Combination of bioanalytical approaches and quantitative proteomics for the elucidation of the toxicity mechanisms associated to TiO2 nanoparticles exposure in human keratinocytes

Montalvo-Quirós, Sandra; Luque-García, José L.

doi:10.1016/j.fct.2019.03.036

Cited by 20 publications

(15 citation statements)

References 45 publications

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“…Stata 15.1 (available in www.stata.com ) was used to conduct meta-analysis, and the random-effect model [19] was chosen. Unstandardized mean difference between the experimental and control group was used to calculate the effect size as follows: Var d s n s n n n n n n n (2) where D is the effect size, Var(d) is the variance of D, 1 x and 2…”

Section: Meta-analysismentioning

confidence: 99%

“…Titanium dioxide nanoparticles (nano-TiO 2 , < 100 nm) are widely used, in industry, technology, and consumer products [1,2] products, and indirectly through environmental exposure to unintentionally released nano-TiO 2 . [1,10] Nano-TiO 2 can also have impact on aquatic organisms exposed to increasing levels of nanoparticles in water and sediments.…”

Section: Introductionmentioning

confidence: 99%

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Rethinking Nano‐TiO₂ Safety: Overview of Toxic Effects in Humans and Aquatic Animals

Luo

Xie

et al. 2020

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131

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Titanium dioxide nanoparticles (nano‐TiO2) are widely used in consumer products, raising environmental and health concerns. An overview of the toxic effects of nano‐TiO2 on human and environmental health is provided. A meta‐analysis is conducted to analyze the toxicity of nano‐TiO2 to the liver, circulatory system, and DNA in humans. To assess the environmental impacts of nano‐TiO2, aquatic environments that receive high nano‐TiO2 inputs are focused on, and the toxicity of nano‐TiO2 to aquatic organisms is discussed with regard to the present and predicted environmental concentrations. Genotoxicity, damage to membranes, inflammation and oxidative stress emerge as the main mechanisms of nano‐TiO2 toxicity. Furthermore, nano‐TiO2 can bind with free radicals and signal molecules, and interfere with the biochemical reactions on plasmalemma. At the higher organizational level, nano‐TiO2 toxicity is manifested as the negative effects on fitness‐related organismal traits including feeding, reproduction and immunity in aquatic organisms. Bibliometric analysis reveals two major research hot spots including the molecular mechanisms of toxicity of nano‐TiO2 and the combined effects of nano‐TiO2 and other environmental factors such as light and pH. The possible measures to reduce the harmful effects of nano‐TiO2 on humans and non‐target organisms has emerged as an underexplored topic requiring further investigation.

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Section: Meta-analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rethinking Nano‐TiO₂ Safety: Overview of Toxic Effects in Humans and Aquatic Animals

Luo

Xie

et al. 2020

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131

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“…Interactions of ROS with DNA and persistent epithelial injury can result in DNA damage and oxidative DNA-base modifications such as 8-Oxoguanine [90,93]. The formation of pre-neoplastic epithelial lesions, intestinal adenomas/carcinomas, or alterations in the size of tumors has been shown to be accompanied by downregulation of epidermal growth factor receptor (EGFR) and AKT, as well as downregulation of tumor suppressor genes e.g., p53 and p21 [129,149,150]. An up-and downregulation of p53 has been observed, which probably displayed different stages within the onset of adverse effects, triggered by E171 exposure.…”

Section: Mode Of Actionmentioning

confidence: 99%

“…Apaf-1 is a cytoplasmic protein initiating apoptosis. BAX is an apoptotic regulator, which binds with the BCL-2 gene to initiate apoptosis and is closely related to the p53 pathway and the formation of colon cancer [129,150]. Upregulation of the master regulator for lysosomal biogenesis and autophagy transcriptional factor EB (TfEB) as well as the increased expression of LC-3 and Beclin-1 (predictive markers for colorectal cancer) contribute to the assumption that the exposure to E171 and the resulting induction of persistent inflammation could be associated with an increased risk for colorectal cancer [128].…”

Section: Mode Of Actionmentioning

confidence: 99%

Possible Adverse Effects of Food Additive E171 (Titanium Dioxide) Related to Particle Specific Human Toxicity, Including the Immune System

Bischoff

Kok

Sijm

et al. 2020

IJMS

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Titanium dioxide (TiO2) is used as a food additive (E171) and can be found in sauces, icings, and chewing gums, as well as in personal care products such as toothpaste and pharmaceutical tablets. Along with the ubiquitous presence of TiO2 and recent insights into its potentially hazardous properties, there are concerns about its application in commercially available products. Especially the nano-sized particle fraction (<100 nm) of TiO2 warrants a more detailed evaluation of potential adverse health effects after ingestion. A workshop organized by the Dutch Office for Risk Assessment and Research (BuRO) identified uncertainties and knowledge gaps regarding the gastrointestinal absorption of TiO2, its distribution, the potential for accumulation, and induction of adverse health effects such as inflammation, DNA damage, and tumor promotion. This review aims to identify and evaluate recent toxicological studies on food-grade TiO2 and nano-sized TiO2 in ex-vivo, in-vitro, and in-vivo experiments along the gastrointestinal route, and to postulate an Adverse Outcome Pathway (AOP) following ingestion. Additionally, this review summarizes recommendations and outcomes of the expert meeting held by the BuRO in 2018, in order to contribute to the hazard identification and risk assessment process of ingested TiO2.

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“…A considerable amount of studies have also examined the influence of TiO 2 NPs on human cell lines in in vitro experiments. For the human keratinocyte cell line HaCaT (human adult low calcium high-temperature keratinocytes), following exposure to TiO 2 NPs, decreased cell viability and induction of the cell cycle arrest have been demonstrated [ 110 ]. Rutile TiO 2 NPs with <100 nm particle size were also tested on a human metastatic melanoma cell line, where a reduction in cell metabolic activity and cytotoxic response were observed.…”

Section: Routes Of Exposure and Toxicity Of Tio 2 mentioning

confidence: 99%

Titanium Dioxide Nanoparticles in Food and Personal Care Products—What Do We Know about Their Safety?

et al. 2020

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Titanium dioxide (TiO2) is a material of diverse applications commonly used as a food additive or cosmetic ingredient. Its prevalence in products of everyday use, especially in nanosize, raises concerns about safety. Current findings on the safety of titanium dioxide nanoparticles (TiO2 NPs) used as a food additive or a sunscreen compound are reviewed and systematized in this publication. Although some studies state that TiO2 NPs are not harmful to humans through ingestion or via dermal exposure, there is a considerable number of data that demonstrated their toxic effects in animal models. The final agreement on the safety of this nanomaterial has not yet been reached among researchers. There is also a lack of official, standardized guidelines for thorough characterization of TiO2 NPs in food and cosmetic products, provided by international authorities. Recent advances in the application of ‘green-synthesized’ TiO2 NPs, as well as comparative studies of the properties of ‘biogenic’ and ‘traditional’ nanoparticles, are presented. To conclude, perspectives and directions for further studies on the toxicity of TiO2 NPs are proposed.

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Combination of bioanalytical approaches and quantitative proteomics for the elucidation of the toxicity mechanisms associated to TiO2 nanoparticles exposure in human keratinocytes

Cited by 20 publications

References 45 publications

Rethinking Nano‐TiO₂ Safety: Overview of Toxic Effects in Humans and Aquatic Animals

Rethinking Nano‐TiO₂ Safety: Overview of Toxic Effects in Humans and Aquatic Animals

Possible Adverse Effects of Food Additive E171 (Titanium Dioxide) Related to Particle Specific Human Toxicity, Including the Immune System

Titanium Dioxide Nanoparticles in Food and Personal Care Products—What Do We Know about Their Safety?

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Combination of bioanalytical approaches and quantitative proteomics for the elucidation of the toxicity mechanisms associated to TiO2 nanoparticles exposure in human keratinocytes

Cited by 20 publications

References 45 publications

Rethinking Nano‐TiO2 Safety: Overview of Toxic Effects in Humans and Aquatic Animals

Rethinking Nano‐TiO2 Safety: Overview of Toxic Effects in Humans and Aquatic Animals

Possible Adverse Effects of Food Additive E171 (Titanium Dioxide) Related to Particle Specific Human Toxicity, Including the Immune System

Titanium Dioxide Nanoparticles in Food and Personal Care Products—What Do We Know about Their Safety?

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Rethinking Nano‐TiO₂ Safety: Overview of Toxic Effects in Humans and Aquatic Animals

Rethinking Nano‐TiO₂ Safety: Overview of Toxic Effects in Humans and Aquatic Animals