Combination of polymer-bound charged groups and affinity ligands for extraction of enzymes by partitioning in aqueous two-phase systems

Cheng, Lüling; Joelsson, Monica; Johansson, Gillis

doi:10.1016/0021-9673(90)85016-o

Cited by 15 publications

(7 citation statements)

References 17 publications

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“…Similar relationships can be assumed to be important in the relative effectiveness of “affinity handles” developed for the purification of recombinant protein conjugates in ABSs by Veide and co-workers. − The addition of fused tryptophan-rich peptides to recombinant proteins enhanced their distribution to the PEG-rich phase of a PEG/phosphate ABS. Similarly, these results can explain the observed tendency for large hydrophobic aromatic compounds such as lignins, − biological pigments, and other dyes, including those used in group-specific affinity partitioning, , to partition to the PEG-rich phase of PEG/salt ABSs.…”

Section: Resultsmentioning

confidence: 71%

“…[64][65][66] The addition of fused tryptophan-rich peptides to recombinant proteins enhanced their distribution to the PEG-rich phase of a PEG/phosphate ABS. Similarly, these results can explain the observed tendency for large hydrophobic aromatic compounds such as lignins, [13][14][15] biological pigments, and other dyes, including those used in groupspecific affinity partitioning, 22,67 to partition to the PEGrich phase of PEG/salt ABSs.…”

Section: Hydrophobicitymentioning

confidence: 79%

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Solvent Properties of Aqueous Biphasic Systems Composed of Polyethylene Glycol and Salt Characterized by the Free Energy of Transfer of a Methylene Group between the Phases and by a Linear Solvation Energy Relationship

Willauer

Huddleston

Rogers

2002

Ind. Eng. Chem. Res.

105

139

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Aqueous biphasic systems (ABSs) composed of poly(ethylene glycol) (PEG) and salt have been examined as potential environmentally benign solvents for liquid/liquid extraction. These systems might also represent an alternative to traditional solvent/water systems used in quantitative structure-activity relationships (QSARs). For the application and design of these systems, it is important to have a thorough understanding of the nature of the solvent and its interactions with the solute, and thus, PEG/salt ABSs have been characterized to this end by a variety of methods. The relative hydrophobicities of several PEG/salt ABSs composed of different molecular weights of PEG (1000, 2000, and 3400) and a variety of inorganic salts [K 3 PO 4 , K 2 CO 3 , (NH 4 ) 2 -SO 4 , Li 2 SO 4 , MnSO 4 , ZnSO 4 , and NaOH] were measured by the free energy of transfer of a methylene group ∆G CH 2 . These results indicate that the relative hydrophobicity of a PEG/salt ABS is a function of only the degree of phase divergence of the biphasic system as expressed by the difference in polymer concentration between the phases [delta poly(ethylene glycol) (∆PEG), delta ethylene oxide monomer (∆EO)] or the tie line length (TLL). The distributions of a wide range of solutes differing in structure and functionality were also measured in PEG/salt ABSs, and the results were compared to the corresponding 1-octanol/water partition coefficients. These data were used to develop a linear free energy relationship (LFER) based on Abraham's generalized solvation equation, enabling a direct comparison to be made between the solvent properties of PEG/salt ABSs and those of traditional solvent/water systems used, for example, in the determination of log P. Similar comparisons are also enabled with the properties of certain aqueous micellar systems.

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Section: Resultsmentioning

confidence: 71%

Section: Hydrophobicitymentioning

confidence: 79%

Solvent Properties of Aqueous Biphasic Systems Composed of Polyethylene Glycol and Salt Characterized by the Free Energy of Transfer of a Methylene Group between the Phases and by a Linear Solvation Energy Relationship

Willauer

Huddleston

Rogers

2002

Ind. Eng. Chem. Res.

105

139

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“…The partition coefficient, K , was calculated as ratio of protein concentration in the top phase divided by protein concentration in the bottom phase. The presence of DEAE-dextran in system affected the partitioning of proteins (Cheng, 1990 Figure 2 shows the relationship of partition coefficient and pH value for six kinds of proteins when there was 1. 6 % (w /w) DEAE-dextran in systems.…”

Section: Materiat and Methodsmentioning

confidence: 99%

Partition of proteins in aqueous two-phase systems containing charged dextran or hydrophobic PEG

1993

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The electrochemical effect of a charged dextran derivative and the hydrophobic effect of hydrophobic chain PEG derivative on partitioning of six types of proteins in PEGjdextran aqueous two-phase systems were investigated. When 1. 6%(w/u)DEAE-dextran was present in the system ,the partition coefficient decreased quickly with increasing pH value; when 0. 3% (w/w )PEG pentadecanoic acid ester was present in the system. the partition coefficient of protein with strong hydrophobiclty was greatly increased. The experimental results show that the influence of hydrocarbon chain PEG derivative on partition coefficient is closely related to the hydrophobicity of proteins-

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“…Cheng et al (1990) investigated the extraction of glucose-6-phosphate dehydrogenase (G6PHD) in a PEG3400-Dextran T500 aqueous two-phase system with the addition of polymers carrying charged groups and affinity ligands. These polymers included DEAE-dextran, dextran sulfate, ligand-dextran, ligand-PEG, ligand-Ficoll and ligand-DEAE-dextran, where the ligands used were the Cibacron Blue F3G-A and Procion Yellow HE-3G.…”

Section: Triazine Dyes Bound To Polymersmentioning

confidence: 99%

Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems

Souza

Pontes

et al. 2003

Braz. arch. biol. technol.

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From analytical to commercial scale, aqueous two-phase systems have their application in the purification, characterization and study of biomaterials. In order to improve the selectivity of the systems, the biospecific affinity ligands were introduced. In the affinity partitioning aqueous two-phase system, have many enzymes been purified. This review discusses the partitioning of some enzymes in the affinity aqueous two-phase systems in regard to the different ligands, including reactive dyes, metal ions and other ligands. Some integration of aqueous two-phasesystem with other techniques for more effective purification of enzymes are also presented.

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Combination of polymer-bound charged groups and affinity ligands for extraction of enzymes by partitioning in aqueous two-phase systems

Cited by 15 publications

References 17 publications

Solvent Properties of Aqueous Biphasic Systems Composed of Polyethylene Glycol and Salt Characterized by the Free Energy of Transfer of a Methylene Group between the Phases and by a Linear Solvation Energy Relationship

Solvent Properties of Aqueous Biphasic Systems Composed of Polyethylene Glycol and Salt Characterized by the Free Energy of Transfer of a Methylene Group between the Phases and by a Linear Solvation Energy Relationship

Partition of proteins in aqueous two-phase systems containing charged dextran or hydrophobic PEG

Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems

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