Combination of Panax ginseng and Diospyros kaki Leaf Inhibits White Adipocyte Differentiation and Browning Process through AMP-Activated Protein Kinase (AMPK) Activation In Vitro and In Vivo

Lee, Hwa Young; Lee, Geum-Hwa; Kim, Hwa-Jin; Lim, Young Jae; Ko, Byung Joon; Kim, Do‐Sung; Kim, Tae Won; Kim, Hye Kyung; Kim, Tae Young; Hwang, Dae Il; Choi, Ha Kyoung; Ju, Seon Min; Min, Kyung Hyun; Chae, Han-Jung

doi:10.3390/nu15122776

Cited by 3 publications

(1 citation statement)

References 32 publications

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“…Certain foods have the ability to promote the browning of white adipocytes by activating AMPK. The combination of Panax ginseng and Diospyros kaki leaf has the potential to inhibit adipogenesis and induce the browning process in adipocytes through the activation of AMPK [13]. Black wheat extract and strawberry methanolic extract have regulatory effects of browning in 3T3−L1 adipocytes through the activation of the AMPK pathway [14,15].…”

Section: Introductionmentioning

confidence: 99%

Rabbit Meat Extract Induces Browning in 3T3−L1 Adipocytes via the AMP−Activated Protein Kinase Pathway

Bae,

Lee,

Cho

et al. 2023

Foods

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The browning of white adipocytes may be an innovative approach to address obesity. This study investigated the effects of rabbit meat extract on 3T3−L1 adipocytes, with a specific emphasis on inducing browning. The browning effects of rabbit meat extract were evaluated by analyzing genes specifically expressed in 3T3−L1 adipocytes using quantitative PCR and immunoblotting. Rabbit meat extract increased the expression of brown adipocyte−specific markers, UCP1 and PGC1α, and mitochondrial biogenesis factors, TFAM and NRF1, without affecting cell viability in fully differentiated 3T3−L1 adipocytes. Moreover, adipocyte differentiation and the triglyceride content were decreased; hormone−sensitive lipase activity was promoted. Rabbit meat extract activated the AMPK pathway in the differentiated 3T3−L1 cells. However, in adipocytes treated with rabbit meat extract, the expression of genes related to browning was reduced by the AMP−activated protein kinase (AMPK) inhibitor, dorsomorphin dihydrochloride. To the best of our knowledge, this is the first study to demonstrate that rabbit meat extract induces the browning of white adipocytes via the activation of the AMPK pathway, thereby demonstrating its therapeutic potential in preventing obesity.

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Section: Introductionmentioning

confidence: 99%

Rabbit Meat Extract Induces Browning in 3T3−L1 Adipocytes via the AMP−Activated Protein Kinase Pathway

Bae,

Lee,

Cho

et al. 2023

Foods

View full text Add to dashboard Cite

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Discussion on the treatment of diabetic kidney disease based on the “gut–fat–kidney” axis

He,

Jia,

Chen

et al. 2024

Int Urol Nephrol

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Ginseng Soluble Dietary Fiber Enhances Spermatogenic Potential in Obese Mice via the MAPK Signaling Pathway

Zhang,

Yu,

Bai

et al. 2024

Journal of Food Biochemistry

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Objectives. To investigate the effects of ginseng soluble dietary fiber (GSDF) on the spermatogenic potential in high‐fat diet (HFD)‐induced obese mice. Method. C57BL/6 mice were fed an HFD for 60 days, and GSDF was administered by gastric gavage. The mice were divided into control, HFD, GSDF (high, medium, and low), and positive (metformin and MH) groups. During this period, changes in body weight were recorded. Various organ indices were measured 24 h after the last dose. Sperm quality in the vas deferens and epididymis tail was determined using fully automated analyzers. Serum levels of the three lipids, cytokines, and hormones were detected by ELISA. Pathological changes in the testicular tissue and epididymal fat were observed by H&E and immunofluorescence staining of the testicular tissue for superoxide dismutase (SOD) and 4‐hydroxynonenal (4‐HNE). Changes in the levels of MAPK pathway proteins in the testicular cells were detected by western blotting. Result. GSDF intervention significantly reduced the body weight, renal index, and white fat in obese mice, while increasing the testicular organ index. GSDF intervention significantly reduced serum total cholesterol (TC), triglyceride (TG), and low‐density lipoprotein cholesterol (LDL‐C) levels in mice compared to the HFD group, thereby improving hyperlipidemia. Simultaneously, the serum cytokine IL‐4 level was increased, IL‐6 level was significantly reduced, testosterone (T) hormone level was significantly increased, and follicle‐stimulating hormone (FSH) and luteinizing hormone (LH) levels were significantly reduced in the GSDF‐treated mice. The viability, survival rate, and density of spermatozoa in the treated groups significantly improved. Testicular interstitial cell vacuolization and collagen fibrosis improved, spermatogonia were aligned, and epididymal fat cell hypertrophy and vacuolization were suppressed. In the GSDF treatment group, SOD levels increased significantly, whereas 4‐HNE levels decreased, with the most evident effect observed in the medium‐dose group. GSDF ameliorated metabolic disorders in obese mice by regulating the p‐JNK/p‐p38MAPK pathway. Conclusion. GSDF ameliorated spermatogenic potential in obese mice by regulating the MAPK signaling pathway. Thus, GSDF may be an effective lipid‐lowering agent for improving the reproductive potential of obese mice.

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Combination of Panax ginseng and Diospyros kaki Leaf Inhibits White Adipocyte Differentiation and Browning Process through AMP-Activated Protein Kinase (AMPK) Activation In Vitro and In Vivo

Cited by 3 publications

References 32 publications

Rabbit Meat Extract Induces Browning in 3T3−L1 Adipocytes via the AMP−Activated Protein Kinase Pathway

Rabbit Meat Extract Induces Browning in 3T3−L1 Adipocytes via the AMP−Activated Protein Kinase Pathway

Discussion on the treatment of diabetic kidney disease based on the “gut–fat–kidney” axis

Ginseng Soluble Dietary Fiber Enhances Spermatogenic Potential in Obese Mice via the MAPK Signaling Pathway

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