Combination of edaravone and neural stem cell transplantation repairs injured spinal cord in rats

Song, Yueming; Peng, Chuangang; Ye, X B

doi:10.4238/2015.december.29.23

Cited by 11 publications

(6 citation statements)

References 15 publications

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“…The second evaluation was the MRI, which showed in the EB-treated group, a diffuse hyperintense sclerotic plaque in the T2-weighted and axial scan and hypointense sclerotic plaque in the T1-weighted scan. The SVF co-treated group had a decrease in lesion intensity on the T1-weighted, T2-weighted, and axial scans as mentioned by [ 66 ] in the dog with spinal cord injury and [ 67 ]. This is unlike the study mentioned by [ 62 ], which found no improvement in MRI lesions after stem cell injection.…”

Section: Discussionmentioning

confidence: 78%

Laser-activated autologous adipose tissue-derived stromal vascular fraction restores spinal cord architecture and function in multiple sclerosis cat model

et al. 2023

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Background Multiple sclerosis (MS) is the most frequent non-traumatic neurological debilitating disease among young adults with no cure. Over recent decades, efforts to treat neurodegenerative diseases have shifted to regenerative cell therapy. Adipose tissue-derived stromal vascular fraction (SVF) comprises a heterogeneous cell population, considered an easily accessible source of MSCs with therapeutic potential in autoimmune diseases. This study aimed to assess the regenerative capacity of low-level laser-activated SVF in an MS cat model. Methods Fifteen adult Persian cats were used in this study: Group I (control negative group, normal cats), Group II (EB-treated group, induced for MS by ethidium bromide (EB) intrathecal injection), and Group III (SVF co-treated group, induced for MS then treated with SVF on day 14 post-induction). The SVF was obtained after digesting the adipose tissue with collagenase type I and injecting it intrathecal through the foramen magnum. Results The results showed that the pelvic limb’s weight-bearing locomotion activity was significantly (P ≤ 0.05) recovered in Group III, and the Basso, Beattie, and Bresnahan (BBB) scores of hindlimb locomotion were significantly higher in Group III (14 ± 0.44) than Group II (4 ± 0.31). The lesion’s extent and intensity were reduced in the magnetic resonance imaging (MRI) of Group III. Besides, the same group showed a significant increase in the expression of neurotrophic factors: BDNF, SDF and NGF (0.61 ± 0.01, 0.51 ± 0.01 and 0.67 ± 0.01, respectively) compared with Group II (0.33 ± 0.01, 0.36 ± 0.006 and 0.2 ± 0.01, respectively). Furthermore, SVF co-treated group revealed a significant (P ≤ 0.05) increase in oligodendrocyte transcription factor (Olig2) and myelin basic protein (4 ± 0.35 and 6 ± 0.45, respectively) that was decreased in group II (1.8 ± 0.22 and 2.9 ± 0.20, respectively). Moreover, group III showed a significant (P ≤ 0.05) reduction in Bax and glial fibrillary acidic protein (4 ± 0.53 and 3.8 ± 0.52, respectively) as compared with group II (10.7 ± 0.49 and 8.7 ± 0.78, respectively). The transmission electron microscopy demonstrated regular more compact, and markedly (P ≤ 0.05) thicker myelin sheaths (mm) in Group III (0.3 ± 0.006) as compared with group II (0.1 ± 0.004). Based on our results, the SVF co-treated group revealed remyelination and regeneration capacity with a reduction in apoptosis and axonal degeneration. Conclusion SVF is considered an easy, valuable, and promising therapeutic approach for treating spinal cord injuries, particularly MS.

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Section: Discussionmentioning

confidence: 78%

Laser-activated autologous adipose tissue-derived stromal vascular fraction restores spinal cord architecture and function in multiple sclerosis cat model

et al. 2023

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“…For instance, NSC transplantation together with erythropoietin injection has been reported to positively affect axon regeneration in adult rats having suffered transected spinal cord injuries (Zhao et al, 2015). In addition, a combination of edaravone and NSC transplantation has been shown to repair injured spinal cords in a rat model (Song et al, 2015). Moreover, GM1 and NGF synergistically effect neurite regeneration, neurofilament protein upregulation, and stimulation of neuritogenesis (Bolsover, 2005).…”

Section: Discussionmentioning

confidence: 99%

Effects of ganglioside GM1 and neural growth factor on neural stem cell proliferation and differentiation

Wang

Song

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Neurogenesis, recovery from nerve injury, neurodegeneration, and Parkinson's disease affect people's health, yet the underlying molecular mechanisms remain elusive. Here, we investigated the effect of ganglioside GM1 and neural growth factor (NGF) on neural stem cell (NSC) proliferation and differentiation in vitro to provide a scientific basis for comprehensive treatment of nervous system diseases via NSC application. As widely applied methods of relatively high accuracy, cell counts and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assays were used to assess NSC proliferation. In addition, western blotting 2 Q. Wang et al. Genetics and Molecular Research 15 (3): gmr.15038376 was employed to determine NSC differentiation. Cell counts and MTT assays demonstrated that in epidermal growth factor (EGF)-and basic fibroblast growth factor (bFGF)-containing medium, a high concentration of GM1, but not NGF, significantly elevated NSC proliferation. In NSC cultures lacking EGF and bFGF, cell counts and MTT values were significantly increased compared to those in the negative control group on days 4, 7, and 10 after GM1 (25, 100, and 200 ng/mL) but not NGF (25, 50, 100, and 200 ng/mL) treatment. Western blotting revealed significantly increased expression of nestin (an NSC marker) in NSCs treated with GM1, and upregulation of glial fibrillary acidic protein (a glial cell marker) and neuron-specific enolase (a neuron marker) in those administered NGF. Our results suggest that GM1 and NGF induce NSC proliferation and differentiation, respectively, in a dose-dependent manner.

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“…Transplanting neural stem cells construct significant improvement in spinal cord-injured animal models, especially, at the acute phase of injury by means of axonal growth promotion as well as lost cell replacement (Cheng et al, 2017;Iwanami et al, 2005;Lu, Jones, Snyder, & Tuszynski, 2003). Thus, the low efficiency of NSCs transplantation is a challenging problem (Lin et al, 2016), whereas, combinational therapy, the chemical application along with NSCs, may provide a protective circumstance for transplanted NSCs survival and operation (Hosseini et al, 2018;Lane, Lepore, & Fischer, 2017;Song, Peng, & Ye, 2015;Zhang & Shen, 2015).…”

Section: Introductionmentioning

confidence: 99%

Combinational therapy of lithium and human neural stem cells in rat spinal cord contusion model

Mohammadshirazi

Sadrosadat

Jaberi

et al. 2019

Journal Cellular Physiology

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A large number of treatment approaches have been used for spinal cord injury improvement, a medically incurable disorder, and subsequently stem cell transplantation appears to be a promising strategy. The main objective of this study is to ascertain whether combinational therapy of human neural stem cells (hNSCs) together with lithium chloride improves cell survival, proliferation, and differentiation in a rat spinal contusion model, or not.Contusive spinal cord injury was implemented on Wistar male rats. Experimental groups comprised of: control, hNSCs transplanted, lithium chloride (Li), and hNSCs and lithium chloride (hNSCs + Li). In every experimental group, locomotor activity score and motor evoked potential (MEP) were performed to evaluate motor recovery as well as histological assessments to determine mechanisms of improvement.In accordance with our results, the hNSCs + Li and the Li groups showed significant improvement in locomotor scores and MEP. Also, Histological assessments revealed that transplanted hNSCs are capable of differentiation and migration along the spinal cord. Although NESTIN-positive cells were proliferated significantly in the Lithium group in comparison with control and the hNSCs + Li groups, the quantity of ED1 cells in the hNSCs + Li was significantly larger than the other two groups.Our results demonstrate that combinational therapy of hNSCs with lithium chloride and lithium chloride individually are adequate for ameliorating more than partial functional recovery and endogenous repair in spinal cord-injured rats.

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Combination of edaravone and neural stem cell transplantation repairs injured spinal cord in rats

Cited by 11 publications

References 15 publications

Laser-activated autologous adipose tissue-derived stromal vascular fraction restores spinal cord architecture and function in multiple sclerosis cat model

Laser-activated autologous adipose tissue-derived stromal vascular fraction restores spinal cord architecture and function in multiple sclerosis cat model

Effects of ganglioside GM1 and neural growth factor on neural stem cell proliferation and differentiation

Combinational therapy of lithium and human neural stem cells in rat spinal cord contusion model

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