Combination of Cefditoren and
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            -acetyl-
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            -Cysteine Shows a Synergistic Effect against Multidrug-Resistant Streptococcus pneumoniae Biofilms

Llamosí, Mirella; Sempere, Julio; Coronel, Pilar; Gimeno, Mercedes; Yuste, José; Domenech, Mirian

doi:10.1128/spectrum.03415-22

Cited by 4 publications

(2 citation statements)

References 53 publications

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“…Other approaches to combat biofilms include utilising non-conventional agents in conjunction with antibiotics. Results of a study done by Llamosí et al 26 revealed that when an antioxidant N-acetyl-L-cysteine (NAC) was used in combination with cefditoren, a cephalosporin antibiotic, NAC significantly enhanced inhibition and eradication of MDR S. pneumoniae biofilms. Similarly, enhanced anti-biofilm effects were seen when nitric oxide was utilised synergistically with antibiotics.…”

Section: Enhancing Activity Of Conventional Antimicrobials Synergisti...mentioning

confidence: 99%

Chronic Obstructive Pulmonary Disease: Biofilm Mediated Exacerbation and Innovative Therapeutic Approaches

Berger,

Kagan,

Bock

et al. 2023

EMJ Respir

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Chronic obstructive pulmonary disease (COPD) is a leading cause of death. The prevalence of the disease and associated mortality continue to increase. Bacterial and viral infections are responsible for the transition of the disease to more severe stages, resulting in COPD exacerbation. Biofilms, communities of micro-organisms that contribute to COPD exacerbation, pose a formidable challenge for effective pharmacotherapy. This review focuses on the development of biofilms, and approaches to inhibit and eradicate biofilms.

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Section: Enhancing Activity Of Conventional Antimicrobials Synergisti...mentioning

confidence: 99%

Chronic Obstructive Pulmonary Disease: Biofilm Mediated Exacerbation and Innovative Therapeutic Approaches

Berger,

Kagan,

Bock

et al. 2023

EMJ Respir

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“…Propolis has antimicrobial activity against planktonic forms as well as an inhibitory effect on the biofilm production of Staphylococcus aureus (both methicillin-susceptible (MS) and methicillin-resistant (MR), Staphylococcus epidermidis, and Pseudomonas aeruginosa [15][16][17]. Both NAC and propolis showed synergistic effects with certain antibiotics when tested as a single compound (propolis with beta-lactam antibiotics and NAC with cephalosporin) demonstrating the ability to enhance their effects even against resistant strains, which is a promising and effective strategy against bacterial biofilms [18,19]. However, the synergistic effect of a fixed combination of NAC and dry propolis extract against bacterial biofilms has not yet been studied.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Antibiofilm Effect of N-Acetyl-L-cysteine/Dry Propolis Extract Combination on Bacterial Pathogens Isolated from Upper Respiratory Tract Infections

Božić,

Ćirković,

Milovanović

et al. 2023

Pharmaceuticals

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Bacterial biofilms play an important role in the pathogenesis of chronic upper respiratory tract infections. In addition to conventional antimicrobial therapy, N-acetyl-L-cysteine (NAC) and propolis are dietary supplements that are often recommended as supportive therapy for upper respiratory tract infections. However, no data on the beneficial effect of their combination against bacterial biofilms can be found in the scientific literature. Therefore, the aim of our study was to investigate the in vitro effect of N-acetyl-L-cysteine (NAC) and dry propolis extract in fixed combinations (NAC/dry propolis extract fixed combination) on biofilm formation by bacterial species isolated from patients with chronic rhinosinusitis, chronic otitis media, and chronic adenoiditis. The prospective study included 48 adults with chronic rhinosinusitis, 29 adults with chronic otitis media, and 33 children with chronic adenoiditis. Bacteria were isolated from tissue samples obtained intraoperatively and identified using the MALDI-TOF Vitek MS System. The antimicrobial activity, synergism, and antibiofilm effect of NAC/dry propolis extract fixed combination were studied in vitro. A total of 116 different strains were isolated from the tissue samples, with staphylococci being the most frequently isolated in all patients (57.8%). MICs of the NAC/dry propolis extract fixed combination ranged from 1.25/0.125 to 20/2 mg NAC/mg propolis. A synergistic effect (FICI ≤ 0.5) was observed in 51.7% of strains. The majority of isolates from patients with chronic otitis media were moderate biofilm producers and in chronic adenoiditis they were weak biofilm producers, while the same number of isolates in patients with chronic rhinosinusitis were weak and moderate biofilm producers. Subinhibitory concentrations of the NAC/propolis combination ranging from 0.625–0.156 mg/mL to 10–2.5 mg/mL of NAC combined with 0.062–0.016 mg/mL to 1–0.25 mg/mL of propolis inhibited biofilm formation in all bacterial strains. Suprainhibitory concentrations ranging from 2.5–10 mg/mL to 40–160 mg/mL of NAC in combination with 0.25–1 mg/mL to 4–16 mg/mL of propolis completely eradicated the biofilm. In conclusion, the fixed combination of NAC and dry propolis extract has a synergistic effect on all stages of biofilm formation and eradication of the formed biofilm in bacteria isolated from upper respiratory tract infections.

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PCV13 vaccine prevents pneumococcal biofilms without affecting Staphylococcus aureus population within the polymicrobial biofilm

Sempere,

Yuste,

Domenech

2024

Front. Immunol.

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In respiratory pathogens such as Streptococcus pneumoniae, biofilm formation is associated with the colonization of the nasopharynx and chronic respiratory infection. Previous data have shown that pneumococcal conjugate vaccines (PCVs) had an impact on S. pneumoniae colonization and a potential replacement by other respiratory pathogens such as Staphylococcus aureus. The objective of this work was to evaluate the evasion of the immune system by monospecific biofilms and by S. aureus-S. pneumoniae mixed biofilms. We performed opsonophagocytosis assays (OPA) using human HL-60 against previously disaggregated monospecific biofilms of MSSA, MRSA and S. aureus-S. pneumoniae mixed biofilms. We used pre-immune and post-immune serum from immunocompetent adult patients vaccinated with PCV13. Immune sera had a clear effect in reducing pneumococcal biofilms of serotypes 3, 14, 18C, 19F and 19A, whereas had no effect in non-PCV13 serotypes such as 8, 11A and 24F. Our study confirmed that serum from vaccinated patients with PCV13 did not have any effect in reducing S. aureus population in monospecific biofilms, regardless the methicillin resistance phenotype. Moreover, immunized sera from vaccinated patients with PCV13 did not have any effect in S. aureus population in the mixed biofilm, whereas significantly reduced the population of pneumococcal serotype 19A strain in the mixed biofilm which is of great interest because this serotype is included in PCV13, and it is associated with vaccine failures.

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Combination of Cefditoren and N -acetyl- l -Cysteine Shows a Synergistic Effect against Multidrug-Resistant Streptococcus pneumoniae Biofilms

Cited by 4 publications

References 53 publications

Chronic Obstructive Pulmonary Disease: Biofilm Mediated Exacerbation and Innovative Therapeutic Approaches

Chronic Obstructive Pulmonary Disease: Biofilm Mediated Exacerbation and Innovative Therapeutic Approaches

In Vitro Antibiofilm Effect of N-Acetyl-L-cysteine/Dry Propolis Extract Combination on Bacterial Pathogens Isolated from Upper Respiratory Tract Infections

PCV13 vaccine prevents pneumococcal biofilms without affecting Staphylococcus aureus population within the polymicrobial biofilm

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