Combination of biotransformation and chromatography for the isolation and purification of mannoheptulose

Kappler-Tanudyaya, Nathalie; Schmitt, H.; Tippkötter, N.; Meyer, Lina; Lenzen, Sigurd; Ulber, Roland

doi:10.1002/biot.200700004

Cited by 3 publications

(1 citation statement)

References 12 publications

(12 reference statements)

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“…Whole avocado fruit of the Hass variety was obtained from an Australian commercial crop (De Paoli Farms, Queensland, Australia) in an unripe state and stored in a cold room under 5 • C. Within 24 h after the harvest, the whole fruit was washed with water, and the flesh and peel were commercially shredded by a disintegrator, before being transferred into a freeze-drying machine for 96 h. The mass percentage of MH in avocado extract (AvX) was 1.9%, which was determined by HPLC at Southern Cross University (T Block Level 3, Military Road, Lismore, NSW, Australia) according to the methods developed by Kappler-Tanudyaya et al [17] and Shaw et al [18]. The placebo was a commercially available precooked fine-ground corn meal (~37 kCal, PANCORN, USA) that was spiked with 5% spinach powder (Australian Spinach Vegetable Powder, Boost Nutrients, NSW, Australia).…”

Section: Supplement Preparationsmentioning

confidence: 99%

Effects of an Unripe Avocado Extract on Glycaemic Control in Individuals with Obesity: A Double-Blinded, Parallel, Randomised Clinical Trial

Zhao,

Ingram,

Gumpricht

et al. 2023

Nutrients

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Background: Unripe avocados (Persea americana) are naturally enriched in mannoheptulose (MH), which is a candidate caloric restriction mimetic. Objectives: To evaluate the effects of a diet supplement made from unripe avocado on glucose tolerance, and cardiometabolic risk factors in free-living nondiabetic adults with obesity. Methods: In a double-blinded, randomised controlled trial, 60 adults (female n = 47, age 48 ± 13 years, BMI 34.0 ± 2.6 kg/m2) were stratified by sex and randomised to avocado extract (AvX, 10 g finely ground, freeze-dried unripe avocado) or placebo (10 g finely ground cornmeal plus 5% spinach powder) daily, for 12 weeks. The primary outcome was a change in glucose area under the curve (AUC) in response to a 75 g oral glucose tolerance test. A post-hoc analysis was subsequently performed in a subgroup with insulin AUC above the median of baseline values after removal of participants >2 SD from the mean. Results: There were no between-group differences in glucose AUC (p = 0.678), insulin AUC (p = 0.091), or cardiovascular outcomes. In the subgroup analysis, insulin AUC was lower in AxV versus placebo (p = 0.024). Conclusions: Daily consumption of unripe avocado extract enriched in MH did not alter glucose tolerance or insulin sensitivity in nondiabetic adults with obesity, but the data provided preliminary evidence for a benefit in insulin AUC in a subgroup of participants with elevated baseline postprandial insulin levels.

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Section: Supplement Preparationsmentioning

confidence: 99%

Effects of an Unripe Avocado Extract on Glycaemic Control in Individuals with Obesity: A Double-Blinded, Parallel, Randomised Clinical Trial

Zhao,

Ingram,

Gumpricht

et al. 2023

Nutrients

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Additive activation of glucokinase by the bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase and the chemical activator LY2121260

Baltrusch

Schmitt

Brix

et al. 2012

Biochemical Pharmacology

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The locks and keys to industrial biotechnology

Wohlgemuth¹

2009

New Biotechnology

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The sustainable use of resources by Nature to synthesize the required products at the right place, when they are needed, continues to be the role model for total synthesis and production in general. The combination of molecular and engineering science and technology in the biotechnological approach needs no protecting groups at all and has therefore been established for numerous large-scale routes to both natural and synthetic products in industry. The use of biobased raw materials for chemical synthesis, and the economy of molecular transformations like atom economy and step economy are of growing importance. As safety, health and environmental issues are key drivers for process improvements in the chemical industry, the development of biocatalytic reactions or pathways replacing hazardous reagents is a major focus. The integration of the biocatalytic reaction and downstream processing with product isolation has led to a variety of in situ product recovery techniques and has found numerous successful applications. With the growing collection of biocatalytic reactions, the retrosynthetic thinking can be applied to biocatalysis as well. The introduction of biocatalytic reactions is uniquely suited to cost reductions and higher quality products, as well as to more sustainable processes. The transfer of Nature's simple and robust sensing and control principles as well as its reaction and separation organization into useful technical systems can be applied to different fermentations, biotransformations and downstream processes. Biocatalyst and pathway discovery and development is the key towards new synthetic transformations in industrial biotechnology.

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Combination of biotransformation and chromatography for the isolation and purification of mannoheptulose

Cited by 3 publications

References 12 publications

Effects of an Unripe Avocado Extract on Glycaemic Control in Individuals with Obesity: A Double-Blinded, Parallel, Randomised Clinical Trial

Effects of an Unripe Avocado Extract on Glycaemic Control in Individuals with Obesity: A Double-Blinded, Parallel, Randomised Clinical Trial

Additive activation of glucokinase by the bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase and the chemical activator LY2121260

The locks and keys to industrial biotechnology

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