Combination of Ethyl Acetate Extract of Trichoderma hamatum Fermentation Broth and Fungicide Carbendazim enhances Inhibition against Scleromitrula shiraiana under Laboratory Conditions

Chen, Xiaqing; Huang, Yü; Huang, Zhen

doi:10.17582/journal.pjz/2018.50.6.2239.2247

Cited by 4 publications

(3 citation statements)

References 23 publications

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“…Bioactive secondary metabolites of T. asperellum were extracted using ethyl acetate solvent, as previously described by Chen et al (2018) . Trichoderma was grown in a PDB medium and incubated on a rotary shaker (150 rpm) for 21 days at 28°C.…”

Section: Methodsmentioning

confidence: 99%

Trichoderma asperellum empowers tomato plants and suppresses Fusarium oxysporum through priming responses

et al. 2023

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Plant-associated microbes play crucial roles in plant health and promote growth under stress. Tomato (Solanum lycopersicum) is one of the strategic crops grown throughout Egypt and is a widely grown vegetable worldwide. However, plant disease severely affects tomato production. The post-harvest disease (Fusarium wilt disease) affects food security globally, especially in the tomato fields. Thus, an alternative effective and economical biological treatment to the disease was recently established using Trichoderma asperellum. However, the role of rhizosphere microbiota in the resistance of tomato plants against soil-borne Fusarium wilt disease (FWD) remains unclear. In the current study, a dual culture assay of T. asperellum against various phytopathogens (e.g., Fusarium oxysporum, F. solani, Alternaria alternata, Rhizoctonia solani, and F. graminerarum) was performed in vitro. Interestingly, T. asperellum exhibited the highest mycelial inhibition rate (53.24%) against F. oxysporum. In addition, 30% free cell filtrate of T. asperellum inhibited F. oxysporum by 59.39%. Various underlying mechanisms were studied to explore the antifungal activity against F. oxysporum, such as chitinase activity, analysis of bioactive compounds by gas chromatography–mass spectrometry (GC–MS), and assessment of fungal secondary metabolites against F. oxysporum mycotoxins in tomato fruits. Additionally, the plant growth-promoting traits of T. asperellum were studied (e.g., IAA production, Phosphate solubilization), and the impact on tomato seeds germination. Scanning electron microscopy, plant root sections, and confocal microscopy were used to show the mobility of the fungal endophyte activity to promote tomato root growth compared with untreated tomato root. T. asperellum enhanced the growth of tomato seeds and controlled the wilt disease caused by the phytopathogen F. oxysporum by enhancing the number of leaves as well as shoot and root length (cm) and fresh and dry weights (g). Furthermore, Trichoderma extract protects tomato fruits from post-harvest infection by F. oxysporum. Taking together, T. asperellum represents a safe and effective controlling agent against Fusarium infection of tomato plants.

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Section: Methodsmentioning

confidence: 99%

Trichoderma asperellum empowers tomato plants and suppresses Fusarium oxysporum through priming responses

et al. 2023

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“…The bioactive metabolites of B. bassiana applied with Si NPs were extracted from CFS by liquid-liquid extraction as described by Chen et al [ 88 ] using ethyl acetate 3 times via a separating funnel in a ratio of (0.5:1 (v∕v) solvent: filtrate) and sonicated for 15 min. The separated organic layer was collected and filtered over anhydrous sodium sulphate (Na 2 SO 4 ).…”

Section: Methodsmentioning

confidence: 99%

Prospective of mycorrhiza and Beauvaria bassiana silica nanoparticles on Gossypium hirsutum L. plants as biocontrol agent against cotton leafworm, Spodoptera littoralis

et al. 2022

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Background Plant-herbivorous insects are a severe danger to the world’s agricultural production of various crops. Insecticides used indiscriminately resulted in habitat destruction due to their high toxicity, as well as disease resistance. In this respect, the development of a sustainable approach to supreme crop production with the least damage is a crucially prerequisite. As a result, the current study was carried out to understand the potential effect of arbuscular mycorrhizal (AM) fungi along with Beauvaria bassiana silica nanoparticles (Si NPs) as a new approach to increase cotton (Gossypium hirsutum L. Merr.) defense against an insect herbivore, Spodoptera littoralis. AM and non-AM cotton plants were infested with S. littoralis and then sprayed with a biopesticide [B. bassiana Si NPs] or a chemical insecticide (Chlorpyrifos). Results The gas chromatography-mass spectrometry (GC–MS) analysis of B. bassiana Si NPs fungal extract showed that the major constituents identified were Oleyl alcohol, trifluoroacetate, 11-Dodecen-1-AL and 13-Octadecenal, (Z)-(CAS). Besides, results revealed a highly significant decrease in growth parameters in S. littoralis infested plants, however, with AM fungal inoculation a substantial improvement in growth traits and biochemical parameters such as protein and carbohydrates contents was observed. In addition, stimulation in proline and antioxidant enzymes activity and a decrease in malondialdehyde content were observed after AM inoculation. Conclusion AM fungi mitigate the harmful effects of herbivorous insects by strengthening the cotton plant’s health via enhancing both morphological and biochemical traits that can partially or completely replace the application of chemical insecticides.

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“…The seed inoculum was added to fresh broth medium (4 Littre total) and the mixture was incubated at 25 ± 1°C for 21 days. After incubation period, the metabolites were extracted as described method by Chen et al (2018) using ethyl acetate (EthOAc) from the cell-free culture supernatant. Aliquots of the fungal culture were mixed with a half volume of ethyl acetate (1 : 0.5) and mixed vigorously then put the mixtures in separating funnel and leave them 2 hr.…”

Section: Fungal Isolatesmentioning

confidence: 99%

Insecticidal Activity of Secondary Metabolites of Locally Isolated Fungal Strains against some Cotton Insect Pests

Abdullah

2019

Journal of Plant Protection and Pathology

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Microbial pest control is one of the best alternatives to chemical insecticides. Beauveria bassiana and Trichoderma harzianum are important entomopathogens of many insect pests. Cotton leafworm (Spodoptera littoralis) and aphids (Aphis gossypii) are the most destructive pests in crop production such as cotton, cabbage, tomato, potato, and lettuce. The present study sought to isolate both fungal strains (B. bassiana and T. harzianum) and extract their secondary metabolites by ethyl acetate then evaluate the efficacy of crude extract against both insect pests (S. littoralis and A. gossypii). Also, Gas chromatographymass spectrometry (GC-MS) analysis was conducted to both extracts of isolated fungi. The results indicated that both extracts of tested fungi have insecticidal activity against both tested insects. B. bassiana extract was more toxic against both insects than T. harzianum. The extract of T. harzianum was more toxic against A. gossypii more than S. littoralis. The LC 50 values of B. bassiana and T. harzianum against S. littoralis were 575 and 3238 ppm respectively but, they were 226 and 389 ppm against A. gossypii respectively. GC-MS analysis indicated the presence of some identified compounds as insecticides in both extracts of fungi such as: n-Hexadecanoic acid; Hexadecanoic acid, methyl ester; 9,12-Octadecadienoic acid, methyl ester, (E,E)-; Tetradecanoic acid, 12-methyl-, methyl ester; 7,10-Octadecadienoic acid, methyl ester and trans-13-Octadecenoic acid. This study recommended that the ethyl acetate extract of both tested fungi can be used as an alternative of chemical pesticides to control A. gossypii but in the case of S. littoralis can be used the extract of B. bassiana only.

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Combination of Ethyl Acetate Extract of Trichoderma hamatum Fermentation Broth and Fungicide Carbendazim enhances Inhibition against Scleromitrula shiraiana under Laboratory Conditions

Cited by 4 publications

References 23 publications

Trichoderma asperellum empowers tomato plants and suppresses Fusarium oxysporum through priming responses

Trichoderma asperellum empowers tomato plants and suppresses Fusarium oxysporum through priming responses

Prospective of mycorrhiza and Beauvaria bassiana silica nanoparticles on Gossypium hirsutum L. plants as biocontrol agent against cotton leafworm, Spodoptera littoralis

Insecticidal Activity of Secondary Metabolites of Locally Isolated Fungal Strains against some Cotton Insect Pests

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