebacterium glutamicum as the starting strain and ARTP as the mutagenesis strategy, and structural variations at the genomic level were found within each mutant screened. Among them, the number of SNP and In Del in the mutant is small, indicating that it reduced feedback inhibition regulation and significantly increased production potency.…”

Section: Artp Mutagenic Biosynthesis Of Enzymesmentioning

confidence: 99%

“…Ye et al, 92 performed genetic rearrangement of Streptomyces tsukubaensis by ARTP mutagenesis, which was repeated three times to obtain mutant strains with increased production of the macrocyclic polyketide compound FK506. The expression levels of fkbO, fkbP, dahp, pccB, and prpE genes were upregulated in the mutants compared with the starting strain.…”

Section: Mutagenesis Of Medicine-producing Strains By Artp Technologymentioning

confidence: 99%

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Recent progress of atmospheric and room‐temperature plasma as a new and promising mutagenesis technology

Li,

Shen,

Ding

et al. 2024

Cell Biochemistry & Function

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At present, atmospheric and room‐temperature plasma (ARTP) is regarded as a new and powerful mutagenesis technology with the advantages of environment‐friendliness, operation under mild conditions, and fast mutagenesis speed. Compared with traditional mutagenesis strategies, ARTP is used mainly to change the structure of microbial DNA, enzymes, and proteins through a series of physical, chemical, and electromagnetic effects with the organisms, leading to nucleotide breakage, conversion or inversion, causing various DNA damages, so as to screen out the microbial mutants with better biological characteristics. As a result, in recent years, ARTP mutagenesis and the combination of ARTP with traditional mutagenesis have been widely used in microbiology, showing great potential for application. In this review, the recent progress of ARTP mutagenesis in different application fields and bottlenecks of this technology are systematically summarized, with a view to providing a theoretical basis and technical support for better application. Finally, the outlook of ARTP mutagenesis is presented, and we identify the challenges in the field of microbial mutagenesis by ARTP.

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Section: Artp Mutagenic Biosynthesis Of Enzymesmentioning

confidence: 99%

Section: Mutagenesis Of Medicine-producing Strains By Artp Technologymentioning

confidence: 99%

Recent progress of atmospheric and room‐temperature plasma as a new and promising mutagenesis technology

Li,

Shen,

Ding

et al. 2024

Cell Biochemistry & Function

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“…spinosa that can produce a high spinosad yield (373 mg/L) . In another study, atmospheric and room temperature plasma and genome shuffling mutagenesis tools were combined to produce a Streptomyces tsukubaensis R3-C4 mutant that can produce 335 mg/L FK506, which is 2.6 times greater than the FK506 yield of the wild-type strain . However, screening for useful mutants is a labor-intensive process.…”

Section: Introductionmentioning

confidence: 99%

“…17 In another study, atmospheric and room temperature plasma and genome shuffling mutagenesis tools were combined to produce a Streptomyces tsukubaensis R3-C4 mutant that can produce 335 mg/L FK506, which is 2.6 times greater than the FK506 yield of the wild-type strain. 18 However, screening for useful mutants is a labor-intensive process. Omics technologies enable the monitoring of biomolecule abundance, including variations between biological states or highly related species.…”

Section: Introductionmentioning

confidence: 99%

Exploring a High-Efficiency Genetic Transformation System for Engineering Saccharopolyspora pogona ASAGF58 To Improve Butenyl-Spinosyn Production

Pang

Guo

et al. 2023

ACS Agric. Sci. Technol.

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Butenyl-spinosyn is a potent insecticide potentially useful as a broad-spectrum pesticide. Because it is relatively nontoxic to mammals and does not damage the environment, there has been considerable interest in the use of butenyl-spinosyn for increasing agricultural production. However, genetically engineering Saccharopolyspora pogona ASAGF58 to increase its relatively low butenyl-spinosyn content remains challenging because it cannot be transformed efficiently. In this study, genes encoding novel methyltransferases (04455 and 28970) were identified in the Sa. pogona ASAGF58 genome through a bioinformatic-based analysis. Additionally, the transformation efficiency increased by 5.8-and 16.4-fold when foreign DNA was pre-methylated in ET-28970 and ET-04455, respectively, through bypassing of the restriction−modification system. A comparative proteomic analysis of Sa. pogona and Saccharopolyspora spinosa revealed that acetyl-CoA synthetase may be useful for improving butenyl-spinosyn production. The fermentation results indicated that compared with the wild-type butenyl-spinosyn content, overexpressing the acetyl-CoA synthetase gene increased butenyl-spinosyn production by 2-fold. The findings presented herein suggest that the strategy employed in this study may be applicable for the genetic engineering of other nonmodel Saccharopolyspora strains and increase target product yields.

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“…Instead of using ask fermentation, a high-throughput screening technique using 96-well plates was developed, which eliminates a signi cant proportion of shaking and increases the screening scale but is still time-consuming (Gao et al 2010a). In this paper, Atmospheric Room Temperature Plasma (ARTP) technology was employed to construct a mutation library, which is a novel and rapid mutation tool for microbial evolution engineering (Li et al 2021;Liu et al 2020;Qiu et al 2021;Ye et al 2021). In combination with the rapid colony analyses, the culture was continuously bred one generation after another in the hope of obtaining a superior production strain.…”

Section: Introductionmentioning

confidence: 99%

Screening of a high-yield strain of avermectin B1a by colony analysis in situ

Gou

Li²,

et al. 2022

Int Microbiol

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Avermectin, an agricultural antibiotic, is widely used as an agricultural insecticide and an important lead compound of antibiotics. It is manufactured by Streptomyces avermitilis through fermentation. Manufacturers pay special attention to screening for strains with high fermentation capacity based on morphological properties of the colony and by the result of shake ask fermentation. These traditional screening methods are time-consuming, labor-intensive and require specialized equipment. Moreover, evaluation of colony appearance is highly subjective. To improve and accelerate the screening process, we developed a rapid in situ screening method. Forty-four strains isolated naturally from the spores of industrial high-yielding strains were studied. The data show that the colony fermentation titer is highly correlated with the yield from the shake ask fermentation of avermectin, and the Pearson R is 0.990. The total titer of avermectins by shake ask fermentation is also highly correlated with the B 1a titer (Pearson R is 0.994). This result also shows that strains can be quickly screened by analyzing the colony titer.Pigment rings of the colonies that appeared after growing and maturing on the new medium plate were analyzed. The chosen colonies were directly marked and punched and then extracted with methanol. The fermentation ability can be evaluated by measuring the absorbance at 245 nm. This methodology can be applied in both natural breeding and mutation breeding conditions. By continuously breeding from 2008 to 2020, the ask titer of avermectin B 1a increased from 4582±483 µg/mL to 9197 ± 1134 µg/mL.

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Combination of atmospheric and room temperature plasma (ARTP) mutagenesis, genome shuffling and dimethyl sulfoxide (DMSO) feeding to improve FK506 production in Streptomyces tsukubaensis

Abstract: Combination of Atmospheric and Room Temperature Plasma (ARTP) mutagenesis, Genome shuffling and Dimethyl sulfoxide (DMSO) feeding to improve FK506 production in Streptomyces tsukubaensis

Cited by 15 publications

References 41 publications

Recent progress of atmospheric and room‐temperature plasma as a new and promising mutagenesis technology

Recent progress of atmospheric and room‐temperature plasma as a new and promising mutagenesis technology

Exploring a High-Efficiency Genetic Transformation System for Engineering Saccharopolyspora pogona ASAGF58 To Improve Butenyl-Spinosyn Production

Screening of a high-yield strain of avermectin B1a by colony analysis in situ

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