Combination of LC–MS based metabolomics and antioxidant activity for evaluation of bioactive compounds in Fragaria vesca leaves from Italy

D’Urso, Gilda; Pìzza, Cosimo; Piacente, Sonia; Montoro, Paola

doi:10.1016/j.jpba.2017.12.005

Cited by 37 publications

(39 citation statements)

References 24 publications

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“…Caesalpinia decapetala (C. decapetala) is effective in the oxidation stability of an oil-in-water emulsion (Gallego et al 2017). Analysis using LC-ESI/LTQ Orbitrap/MS of autochthonous germplasm of the Campania region showed a higher level of anti-oxidant activity compared with the non-autochthonous germplasm (D'Urso et al 2018). Enzymatic glucosylation of caffeic acid and EGCG leads to improved anti-oxidant ability in a cellular model of UV-induced skin ageing (Nadim et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Activity of catechins and their applications

et al. 2020

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Background: Catechins, which are polyphenol compounds found in many plants and are an important component of tea leaves, are strong anti-oxidants. Research: Many studies seek to enhance the effects of catechins on the human body and boost their protective power against UV radiation. There are many examples of the positive anti-microbial, anti-viral, anti-inflammatory, anti-allergenic, and anti-cancer effects of catechins. Catechins increase the penetration and absorption of healthy functional foods and bio cosmetics into the body and the skin, thus improving their utility. High value-added antioxidant substances have been extracted from food and plant sludge, and experiments have shown that catechins are safe when applied to the human body. The stability of catechins is very important for their absorption into the human body and the effectiveness of their anti-oxidant properties.Conclusion: Continued research on the strong anti-oxidant effects of catechins is expected to result in many advances in the food, cosmetics, and pharmaceutical industries.

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Section: Introductionmentioning

confidence: 99%

Activity of catechins and their applications

et al. 2020

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“…The great advancements of sequencing techniques, high-throughput analytical platforms and chemometric Biomolecules 2020, 10, 287 2 of 17 tools that have taken place over the past few decades stood behind the emergence and prosperity of the omics technologies [3]. Recently, metabolomics has become a routine approach in studies aiming to evaluate the activities of medicinal plants or to characterize the metabolites responsible for such activities [4][5][6][7]. Classical methods for determination of the active plant metabolites rely on the activity-guided fractionation and purification techniques.…”

Section: Introductionmentioning

confidence: 99%

Investigation of α-Glucosidase Inhibitory Metabolites from Tetracera scandens Leaves by GC–MS Metabolite Profiling and Docking Studies

et al. 2020

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Stone leaf (Tetracera scandens) is a Southeast Asian medicinal plant that has been traditionally used for the management of diabetes mellitus. The underlying mechanisms of the antidiabetic activity have not been fully explored yet. Hence, this study aimed to evaluate the α-glucosidase inhibitory potential of the hydromethanolic extracts of T. scandens leaves and to characterize the metabolites responsible for such activity through gas chromatography–mass spectrometry (GC–MS) metabolomics. Crude hydromethanolic extracts of different strengths were prepared and in vitro assayed for α-glucosidase inhibition. GC–MS analysis was further carried out and the mass spectral data were correlated to the corresponding α-glucosidase inhibitory IC50 values via an orthogonal partial least squares (OPLS) model. The 100%, 80%, 60% and 40% methanol extracts displayed potent α-glucosidase inhibitory potentials. Moreover, the established model identified 16 metabolites to be responsible for the α-glucosidase inhibitory activity of T. scandens. The putative α-glucosidase inhibitory metabolites showed moderate to high affinities (binding energies of −5.9 to −9.8 kcal/mol) upon docking into the active site of Saccharomyces cerevisiae isomaltase. To sum up, an OPLS model was developed as a rapid method to characterize the α-glucosidase inhibitory metabolites existing in the hydromethanolic extracts of T. scandens leaves based on GC–MS metabolite profiling.

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“…Decoctions of leaves are used for hypertension treatment and show diuretic and dermatological protective properties. Metabolite profiling analysis in strawberry leaves in combination with antioxidant measurement was performed to identify natural products that are accountable for the antioxidant activity of the extracts (D’Urso et al 2018). LC–MS was also utilized to study the differences and commonalities between the metabolite profiles in leaves (Kårlund et al 2017).…”

Section: Biological Questionsmentioning

confidence: 99%

Answering biological questions by analysis of the strawberry metabolome

et al. 2018

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Background The qualitative and quantitative analysis of all low molecular weight metabolites within a biological sample, known as the metabolome, provides powerful insights into their roles in biological systems and processes. The study of all the chemical structures, concentrations, and interactions of the thousands of metabolites is called metabolomics. However present state of the art methods and equipment can only analyse a small portion of the numerous, structurally diverse groups of chemical substances found in biological samples, especially with respect to samples of plant origin with their huge diversity of secondary metabolites. Nevertheless, metabolite profiling and fingerprinting techniques have been applied to the analysis of the strawberry metabolome since their early beginnings. Aim The application of metabolomics and metabolite profiling approaches within strawberry research was last reviewed in 2011. Here, we aim to summarize the latest results from research of the strawberry metabolome since its last review with a special emphasis on studies that address specific biological questions. Key scientific concepts Analysis of strawberry, and other fruits, requires a plethora of analytical methods and approaches encompassing the analysis of primary and secondary metabolites, as well as capturing and quantifying volatile compounds that are related to aroma as well as fruit development, function and plant-to-plant communication. The success and longevity of metabolite and volatile profiling approaches in fruit breeding relies upon the ability of the approach to uncover biologically meaningful insights. The key concepts that must be addressed and are reviewed include: gene function analysis and genotype comparison, analysis of environmental effects and plant protection, screening for bioactive compounds for food and non-food uses, fruit development and physiology as well as fruit sensorial quality. In future, the results will facilitate fruit breeding due to the identification of metabolic QTLs and candidate genes for fruit quality and consumer preference.

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Combination of LC–MS based metabolomics and antioxidant activity for evaluation of bioactive compounds in Fragaria vesca leaves from Italy

Cited by 37 publications

References 24 publications

Activity of catechins and their applications

Activity of catechins and their applications

Investigation of α-Glucosidase Inhibitory Metabolites from Tetracera scandens Leaves by GC–MS Metabolite Profiling and Docking Studies

Answering biological questions by analysis of the strawberry metabolome

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