Combination of Chlorhexidine and Silver Nanoparticles: an Efficient Wound Infection and Healing Control System

Myronov, P. F.; Sulaieva, Oksana; Korniienko, Viktoriia; Banasiuk, Rafał; Vielikov, M.; Husak, Ye.; Pernakov, Mykola; Deineka, Volodymyr; Yusupova, A. A.; Hristova, M.-T.; Savchenko, A.; Holubnycha, Viktoriia Mykolaivna; Pogorielov, Мaksym

doi:10.1007/s12668-021-00834-5

Cited by 8 publications

(5 citation statements)

References 30 publications

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“…electronics, optics, catalysis, food, health and environment [ 19 ]. In packaging [ 20 ] textile coatings [ 21 ], wound dressings [ 22 , 23 ], cleaning products [ 24 ], cosmetics [ 25 ], pharmaceutical gel [ 26 ], and against skin diseases[ 25 ] silver nanoparticles are often employed.…”

Section: Introductionmentioning

confidence: 99%

Phytonano silver for cosmetic formulation- synthesis, characterization, and assessment of antimicrobial and antityrosinase potential

George,

Devi

2024

Discover Nano

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Novel formulations of silver nanoparticles remain exciting if it is applicable for cosmetic purposes. This study proposes a value-added brand-new nanomaterial for improving skin complexion by inhibiting melanin development. This work aims to develop cost effective, efficient, natural silver nanoparticles phytomediated by aqueous extract of leaf sheath scales of Cocos nucifera (Cn-AgNPs) having potential as tyrosinase inhibitors hindering melanin synthesis. The formation of Cn-AgNPs was assessed spectrophotometrically and confirmed by the sharp SPR spectrum at 425 nm. The chemical composition profiling was characterized by X-ray diffraction (XRD) and Fourier Transform Infrared (FTIR) spectroscopy. The morphology was confirmed by Field Emission Scanning Electron Microscopy (FESEM) and the thermal stability was assessed by Thermogravimetric analysis (TGA). Pharmacological application studies supported the materialization of Cn-AgNPs with significant antityrosinase potential and considerably improved antibacterial and antioxidant properties. Cn-AgNPs showed potential antibacterial effects against gram-positive and negative strains, including prominent infectious agents of the skin. Antioxidant capacity was confirmed with an IC50 of 57.8 μg/mL by DPPH radical scavenging assay. Furthermore, in vitro melanin content determination was performed using SK-MEL cells. Cell line studies proved that Cn-AgNPs decrease the melanin content of cells. The IC50 value obtained was 84.82 μg/mL. Hence Cn-AgNPs is proposed to be acting as a whitening agent through lessening cellular melanin content and as a significant inhibitor of tyrosinase activity. The antioxidant properties and antibacterial effects can contribute to skin rejuvenation and can prevent skin infections as well. This evidence proposes the development of a new nanostructured pharmaceutical and cosmetic formulation from Cocos nucifera leaf sheath scales.

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Section: Introductionmentioning

confidence: 99%

Phytonano silver for cosmetic formulation- synthesis, characterization, and assessment of antimicrobial and antityrosinase potential

George,

Devi

2024

Discover Nano

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“…Some interesting findings were reported by Zhou et al, who prepared a composite of poly(L-lactide) microcapsules of chlorhexidine with superficially adsorbed silver nanoparticles and found better antibacterial properties against E. coli and S. aureus with less usage of silver, compared to silver nanoparticles carrying non-drug loaded microcapsules [ 1 ]. Elsewhere, Myronov et al reported that adding silver nanoparticles to chlorhexidine treatment improved infected wound healing through the acceleration of bacteria elimination ( E. coli , P. aeruginosa , and S. aureus ) and M2 macrophage polarization [ 2 ]. Pernakov et al established the accelerated wound healing effect of silver nano-architectures and chlorhexidine when combined, compared with single treatments with both agents, but did not observe any synergism in the antibacterial activity against E. coli and P. aeruginosa [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Chlorhexidine–Silver Nanoparticle Conjugation Leading to Antimicrobial Synergism but Enhanced Cytotoxicity

Ivanova,

Ermenlieva,

Simeonova

et al. 2023

Pharmaceutics

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This study explored the potential synergism within chlorhexidine–silver nanoparticle conjugates against Influenza type A, Staphylococcus aureus, Escherichia coli, and Candida albicans. Silver nanoparticles (SN) were obtained by the reduction of silver ions with green tea total phenolic extract and conjugated with chlorhexidine (Cx). The particles were characterized by UV-Vis and FTIR spectroscopies, dynamic light scattering, X-ray diffraction, and transmission electron microscopy. A stable negatively charged nano-silver colloid (ζ = −50.01) was obtained with an average hydrodynamic diameter of 92.34 nm. In the presence of chlorhexidine, the spectral data and the shift of the zeta potential to positive values (ζ = +44.59) revealed the successful sorption of the drug onto the silver surface. The conjugates (SN-Cx) demonstrated potentiation in their effects against S. aureus and C. albicans and synergism against E. coli with minimal inhibitory concentrations of SN at 5.5 µg/mL + Cx 8.8 µg/mL. The SN showed excellent virucidal properties, increasing with time, and demonstrated low toxicity. However, the coupling of the cationic chlorhexidine with nano-silver did not reduce its intrinsic cytotoxicity on various cell lines (MDCK, BJ, and A549). The newly synthesized antimicrobial agent exhibited an extended and promising therapeutic spectrum and needs to be further evaluated regarding the designated route of administration in three-dimensional cell models (e.g., nasal, bronchial, dermal, ocular, etc.).

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“…Both agents are widely studied and well-described in literature to possess distinct antimicrobial properties, which made a lot of scientists curious about a possible synergism, additive effects, or potentiation of their activities. Some interesting findings so far are of Zhou et al who prepared a composite of poly(L-lactide) microcapsules of chlorhexidine with superficially adsorbed silver nanoparticles and found better antibacterial properties against E. coli and S. aureus with less usage of silver as compared to silver nanoparticles-carrying non-drug loaded microcapsules [1]; Myronov et al reported that adding silver nanoparticles to chlorhexidine treatment improves infected wound healing through acceleration of bacteria elimination (E. coli, P. aeruginosa, 2 S. aureus) and M2 macrophage polarization [2]; Pernakov et al established accelerated would healing effect of silver nano-architectures and chlorhexidine, when combined, as compared with the single treatments with both agents, but did not observe any synergism in the antibacterial activity against E. coli and P. aeruginosa [3]. Other authors reported synergism or potentiation of SN and Cx against Candida spp.…”

Section: Introductionmentioning

confidence: 99%

Chlorhexidine-Silver Nanoparticles Conjugation Leading to Antimicrobial Synergism but highly increased cytotoxicity

Ivanova,

Ermenlieva,

Simeonova

et al. 2023

Preprint

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This study explored the potential synergism within chlorhexidine-silver nanoparticles conjugates against Influenza type A, Staphylococcus aureus, Escherichia coli, and Candida albicans. Silver nanoparticles (SN) were obtained by the reduction of silver ions with green tea total phenolic extract and conjugated with chlorhexidine (Cx). The particles were characterized by UV-Vis and FTIR spectroscopies, dynamic light scattering, X-ray diffraction, and transmission electron microscopy. A stable negatively-charged nano-silver colloid (ζ=-50.01) was obtained with an average hydrodynamic diameter of 92.34 nm. In the presence of chlorhexidine, the spectral data and the shift of the zeta potential to positive values (ζ=+44.59) revealed the successful sorption of the drug onto the silver surface. The conjugates (SN-Cx) demonstrated potentiation in the effects against S. aureus and C. albicans, and synergism against E. coli with minimal inhibitory concentrations of SN 5.5 µg/mL+Cx 8.8 µg/mL. The SN showed excellent, increasing with time, virucidal properties, and low toxicity. The coupling of the cationic chlorhexidine with nano-silver, however, did not reduce its intrinsic cytotoxicity on various cell lines (MDCK, BJ, and А549). The newly-synthesized antimicrobial agent presented with an extended and promising therapeutic spectrum and needs to be further evaluated on relevant to the designated route of administration three-dimensional cell models (e.g., nasal, bronchial, dermal, ocular, etc.).

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Combination of Chlorhexidine and Silver Nanoparticles: an Efficient Wound Infection and Healing Control System

Cited by 8 publications

References 30 publications

Phytonano silver for cosmetic formulation- synthesis, characterization, and assessment of antimicrobial and antityrosinase potential

Phytonano silver for cosmetic formulation- synthesis, characterization, and assessment of antimicrobial and antityrosinase potential

Chlorhexidine–Silver Nanoparticle Conjugation Leading to Antimicrobial Synergism but Enhanced Cytotoxicity

Chlorhexidine-Silver Nanoparticles Conjugation Leading to Antimicrobial Synergism but highly increased cytotoxicity

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