Combination of platelet rich plasma and stromal vascular fraction on the level of vascular endothelial growth factor in rat subjects experiencing deep dermal burn injury

Abstract

Background The healing process of burns includes coagulation, inflammation, and remodeling. Vascular endothelial growth factor (VEGF) is involved throughout this healing process. Stem cells from the platelet-rich plasma (PRP) with stromal vascular fraction (SVF) can increase concentrations of growth factors, including VEGF. This is expected to accelerate burn healing. The aim of this study was to determine the effect of a combination of PRP and SVF on VEGF levels in a rats model of deep dermal burn wound healing. Materials and methods This is an experimental research study in rats using a post-test control group design with 4 groups: A) control, B) Vaseline, C) topical PRP and SVF, and D) PRP and SVF injection. Burn wounds were induced according to the modified Guo method. Results In a rats model of deep dermal wound healing, topical Vaseline significantly increased serum VEGF compared to control. Topical application and injection of stem cells also significantly increased serum VEGF compared to control and Vaseline. The VEGF concentration was significantly higher following injection of PRP and SVF, suggesting that the injection route is more effective at increasing VEGF levels compared to the topical application of stem cells. Conclusion The combination of PRP and SVF, either by injection or topical application, can increase VEGF levels during the healing process from deep dermal burns.

Systemic lidocaine administration influences NF-kβ gene expression, NF-kβ and TNF- α protein levels on BALB/c mice with musculoskeletal injury

“…Protein levels of NF-kβ and TNF-α were detected by MPO Sandwich ELISA with Catalog No. Ls-F12149 and LSF12798 were purchased from Life Span Bio Sciences, Inc. (Seattle, USA) according to the manufacturer's instructions [ [21] , [22] , [23] , [24] ].…”

The Combination of Stromal Vascular Fraction Cells and Platelet-Rich Plasma Reduces Malondialdehyde and Nitric Oxide Levels in Deep Dermal Burn Injury

“…[11][12][13] Platelet Rich Plasma (PRP) is known to play an important role in burn healing, mainly due to the many growth factors it contains. [14][15][16] Platelet-derived endothelial cell growth factor (PDGF) and insulin-like growth factor-I (IGF-I) are examples of growth factors that are present in PRP, inhibit the apoptotic pathway during cell turnover and facilitate different stages of wound healing. Both SVFs and PRP have the ability to accelerate epithelialization, induce angiogenesis, and stimulate fibroblasts.…”

“…This study is a continuation of the previous studies. 10,16,17 The results obtained from the previous studies which compared the local injection of SVFs, PRP, the combination of SVFs and PRP, and Vaseline (positive control) found that the combination of SVFs and PRP showed a better epithelialization result (complete closure on day 10) compared to SVFs group, PRP group, or Vaseline group alone. In addition, the formation of collagen was thicker, and the number of fibroblasts were higher in the combination group.…”

Analysis of CD4 and CD8 expression in multidrug-resistant tuberculosis infection with diabetes mellitus: An experimental study in mice

“…The cage was standard-shaped, cleaned regularly, and given lighting (12 h light/dark photoperiod) [ 28 , 29 ]. The laboratory animals were treated according to National Institutes of Health guidelines, and this work was carried out in line with the ARRIVE Guidelines for Reporting Animal Research [ [30] , [31] , [32] ].…”