Combination of different substrates to improve anaerobic digestion of sewage sludge in a wastewater treatment plant

Park, Ki Young; Jang, Hyun Min; Park, Mee-Rye; Lee, Kwanyong; Kim, Daegi; Kim, Young Mo

doi:10.1016/j.ibiod.2016.01.006

Cited by 59 publications

(22 citation statements)

References 13 publications

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“…2d, the pH was approximately 7.5 in the H test on day 10, which was beneficial for Sedimentibacter metabolism. The other dominant genera in H were Cloacibacillus (class Synergistia , 3%) and Acetoanaerobium (class Bacilli , 6%), which were specialized in amino-acid- biodegradation [48] and HAc production (from H 2 and CO 2 ) [49]. The dominant genera in the control included Thauera (2%), Nitrosospira (1%), Longilinea (5%) and Levilinea (4%), which were all common microbial consortia during the wastewater treatment.…”

Section: Resultsmentioning

confidence: 99%

What could the entire cornstover contribute to the enhancement of waste activated sludge acidification? Performance assessment and microbial community analysis

Zhou

Zhang

Wen

et al. 2016

Biotechnol Biofuels

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BackgroundVolatile fatty acids (VFAs) production from waste activated sludge (WAS) digestion is constrained by unbalanced nutrient composition (low carbon-to-nitrogen ratio). Characteristics conditioning by extra carbon sources, normally in the mixture of raw solid, has been reported to be an efficient approach to enhance WAS acidification. However, little attention has been paid to the contributions of other adjustment forms. Moreover, the corresponding ecological estimation has not been investigated yet.ResultsIn this study, the feasibility of corn stover (CS) conditioning with three adjustment forms [pretreated straw (S), hydrolysate (H) and hydrolysate + straw (HS)] in improving VFAs production from WAS was demonstrated. It was observed that the highest VFAs yield was achieved in H co-digesting test (574 mg COD/g VSS), while it was only 392 mg COD/g VSS for WAS digesting alone. VFAs composition was strongly adjustment form-dependent, as more acetic (HAc) and propionic (HPr) acids were generated in CS_HS and S, respectively. High-throughput sequencing analysis illustrated that acid (especially HAc)-producing characteristic genera (Bacteroides, Proteiniclasticum and Fluviicola) and HPr-producing characteristic genera (Mangroviflexus and Paludibacter) were detected by CS_HS and S conditioning, respectively.ConclusionsCorn stover conditioning greatly upgraded the WAS acidification performance, especially for the CS_H adjustment form, and the VFAs yield gained was considerably larger than that previously reported. CS adjustment forms played an important role in structuring the innate microbial community in WAS. Canonical correlation analysis illustrated that characteristic genera, with better hydrolysis and acidification abilities, could be enriched by the feedstocks with certain content of cellulose, hemicellulose or their saccharification hydrolysates. Moreover, ecological estimation revealed that, as far as the entire CS (including S and H) per acre was concerned, the capacity of WAS treatment would reach that produced in a one million mts capacity wastewater treatment plants (WWTPs) per day. These findings may have crucial implications for the operation of WWTPs.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-016-0659-y) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

What could the entire cornstover contribute to the enhancement of waste activated sludge acidification? Performance assessment and microbial community analysis

Zhou

Zhang

Wen

et al. 2016

Biotechnol Biofuels

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“…The adequate method should be primarily selected for the type of extracted DNA and the specificity of sample matrix. To our knowledge, commercial kits designed especially for DNA isolation from wastewater or AS samples are not available, hence why kits for soil or fecal samples are used in this type of research [ 45 , 46 , 47 , 48 ]. WWTP samples as a multi-component research material require an individual approach in choosing the optimal method of DNA extraction.…”

Section: Resultsmentioning

confidence: 99%

Identification of Selected Antibiotic Resistance Genes in Two Different Wastewater Treatment Plant Systems in Poland: A Preliminary Study

et al. 2020

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Antibiotic resistance is a growing problem worldwide. The emergence and rapid spread of antibiotic resistance determinants have led to an increasing concern about the potential environmental and public health endangering. Wastewater treatment plants (WWTPs) play an important role in this phenomenon since antibacterial drugs introduced into wastewater can exert a selection pressure on antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs). Therefore, WWTPs are perceived as the main sources of antibiotics, ARB and ARG spread in various environmental components. Furthermore, technological processes used in WWTPs and its exploitation conditions may influence the effectiveness of antibiotic resistance determinants’ elimination. The main aim of the present study was to compare the occurrence of selected tetracycline and sulfonamide resistance genes in raw influent and final effluent samples from two WWTPs different in terms of size and applied biological wastewater treatment processes (conventional activated sludge (AS)-based and combining a conventional AS-based method with constructed wetlands (CWs)). All 13 selected ARGs were detected in raw influent and final effluent samples from both WWTPs. Significant ARG enrichment, especially for tet(B, K, L, O) and sulIII genes, was observed in conventional WWTP. The obtained data did not show a clear trend in seasonal fluctuations in the abundance of selected resistance genes in wastewaters.

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“…Moreover, the proportions of Proteocatella and Proteiniclasticum were slightly higher in the TA pretreated samples than those in the AA samples ( Table S2 ). The other dominant genera for L-conditioning tests were Ethanoligenens (class Clostridia , ~2.3%) and Acetoanaerobium (class Bacilli , ~5.3%), which can, respectively, ferment several kinds of mono-, di- and oligosaccharides to form HAc 42 and produce HAc from H 2 and CO 2 43 . It should be noted that Acidaminobacter, Saccharofermentans, Papillibacter, Clostridium IV and Oscillibacter (belonging to class Clostrdia ) had totally different variance trends with the genera just mentioned above, being abundant only in the AA-S and TA-S. Clostridium IV was involved in the VFAs production and has been reported to produce cellulases, lipases, proteases, and other extracellular enzymes 44 .…”

Section: Resultsmentioning

confidence: 99%

Characterization of biocarbon-source recovery and microbial community shifts from waste activated sludge by conditioning with cornstover: Assessment of cellulosic compositions

Wen

Zhou

Zhang

et al. 2017

Sci Rep

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Most studies on the production of volatile fatty acids (VFAs) from waste activated sludge (WAS) digestion have focused on operating conditions, pretreatments and characteristic adjustments. Conditioning by extra carbon sources (ECS), normally added in a solid form, has been reported to be an efficient approach. However, this has caused considerable waste of monomeric sugars in the hydrolysate. In this study, the effects of two added forms (pretreated straw (S) and hydrolyzed liquid (L)) of cornstover (CS) on WAS acidification were investigated. To obtain different cellulosic compositions of CS, low-thermal or autoclaved assisted alkaline (TA or AA) pretreatments were conducted. The results showed that AA-L test achieved the highest VFAs value (653 mg COD/g VSS), followed by AA-S (613 mg COD/g VSS). These values were 12% and 28% higher, respectively, than that obtained in the TA-L and TA-S tests. Meanwhile, higher percentages of acetic acid were observed after AA pretreatment (~62% versus ~53% in TA). The added forms of CS played an important role in structuring the innate microbial community in the WAS, as shown by high-throughput sequencing and canonical correspondence analysis. The findings obtained in this work may provide a scientific basis for the potential implementation of co-digesting WAS with ECS simultaneously obtaining energy and high value-added products.

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Combination of different substrates to improve anaerobic digestion of sewage sludge in a wastewater treatment plant

Cited by 59 publications

References 13 publications

What could the entire cornstover contribute to the enhancement of waste activated sludge acidification? Performance assessment and microbial community analysis

What could the entire cornstover contribute to the enhancement of waste activated sludge acidification? Performance assessment and microbial community analysis

Identification of Selected Antibiotic Resistance Genes in Two Different Wastewater Treatment Plant Systems in Poland: A Preliminary Study

Characterization of biocarbon-source recovery and microbial community shifts from waste activated sludge by conditioning with cornstover: Assessment of cellulosic compositions

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