Combination of medicinal honey and 904 nm superpulsed laser-mediated photobiomodulation promotes healing and impedes inflammation, pain in full-thickness burn

Yadav, Anju; Verma, Saurabh; Keshri, Gaurav K.; Gupta, Asheesh

doi:10.1016/j.jphotobiol.2018.07.008

Cited by 42 publications

(41 citation statements)

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“…It has previously been reported that 632.8 nm laser irradiation (4.5 mW) promoted skeletal muscle regeneration via activation of MAPK/ERK cascades . The present findings are in agreement with the previous study, where superpulsed 904 nm PBM resulted in increased expression of Na + /K + ‐ATPase during burn wound healing in rats .…”

Section: Discussionsupporting

confidence: 93%

“…The present study exhibited that pulsed 810 nm PBM attenuates inflammation and induces antinociception by lowering the levels of inflammatory and pain markers. These findings well‐corroborated with our previous studies, in which PBM with pulsed 810 nm and superpulsed 904 nm lasers resulted in lowered levels of TNF‐α, nuclear factor‐κB, IL‐1β, COX‐2, and SPR, respectively, which attenuated the exaggerated inflammatory response during chronic wound healing .…”

Section: Discussionsupporting

confidence: 91%

“…Similarly, Na + /K + ‐ATPase also plays a key role in tissue repair process by regulating the ionic transport. Na + /K + ‐ATPase is involved in laser‐mediated respiratory chain activation and modulates DNA and RNA synthesis . In the present study, we found that pulsed 810 nm PBM triggered cell survival and proliferation cascades as evidenced by enhanced protein expression of Akt, ERK, and Na + /K + ‐ATPase to promote wound repair processes and restore normal functions of the skin.…”

Section: Discussionsupporting

confidence: 55%

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Effects of Pulsed 810 nm Al‐Ga‐As Diode Laser on Wound Healing Under Immunosuppression: A Molecular Insight

et al. 2019

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Background and Objectives Dysregulated inflammation is one of the major contributing factors for the prevalence of non‐healing chronic wound in immunosuppressed subjects. Photobiomodulation (PBM) has emerged as a potential non‐thermal, light‐based therapeutic healing intervention for the treatment of impaired wounds. Study Design/Materials and Methods The present study delineates the underlying molecular mechanisms of PBM 810 nm laser‐induced full‐thickness cutaneous wound repair in immunosuppressed rats at continuous and pulsed wave‐mode with power‐density of 40 mW/cm 2, fluence 22.6 J/cm 2 for 10 minutes daily for 7 post‐wounding days. Molecular markers were assessed using biochemical, enzyme‐linked immunosorbent assay quantification, enzyme kinetics and immunoblots analyses pertaining to inflammation, oxidative stress, cell survival, calcium signaling, and proliferation cascades. Results Results distinctly revealed that pulsed 810 nm (10 Hz) PBM potentially influenced the cell survival and proliferation signaling pathway by significantly upregulated phospho‐protein kinase B(phospho‐Akt), phospho‐extracellular‐signal‐regulated kinase 1 (ERK1), transient receptor potential vanilloid‐3 (TRPV3), Ca2+, calmodulin, transforming growth factor‐β1 (TGF‐β1), TGF‐βR3, and Na +/K +‐ATPase pump levels. PBM treatment resulted in reduction of exaggerated inflammatory responses evident by significantly repressed levels of interleukin‐1β (IL‐1β), IL‐6, cyclooxygenase 2 (COX‐2), and substance‐P receptor (SPR), as well as inhibited apoptotic cell death by decreasing p53, cytochrome C, and caspase 3 levels (P < 0.05), which, in turn, effectively augment the wound repair in immunosuppressed rats. PBM treatment also lowered 4‐hydroxynoneal (HNE) adduct level and NADP/NADPH ratio and upregulated the GRP78 expression, which might culminate into reduced oxidative stress and maintained the redox homeostasis. Conclusions Taken together, these findings would be helpful in better understanding of the molecular aspects involved in pulsed 810 nm laser‐mediated dermal wound healing in immunosuppressed rats through regulation of cell survival and proliferation via Ca2+‐calmodulin, Akt, ERK, and redox signaling. Lasers Surg. Med. © 2019 Wiley Periodicals, Inc.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 55%

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Effects of Pulsed 810 nm Al‐Ga‐As Diode Laser on Wound Healing Under Immunosuppression: A Molecular Insight

et al. 2019

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“…In this study, the radiation of low-level laser (660 nm) started 30 minutes after the induction of injury to prevent the expansion of the injury as much as possible before the beginning of destructive events. Likewise, the improvement of motion observed from the first week of the treatment in this study can be the evidence to the fact that the use of LLL, a well-known therapeutic method to control inflammatory, can be a good alternative in the first line of treatment after SCI (13,(22)(23)(24)(25). LLL reduces the secretion of cytokines and inflammatory factors, such as TNF-α, IL-1β, and IL-6 that were increased due to the inflammation (26).…”

Section: Discussionsupporting

confidence: 57%

The Effect of Four Weeks of Low-Level Laser Radiation (660 nm) on Movement Recovery and Fibroblasts Invasion

et al. 2019

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Background: Despite the fact that many researches have been done in regards to spinal cord injury (SCI), it remains an undissolved problem that has an effect on the individual's life and society. It could be due to complicated events that occur after SCI. Inflammation is an important initiator for after SCI events and low-level laser (LLL), which is known as an anti-inflammatory agent. Objectives: The aim of this study is the evaluation effects of LLL on the fibroblast invasion, axon number, and functional recovery after SCI. Methods: Twenty-four adult male Wistar rats were randomly divided into three groups as control, SCI, SCI + laser. Thirty minutes after SCI that was induced by aneurysm clip LLL irritated for 45 seconds daily and continued for four weeks. Functional recovery by BBB score, fibroblast invasion by H&E, and Axons number by Bielschowsky silver staining was assessed. Results: The data indicated LLL therapy improved BBB score, increased axons number, and decreased fibroblasts invasion around cavity comparing to SCI animals (P < 0.001). Conclusions: Our result showed that the initiation LLL therapy, in a short time after SCI, could be effective in the reduction of SCI side effects and improved functional recovery.

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“… 30 , 31 PBM-based therapies are non-invasive and stimulate cellular pathways in wound repair and regeneration. 32 , 33 In recent investigations, the combined therapy of metal-based nanoparticles with PBM has been studied in the treatment of wounds ( in vivo ). The synergistic effect of this combined therapy has shown antibacterial activity against pathogenic bacteria as well as the promotion of wound healing.…”

Section: Resultsmentioning

confidence: 99%

<p>Selective Laser Efficiency of Green-Synthesized Silver Nanoparticles by <em>Aloe arborescens</em> and Its Wound Healing Activities in Normal Wounded and Diabetic Wounded Fibroblast Cells: In vitro Studies</p>

Kumar¹,

Houreld²,

Abrahamse³

2020

IJN

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Introduction Silver nanoparticles (AgNPs) have been extensively used in wound healing applications owing to their valuable physicochemical and biological properties. The main objective of this study was to evaluate the combined effects of green-synthesized silver nanoparticles (G-AgNPs) and photobiomodulation (PBM; laser irradiation at 830 nm with 5 J/cm 2 ) in normal wounded and diabetic wounded fibroblast cells (WS1). Methods The combined effect of G-AgNPs and PBM was studied by various in vitro wound healing studies including cell morphology, cell migration rate and percentage wound closure, cell viability, cell proliferation, and filamentous (F)-actin and nuclear morphology staining. Results Cell viability results revealed good cellular compatibility of G-AgNPs to WS1 cells. The combined therapy of G-AgNPs and PBM demonstrated promising results to achieve progressive migration and wound closure in both normal wounded and diabetic wounded cell models. G-AgNPs alone and in combination with PBM had no negative effect on cell viability and proliferation, and there was an increase in cell migration. Conclusion Overall, these findings demonstrate that the combined treatment of G-AgNPs and PBM does not display any adverse effects on wound healing processes in both normal wounded and diabetic wounded cell models.

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Combination of medicinal honey and 904 nm superpulsed laser-mediated photobiomodulation promotes healing and impedes inflammation, pain in full-thickness burn

Cited by 42 publications

References 37 publications

Effects of Pulsed 810 nm Al‐Ga‐As Diode Laser on Wound Healing Under Immunosuppression: A Molecular Insight

Effects of Pulsed 810 nm Al‐Ga‐As Diode Laser on Wound Healing Under Immunosuppression: A Molecular Insight

The Effect of Four Weeks of Low-Level Laser Radiation (660 nm) on Movement Recovery and Fibroblasts Invasion

<p>Selective Laser Efficiency of Green-Synthesized Silver Nanoparticles by <em>Aloe arborescens</em> and Its Wound Healing Activities in Normal Wounded and Diabetic Wounded Fibroblast Cells: In vitro Studies</p>

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Combination of medicinal honey and 904 nm superpulsed laser-mediated photobiomodulation promotes healing and impedes inflammation, pain in full-thickness burn

Cited by 42 publications

References 37 publications

Effects of Pulsed 810 nm Al‐Ga‐As Diode Laser on Wound Healing Under Immunosuppression: A Molecular Insight

Effects of Pulsed 810 nm Al‐Ga‐As Diode Laser on Wound Healing Under Immunosuppression: A Molecular Insight

The Effect of Four Weeks of Low-Level Laser Radiation (660 nm) on Movement Recovery and Fibroblasts Invasion

<p>Selective Laser Efficiency of Green-Synthesized Silver Nanoparticles by <em>Aloe arborescens</em> and Its Wound Healing Activities in Normal Wounded and Diabetic Wounded Fibroblast Cells: In vitro Studies</p>

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Combination of medicinal honey and 904 nm superpulsed laser-mediated photobiomodulation promotes healing and impedes inflammation, pain in full-thickness burn