Combination of Bifidobacterium breve and antibiotics against Clostridioides difficile: effect of the time interval of combination on antagonistic activity

Yang, Jingpeng; Li, Yanan; Meng, Lingtong

doi:10.1007/s10123-023-00340-6

Cited by 3 publications

(2 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Meanwhile, we tested the effect of probiotics and antibiotics on C. difficile cells at different intervals of combination and found that the intervals had different effects on growth, biofilm production, toxin production, and virulence gene expression levels of C. difficile cells. 134 The issue of the interval between co-administrations is a current concern for many clinicians and nutritionists that needs to be solved, and a clear and reasonable interval can help improve the accuracy of CDI prevention and treatment.…”

Section: An Important Basis For the Selection Of “Bacteria–drug Combi...mentioning

confidence: 99%

Strategies for applying probiotics in the antibiotic management ofClostridioides difficileinfection

Yang,

Meng,

et al. 2023

Food Funct.

Self Cite

View full text Add to dashboard Cite

show abstract

Section: An Important Basis For the Selection Of “Bacteria–drug Combi...mentioning

confidence: 99%

Strategies for applying probiotics in the antibiotic management ofClostridioides difficileinfection

Yang,

Meng,

et al. 2023

Food Funct.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Combinational therapies [ 1,2 ] are increasingly becoming a trend in clinic due to the improvement of the therapeutic effect and reduction of patient medication costs. Salt formation between two ionizable drugs, as one of the common strategies for combination medication, could enhance the therapeutic effect of the drugs, allow for combination of two drugs in a single preparation with lowering the frequency of drug administration, [ 3,4 ] and modulate their physicochemical properties such as stability, solubility and bioavailability etc.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Improvements of Physicochemical Properties and Anti‐Microbial Activity of Berberine via Forming Salt with Gallic Acid

Chen,

Yang,

Ren

et al. 2024

Cryst. Res. Technol.

View full text Add to dashboard Cite

Implementing combination medication through crystal engineering technology receives increasing attention from researchers due to improvements in the clinical treatment effects as well as the physicochemical properties of the drug. Berberine (BER) is commonly used to treat gastroenteritis and bacterial diarrhea, with the problems of poor solubility and low oral bioavailability. Recently, it is found that gallic acid (GA) also has anti‐inflammatory and anti‐bacterial activities. Therefore, if the above‐mentioned two ingredients can be combined, it is possible to enhance the therapeutic effects and physicochemical properties of BER. Inspired by this, 8‐hydroxy‐7,8‐dihydroberberine (8H‐HBER) in this study is employed to react with gallic acid to yield BER gallate dihydrate (2BER‐2GA‐2 W). Furthermore, dissolution experiments demonstrate that the maximum apparent solubility (MAS) of 2BER‐2GA‐2 W in dilute hydrochloric acid solution medium (pH 1.2) has increased by 7 times compared to the commercial form of BER, because of avoiding the common‐ion effect. Moreover, 2BER‐2GA‐2 W has also obviously enhanced stability relative to the commercial form of BER. In addition, 2BER‐2GA‐2 W has a better inhibitory effect on Staphylococcus aureus (S. aureus) relative to the commercial form of BER. Hence, 2BER‐2GA‐2 W will be a promising solid‐state form of BER for its further development.

show abstract

Symbiotic biofilms formed by Clostridioides difficile and bacteroides thetaiotaomicron in the presence of vancomycin

Yang,

Rui,

Zhong

et al. 2024

Gut Microbes

View full text Add to dashboard Cite

Vancomycin (VAN) treatment in Clostridioides difficile infection (CDI) suffers from a relatively high rate of recurrence, with a variety of reasons behind this, including biofilm-induced recurrent infections. C. difficile can form monophyletic or symbiotic biofilms with other microbes in the gut, and these biofilms protect C. difficile from being killed by antibiotics. In this study, we analyzed the ecological relationship between Bacteroides thetaiotaomicron and C. difficile and their formation of symbiotic biofilm in the VAN environment. The production of symbiotic biofilm formed by C. difficile and B. thetaiotaomicron was higher than that of C. difficile and B. thetaiotaomicron alone in the VAN environment. In symbiotic biofilms, C. difficile was characterized by increased production of the toxin protein TcdA and TcdB, up-regulation of the expression levels of the virulence genes tcdA and tcdB , enhanced bacterial cell swimming motility and c-di-GMP content, and increased adhesion to Caco-2 cells. The scanning electron microscope (SEM) combined with confocal laser scanning microscopy (CLSM) results indicated that the symbiotic biofilm was elevated in thickness, dense, and had an increased amount of mixed bacteria, while the fluorescence in situ hybridization (FISH) probe and plate colony counting results further indicated that the symbiotic biofilm had a significant increase in the amount of C. difficile cells, and was able to better tolerate the killing of the simulated intestinal fluid. Taken together, C. difficile and B. thetaiotaomicron become collaborative in the VAN environment, and targeted deletion or attenuation of host gut B. thetaiotaomicron content may improve the actual efficacy of VAN in CDI treatment.

show abstract

Combination of Bifidobacterium breve and antibiotics against Clostridioides difficile: effect of the time interval of combination on antagonistic activity

Cited by 3 publications

References 22 publications

Strategies for applying probiotics in the antibiotic management ofClostridioides difficileinfection

Strategies for applying probiotics in the antibiotic management ofClostridioides difficileinfection

Simultaneous Improvements of Physicochemical Properties and Anti‐Microbial Activity of Berberine via Forming Salt with Gallic Acid

Symbiotic biofilms formed by Clostridioides difficile and bacteroides thetaiotaomicron in the presence of vancomycin

Contact Info

Product

Resources

About