Combination of doxorubicin-based chemotherapy and polyethylenimine/p53 gene therapy for the treatment of lung cancer using porous PLGA microparticles

Shi, Xinjian; Li, Chunjie; Gao, Shuang; Zhang, Lingfei; Han, Haobo; Zhang, Jianxu; Shi, Wei; Li, Quanshun

doi:10.1016/j.colsurfb.2014.07.020

Cited by 46 publications

(36 citation statements)

References 28 publications

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“…The morphology of pDNA-NPs is smooth and spherical as confirmed by SEM and AFM images as shown in Figure 4, with particles size in the range of 200-250 nm. Spherical morphology is congruent by other observations reported in literature for a similar system [13,28,61].…”

Section: Pdna-np Tdl (%)supporting

confidence: 82%

“…Benfer et al reported encapsulating plasmid using PEG-PEI polyplexes with PLGA with encapsulation efficiencies of 26.4%-27.8% [11]. Shi et al obtained a drug load in the range of 0.71%-0.77% which led to 88%-92% encapsulation efficiency when encapsulating a combination of cancer medication and plasmid DNA in porous PLGA nanoparticles and as of plasmid only, load was 0.036%-0.041% with plasmid encapsulation efficiency of 36%-40% [61]. It is noticeable that, higher efficiencies can be achieved during encapsulating medication in comparison with plasmid DNA, as negative charge of plasmid increases its encapsulation efficiency in PEI 25K [11,61].…”

Section: Pdna-np Tdl (%)mentioning

confidence: 99%

“…Shi et al obtained a drug load in the range of 0.71%-0.77% which led to 88%-92% encapsulation efficiency when encapsulating a combination of cancer medication and plasmid DNA in porous PLGA nanoparticles and as of plasmid only, load was 0.036%-0.041% with plasmid encapsulation efficiency of 36%-40% [61]. It is noticeable that, higher efficiencies can be achieved during encapsulating medication in comparison with plasmid DNA, as negative charge of plasmid increases its encapsulation efficiency in PEI 25K [11,61]. However, PEI is non-degradable and molecular weight affects cytotoxicity and gene transfer activity; as high molecular weight PEI (PEI 25K) showed high transfection efficiency with significant cytotoxicity [62].…”

Section: Pdna-np Tdl (%)mentioning

confidence: 99%

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Folate Functionalized PLGA Nanoparticles Loaded with Plasmid pVAX1-NH36: Mathematical Analysis of Release

et al. 2016

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Plasmid DNA (pVAX1-NH36) was encapsulated in nanoparticles of poly-dl-lactic-coglycolic (PLGA) functionalized with polyethylene glycol (PEG) and folic acid (PLGA-PEG-FA) without losing integrity. PLGA-PEG-FA nanoparticles loaded with pVAX1-NH36 (pDNA-NPs) were prepared by using a double emulsification-solvent evaporation technique. PLGA-PEG-FA synthesis was verified by FT-IR and spectrophotometry methods. pVAX1-NH36 was replicated in Escherichia coli (E. coli) cell cultures. Atomic force microscopy (AFM) analysis confirmed pDNA-NPs size with an average diameter of 177-229 nm, depending on pVAX1-NH36 loading and zeta potentials were below −24 mV for all preparations. In vitro release studies confirmed a multiphase release profile for the duration of more than 30-days. Plasmid release kinetics were analyzed with a release model that considered simultaneous contributions of initial burst and degradation-relaxation of nanoparticles. Fitting of release model against experimental data presented excellent correlation. This mathematical analysis presents a novel approach to describe and predict the release of plasmid DNA from biodegradable nanoparticles.

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Section: Pdna-np Tdl (%)supporting

confidence: 82%

Section: Pdna-np Tdl (%)mentioning

confidence: 99%

Section: Pdna-np Tdl (%)mentioning

confidence: 99%

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Folate Functionalized PLGA Nanoparticles Loaded with Plasmid pVAX1-NH36: Mathematical Analysis of Release

et al. 2016

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“…For instance, EE% and DL% of p53 plasmid loaded PLGA microparticles were only 40.6% and 0.04%, respectively. 31 In another report, DL% of pVITRO2 loaded PLGA nanoparticles was only 0.8%. 32 Therefore, to increase the capacity …”

Section: Discussionmentioning

confidence: 99%

Pigment epithelial-derived factor gene loaded novel COOH-PEG-PLGA-COOH nanoparticles promoted tumor suppression by systemic administration

Takata

et al. 2016

IJN

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Anti-angiogenesis has been proposed as an effective therapeutic strategy for cancer treatment. Pigment epithelium-derived factor (PEDF) is one of the most powerful endogenous anti-angiogenic reagents discovered to date and PEDF gene therapy has been recognized as a promising treatment option for various tumors. There is an urgent need to develop a safe and valid vector for its systemic delivery. Herein, a novel gene delivery system based on the newly synthesized copolymer COOH-PEG-PLGA-COOH (CPPC) was developed in this study, which was probably capable of overcoming the disadvantages of viral vectors and cationic lipids/polymers-based nonviral carriers. PEDF gene loaded CPPC nanoparticles (D-NPs) were fabricated by a modified double-emulsion water-in-oil-in-water (W/O/W) solvent evaporation method. D-NPs with uniform spherical shape had relatively high drug loading (~1.6%), probably because the introduced carboxyl group in poly (D,L-lactide-co-glycolide) terminal enhanced the interaction of copolymer with the PEDF gene complexes. An excellent in vitro antitumor effect was found in both C26 and A549 cells treated by D-NPs, in which PEDF levels were dramatically elevated due to the successful transfection of PEDF gene. D-NPs also showed a strong inhibitory effect on proliferation of human umbilical vein endothelial cells in vitro and inhibited the tumor-induced angiogenesis in vivo by an alginate-encapsulated tumor cell assay. Further in vivo antitumor investigation, carried out in a C26 subcutaneous tumor model by intravenous injection, demonstrated that D-NPs could achieve a significant antitumor activity with sharply reduced microvessel density and significantly promoted tumor cell apoptosis. Additionally, the in vitro hemolysis analysis and in vivo serological and biochemical analysis revealed that D-NPs had no obvious toxicity. All the data indicated that the novel CPPC nanoparticles were ideal vectors for the systemic delivery of PEDF gene and might be widely used as systemic gene vectors.

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“…[16][17][18] Biodegradable polymers, such as poly(lactic-co-glycolic acid) (PLGA), have been widely used in the construction of porous microparticles for delivering many types of drugs to lungs, such as small molecules, proteins, plasmid DNA, and oligonucleotides. [19][20][21][22][23][24][25][26] Herein, disulfiram-loaded porous PLGA microparticle was prepared through the emulsion solvent evaporation method, in which ammonium bicarbonate was used as a porogen. The antiproliferation and antimigration efficacy of porous PLGA microparticle against NSCLC cell line, A549 and HCC827, was then systematically evaluated to get a deeper insight into the mechanism.…”

Section: Introductionmentioning

confidence: 99%

Disulfiram-loaded porous PLGA microparticle for inhibiting the proliferation and migration of non-small-cell lung cancer

Wang¹,

Yang²,

Han³

et al. 2017

IJN

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In this study, poly(lactic- co -glycolic acid) (PLGA) was used as a carrier to construct disulfiram-loaded porous microparticle through the emulsion solvent evaporation method, using ammonium bicarbonate as a porogen. The microparticle possessed highly porous surface, suitable aerodynamic diameter for inhalation (8.31±1.33 µm), favorable drug loading (4.09%±0.11%), and sustained release profile. The antiproliferation effect of release supernatant was detected through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay using non-small-cell lung cancer A549 as a model, with only 13.3% of cell viability observed for the release supernatant at 7 days. The antiproliferation mechanism was elucidated to be associated with the enhanced induction of cell apoptosis and cell cycle arrest at S phase through flow cytometry and Western blotting analysis. Finally, wound healing and transwell migration assay showed that they could efficiently inhibit the cell migration. These results demonstrated that disulfiram-loaded porous PLGA microparticle could achieve favorable antitumor efficiency, implying the potential of treating non-small-cell lung cancer in a pulmonary administration.

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Combination of doxorubicin-based chemotherapy and polyethylenimine/p53 gene therapy for the treatment of lung cancer using porous PLGA microparticles

Cited by 46 publications

References 28 publications

Folate Functionalized PLGA Nanoparticles Loaded with Plasmid pVAX1-NH36: Mathematical Analysis of Release

Folate Functionalized PLGA Nanoparticles Loaded with Plasmid pVAX1-NH36: Mathematical Analysis of Release

Pigment epithelial-derived factor gene loaded novel COOH-PEG-PLGA-COOH nanoparticles promoted tumor suppression by systemic administration

Disulfiram-loaded porous PLGA microparticle for inhibiting the proliferation and migration of non-small-cell lung cancer

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