Combination of cold plasma and pulsed electric fields – A rationale for cancer patients in palliative care

Wolff, Christina M.; Stoffels, Ingo; Woedtke, Thomas von; Weltmann, Klaus‐Dieter; Bekeschus, Sander; Kolb, Juergen F.

doi:10.1016/j.cpme.2020.100096

Cited by 20 publications

(13 citation statements)

References 182 publications

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“…Moreover, lymphomas are still cancer cells that share several driver mutations with those found in solid tumors. Nevertheless, there are challenges with the applicability of the concept of using PEF and plasma in clinical therapy, which we have outlined previously [25]. Among those are, for example, in melanoma, the location of many metastases beneath the skin in the stratum corneum, which are difficult to reach directly with plasmas.…”

Section: Discussionmentioning

confidence: 99%

“…The supplementation with either antioxidant, a pan-caspase-inhibitor or a ferroptosis inhibitor, all partially rescued lymphoma cells from terminal cell death, which contributes to the mechanistic understanding of this combination treatment.Cancers 2020, 12, 845 2 of 20 mitochondrial/intrinsic to receptor/extrinsic pathways as well as caspase-or Bcl-2 (B-cell lymphoma 2)-independent mechanisms [21][22][23].Pulsed electric fields in the microsecond range (µsPEFs) are used in electrochemotherapy [24]. Application of µsPEF is an accredited anticancer therapy in the clinics [25]. µsPEFs transiently compromise the plasma cell membrane integrity of the cancer cells, allowing the chemotherapeutics, e.g., bleomycin and cisplatin, to enter the cell [26,27].…”

mentioning

confidence: 99%

“…Pulsed electric fields in the microsecond range (µsPEFs) are used in electrochemotherapy [24]. Application of µsPEF is an accredited anticancer therapy in the clinics [25]. µsPEFs transiently compromise the plasma cell membrane integrity of the cancer cells, allowing the chemotherapeutics, e.g., bleomycin and cisplatin, to enter the cell [26,27].…”

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confidence: 99%

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Combination Treatment with Cold Physical Plasma and Pulsed Electric Fields Augments ROS Production and Cytotoxicity in Lymphoma

Wolff

Kolb

Weltmann

et al. 2020

Cancers

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New approaches in oncotherapy rely on the combination of different treatments to enhance the efficacy of established monotherapies. Pulsed electric fields (PEFs) are an established method (electrochemotherapy) for enhancing cellular drug uptake while cold physical plasma is an emerging and promising anticancer technology. This study aimed to combine both technologies to elucidate their cytotoxic potential as well as the underlying mechanisms of the effects observed. An electric field generator (0.9–1.0 kV/cm and 100-μs pulse duration) and an atmospheric pressure argon plasma jet were employed for the treatment of lymphoma cell lines as a model system. PEF but not plasma treatment induced cell membrane permeabilization. Additive cytotoxicity was observed for the metabolic activity and viability of the cells while the sequence of treatment in the combination played only a minor role. Intriguingly, a parallel combination was more effective compared to a 15-min pause between both treatment regimens. A combination effect was also found for lipid peroxidation; however, none could be observed in the cytosolic and mitochondrial reactive oxygen species (ROS) production. The supplementation with either antioxidant, a pan-caspase-inhibitor or a ferroptosis inhibitor, all partially rescued lymphoma cells from terminal cell death, which contributes to the mechanistic understanding of this combination treatment.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Combination Treatment with Cold Physical Plasma and Pulsed Electric Fields Augments ROS Production and Cytotoxicity in Lymphoma

Wolff

Kolb

Weltmann

et al. 2020

Cancers

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“…The plasma chemistry -particularly the production of reactive species-is expected to differ from a typical APPJs which should open new perspectives of applications. For instance, the EASII-jet configuration offers the opportunity of local production of reactive species with intense pulsed EF desired to trigger biological mechanisms to support alternative cancer therapies 6 . The cheap and simple design of EASII-jet can also be implemented to integrated microfluidic systems to active liquids and surfaces 9 .…”

Section: Discussionmentioning

confidence: 99%

“…Many applications rely on this plasma reactivity in catalysis and materials science processes 3,4 . More generally, APPJs are involved in a large panel of applications such as in medicine to stimulate biological responses 5,6 , agriculture 7 and liquid activation 8,9 . Beyond the non-equilibrium reactive chemistry, APPJs generate guided ionization waves 10 (IWs) -guided streamers 11 -driven by a high electric field (EF) ahead of the discharge channel 12,13 .…”

Section: Introductionmentioning

confidence: 99%

Electrodeless atmospheric secondary induced ionization jet (EASII-jet): Dynamics and properties of a transferred helium plasma source

et al. 2020

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EP -This paper was selected as an Editor's Pick. This work introduces a versatile electrodeless cold plasma source in Helium based on an atmospheric pressure plasma jet (APPJ) device. The latter is used to generate guided ionization waves (IWs) impinging on a dielectric tube carrying He gas for the production of secondary induced ionization wave (SIIW). The dynamics of the plasma transfer across the dielectric is investigated by time resolved iCCD imaging technique and electrical diagnostics. Three operating modes are reported depending on the high voltage (HV) pulse duration which enables either a single or a double SIIW per HV pulse. Properties of the IWs and the double SIIW are reported. The gas temperature profiles of the plasma plumes expanding in to the ambient is investigated by optical emission spectroscopy. The results will be correlated to the time evolution of the current. This electrodeless atmospheric secondary induced ionization jet (EASII-jet) configuration can be easily implemented to electrically sensitive systems particularly in the fields of plasma medicine, pulsed electric field therapies and plasma activated liquids.

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How Does Cold Plasma Work in Medicine?

Bekeschus

Woedtke

Schmidt

2022

Textbook of Good Clinical Practice in Cold Plasma Therapy

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Combination of cold plasma and pulsed electric fields – A rationale for cancer patients in palliative care

Cited by 20 publications

References 182 publications

Combination Treatment with Cold Physical Plasma and Pulsed Electric Fields Augments ROS Production and Cytotoxicity in Lymphoma

Combination Treatment with Cold Physical Plasma and Pulsed Electric Fields Augments ROS Production and Cytotoxicity in Lymphoma

Electrodeless atmospheric secondary induced ionization jet (EASII-jet): Dynamics and properties of a transferred helium plasma source

How Does Cold Plasma Work in Medicine?

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