1992
DOI: 10.1620/tjem.168.1
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Combination of Conventional and Endotoxin-Specific Limulus Tests for Measurement of Polysaccharides in Sera of Rabbits with Experimental Systemic Candidiasis.

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Cited by 10 publications
(7 citation statements)
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“…Ikegami et al (4) reported a rise in the fungal index in patients with candidiasis. Results from our laboratory confirmed that differences between titers measured by the two tests were higher in culture supernatants of C. albicans (12), plasma of rabbits with systemic candidiasis (12), and plasma of patients with candidemia (3).…”
Section: Discussionsupporting
confidence: 61%
“…Ikegami et al (4) reported a rise in the fungal index in patients with candidiasis. Results from our laboratory confirmed that differences between titers measured by the two tests were higher in culture supernatants of C. albicans (12), plasma of rabbits with systemic candidiasis (12), and plasma of patients with candidemia (3).…”
Section: Discussionsupporting
confidence: 61%
“…Thus, the difference in titers between the conventional Limulus test and the endotoxin-specific test is a useful, although indirect, indicator for detecting (133)-␤-Dglucan. We have reported previously that the conventional Limulus test reacts with both curdlan [linear (133)-␤-D-glucan] and culture supernatants of Candida albicans (15). We also demonstrated that the difference in titers between the two Limulus tests, termed the fungal index (6), increases in a rabbit model of systemic candidiasis (15) and in patients with candidiasis (10).…”
mentioning
confidence: 72%
“…As shown in the present study, factor G reacted with fungal cell suspension, and the conventional limulus test was useless to demonstrate contamination with endotoxin in yeast cell suspension. To improve the specificity of the conventional limulus test against endotoxin, Obayashi et al (5,6) developed an endotoxin-specific assay devoid of the limulus factor G. The endotoxin-specific chromogenic limulus test reacts only with endotoxin, and not with (1+3)-P-D-glucan (5,6) or culture supernatants of C. albicans (3). Therefore, the endotoxin-specific limulus test is the only method that can verify contamination with endotoxin in yeast cell suspensions.…”
Section: Discussionmentioning
confidence: 99%