Combination of drug-conjugated SWCNT nanocarriers for efficient therapy of cancer stem cells in a breast cancer animal model

Faraj, Achraf Al; Shaik, Asma Sultana; Ratemi, Elaref; Halwani, Rabih

doi:10.1016/j.jconrel.2016.01.053

Cited by 65 publications

(35 citation statements)

References 34 publications

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“…Salinomycin is an antibiotic and has can kill many gram positive bacteria, thus an additional potential benefit could be its anti-microbial action if used in vivo (25). Furthermore, salinomycin is also being tested as a chemotherapy for breast cancer through injection and has so far proved promising (42-44). Future studies aimed at elucidating the best delivery methods for blocking capsular contracture are needed.…”

Section: Discussionmentioning

confidence: 99%

Thy1 (CD90) Expression Is Elevated in Radiation-Induced Periprosthetic Capsular Contracture: Implication for Novel Therapeutics

Hansen

Woeller

Lacy

et al. 2017

Plastic &Amp; Reconstructive Surgery

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Background Capsular contracture is a devastating complication of post-mastectomy implant-based breast reconstruction. Unfortunately, capsular contracture rates are drastically increased by targeted radiotherapy, a standard post-mastectomy treatment. Thy1 (also called CD90) is important in myofibroblast differentiation and scar tissue formation. However, the impact of radiotherapy on Thy1 expression and the role of Thy1 in capsular contracture are unknown. Methods We analyzed Thy1 expression in primary human capsular tissue and primary fibroblast explants by RT-qPCR, Western blotting, and immunohistochemistry. Thy1 was depleted using RNA interference to determine if Thy1 expression was essential for the myofibroblast phenotype in capsular fibroblasts. Furthermore, human capsular fibroblasts were treated with a new anti-scarring compound, salinomycin to determine if Thy1 expression and myofibroblast formation were blocked by salinomycin. Results Herein, we show that radiation therapy significantly increased Thy1 mRNA and protein expression in peri-implant scar tissue. Capsular fibroblasts explanted from scar tissue retained the ability to make the myofibroblast produced scar forming components collagen I and α-smooth muscle actin (αSMA). Depletion of Thy1 decreased the fibrotic morphology of capsular fibroblasts and significantly decreased αSMA and collagen levels. Furthermore, we show for the first time that salinomycin decreased Thy1 expression and prevented myofibroblast formation in capsular fibroblasts. Conclusions These data reveal that ionizing radiation-induced Thy1 over-expression may contribute to increased capsular contracture severity, and fibroblast scar production can be ameliorated through targeting Thy1 expression. Importantly, our new results show promise for the anti-scarring ability of salinomycin in radiation-induced capsular contracture.

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Section: Discussionmentioning

confidence: 99%

Thy1 (CD90) Expression Is Elevated in Radiation-Induced Periprosthetic Capsular Contracture: Implication for Novel Therapeutics

Hansen

Woeller

Lacy

et al. 2017

Plastic &Amp; Reconstructive Surgery

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“…The current mechanistic studies suggest that GO could directly be used as a therapeutic for targeting cancer stem cells (CSCs), because of its ability to induce differentiation. In this context, we might envision that GO could be used to clear residual CSCs, with the aim of preventing tumor recurrence and distant metastasis, thereby providing a practical means for achieving “differentiation-based nano-therapy” (Fiorillo et al, 2015); (B) Schematic illustration of the preparation process of SAL-SWNTs-CHI-HA [chitosan(CHI) coated single wall carbon nanotubes (SWNTs) loaded with salinomycin (SAL) functionalized with hyaluronic acid (HA); Yao et al, 2014]; (C) Schematic diagram illustrating the concept of functionalized SWCNTs as drug carriers (Al Faraj et al, 2016b); (D) Left: schematic model showing surface and chemical structure of (ND) and Epirubicin (Epi), synthesis and aggregation of Epirubicin-nanodiamond complex (EPND). ND represented in truncated octahedron structure with different surface charge denoted with color.…”

Section: Graphene Oxidementioning

confidence: 99%

“…Soon afterwards, the same group combined Paclitaxel and Salinomycin drugs conjugated SWCNTs (Figure 2C) to actively target both breast cancer and CSCs in xenograft murine model and the results confirmed the enhanced therapeutic effect of the combined therapy compared to treatment with individual drug-conjugated nanocarriers or free drug suspensions. Thus, the developed conjugated SWCNTs drug delivery system holds great promise for effective breast cancer therapy by targeting both cancer cells and CSCs (Al Faraj et al, 2016b). …”

Section: Carbon Nanotubesmentioning

confidence: 99%

Nanomaterials in Targeting Cancer Stem Cells for Cancer Therapy

et al. 2017

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Cancer stem cells (CSCs) have been identified in almost all cancers and give rise to metastases and can also act as a reservoir of cancer cells that may cause a relapse after surgery, radiation, or chemotherapy. Thus they are obvious targets in therapeutic approaches and also a great challenge in cancer treatment. The threat presented by CSCs lies in their unlimited proliferative ability and multidrug resistance. These findings have necessitated an effective novel strategy to target CSCs for cancer treatment. Nanomaterials are on the route to providing novel methods in cancer therapies. Although, there have been a large number of excellent work in the field of targeted cancer therapy, it remains an open question how nanomaterials can meet future demands for targeting and eradicating of CSCs. In this review, we summarized recent and highlighted future prospects for targeting CSCs for cancer therapies by using a variety of nanomaterials.

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“…The antitumor effect of the coadministered group was significantly better than that of the control group and the single-loaded gene group and drug group, which indicated that co-delivery of VEGF-siRNA and DOX had a synergistic effect. 53 The expression of VEGF protein in vivo is shown in Figure 15. ELISA was used to evaluate the relation between the tumor inhibitory activity and the suppressed VEGF expression.…”

Section: Gene Silencing Efficiency Of Gpf/dox/ Vegf-sirna On Protein mentioning

confidence: 99%

Doxorubicin and anti-VEGF siRNA co-delivery via nano-graphene oxide for enhanced cancer therapy in vitro and in vivo

Sun¹,

Wang²,

Cui³

et al. 2018

IJN

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BackgroundGraphene oxide (GO) has attracted intensive interest in biological and medical fields in recent years due to its unique physical, chemical, and biological properties. In our previous work, we proved that GO could deliver small interfering RNA (siRNA) into cells and downregulate the expression of the desired gene.MethodsThis study investigated the potential of a modified GO nanocarrier for co-delivery of siRNA and doxorubicin (DOX) for enhanced cancer therapy. Fourier transform infrared spectroscopy, laser particle size analyzer, UV-visible spectroscopy, gel electrophoresis retardation, and in vitro release assay were studied.ResultsThe results of real-time polymerase chain reaction revealed that the expression of vascular endothelial growth factor (VEGF) mRNA was decreased 46.84%±3.72% (mean ± SD). Enzyme-linked immunosorbent assay indicated that the expression of VEGF protein was down-regulated to 52.86%±1.10% (mean ± SD) in vitro. In vivo tumor growth assay GO-poly-l-lysine hydrobromide/folic acid (GPF)/DOX/siRNA exhibited gene silencing and tumor inhibition (66.95%±2.35%, mean ± SD) compared with naked siRNA (1.62%±1.47%, mean ± SD) and DOX (33.63%±5.85%, mean ± SD). GPF/DOX/siRNA exhibited no testable cytotoxicity.ConclusionThe results indicated that co-delivery of siRNA and DOX by GPF could be a promising application in tumor clinical therapy.

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Combination of drug-conjugated SWCNT nanocarriers for efficient therapy of cancer stem cells in a breast cancer animal model

Cited by 65 publications

References 34 publications

Thy1 (CD90) Expression Is Elevated in Radiation-Induced Periprosthetic Capsular Contracture: Implication for Novel Therapeutics

Thy1 (CD90) Expression Is Elevated in Radiation-Induced Periprosthetic Capsular Contracture: Implication for Novel Therapeutics

Nanomaterials in Targeting Cancer Stem Cells for Cancer Therapy

Doxorubicin and anti-VEGF siRNA co-delivery via nano-graphene oxide for enhanced cancer therapy in vitro and in vivo

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