Combination of <scp>CD</scp>160 and <scp>CD</scp>200 as a useful tool for differential diagnosis between chronic lymphocytic leukemia and other mature B‐cell neoplasms

Lesesve, J.‐F.; Tardy, Stéphanie; Frotscher, Birgit; Latger‐Cannard, Véronique; Feugier, Patrick; Bittencourt, Marcelo De Carvalho

doi:10.1111/ijlh.12315

Cited by 21 publications

(25 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A striking difference in CD200 expression on CLL versus MCL cells was found, with all 56 typical CLL samples positive for CD200 and all of the 6 MCL samples expressing little or no CD200, consistent with previous reports . The median % of cells positive for CD200 in our typical CLL cohort (99.5%) is very similar to the 94%, 96%, and 100% expression found in previous studies.…”

Section: Discussionsupporting

confidence: 90%

“…CD54 and CD160 were assessed because they have been proposed to distinguish between CLL and MCL . However, CD54 did not differ between CLL and MCL.…”

Section: Resultsmentioning

confidence: 99%

“…Although we have demonstrated that the atypical CLL cases are not MCL, the possibility that one or more of these cases may not be CLL cannot be excluded. However, marginal zone lymphoma seems unlikely, as a report on CD200 expression in mature B cell neoplasms found 4 cases of CD5+ marginal zone lymphoma all of which were negative for CD200 …”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

CD200 is a useful diagnostic marker for identifying atypical chronic lymphocytic leukemia by flow cytometry

Ting

Smith

Brown

et al. 2018

Int J Lab Hematology

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 90%

“…CD54 and CD160 were assessed because they have been proposed to distinguish between CLL and MCL . However, CD54 did not differ between CLL and MCL.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

CD200 is a useful diagnostic marker for identifying atypical chronic lymphocytic leukemia by flow cytometry

Ting

Smith

Brown

et al. 2018

Int J Lab Hematology

View full text Add to dashboard Cite

show abstract

“…Recently, several new markers which are considered to be commonly expressed by neoplastic CLL cells have been identified . Among them, CD200, a member of the Ig receptors membrane proteins superfamily, has been suggested to be rather specific for CLL . The present study aimed to analyze the role of CD200 in the diagnosis of CLL and whether this marker adds discriminant power to the MS.…”

Section: Discussionmentioning

confidence: 98%

“…Positivity of CD200 using MFIR values was also assessed. The positivity threshold was set up at 18 according to a prior study and was confirmed by receiver operating characteristics (ROC) curves.…”

Section: Methodsmentioning

confidence: 99%

CD200 is a useful marker in the diagnosis of chronic lymphocytic leukemia

Mora

Bosch

Cuellar

et al. 2018

Cytometry Part B Clinical

View full text Add to dashboard Cite

Background The diagnosis of CLL is supported by a typical morphology and immunophenotype and usually does not present difficulties. Nevertheless, some patients with CLL can show an atypical phenotype, this raising the possibility of a lymphoproliferative disorder other than CLL. It has been recently shown that the expression of CD200 could be a rather consistent marker for CLL. Methods The expression of CD200 was investigated in 120 consecutive patients with B‐cell chronic lymphoproliferative disorders (B‐CLPD) (65 cases diagnosed as typical CLL, 16 atypical CLL, and 39 non‐CLL before entering the study) by using multiparametric flow cytometry with four color combinations. CD200 was analyzed as percentage of positive cells (≥30%) and MFIR expression. ROC curves were used to determine the cut‐off for the CD200 MFIR. Matutes score (MS) was used as comparator. Results All 81 (100%) patients classified as CLL and 25 of 39 (64.1%) classified as non‐CLL expressed high CD200 expression (≥30%). CD200 expression showed a high sensitivity (100%) and a low specificity (35.9%), and the accuracy was similar to that of Matutes score markers (range: 79.2%–86.7%); except SmIg that was 59.1%. The addition of CD200 to the Matutes score correctly identified 74 of 81 (91.4%) CLL cases including 9 of 16 atypical CLL cases. As per non CLL cases, 37 of 39 (94.9%) were correctly diagnosed by the modified system. Altogether, CD200 improved the diagnostic accuracy of Matutes score from 86.7% to 92.5% (P < .01). Conclusion These results show that CD200 is a valuable, albeit not specific, CLL diagnostic marker. © 2018 International Clinical Cytometry Society

show abstract

Positive predictive value of CD200 positivity in the differential diagnosis of chronic lymphocytic leukemia

Sorigué

Magnano

Miljković

et al. 2019

Cytometry Part B Clinical

View full text Add to dashboard Cite

Background The role of CD200 in the differential diagnosis of chronic lymphocytic leukemia (CLL) and classical mantle cell lymphoma (MCL) is well established. Its role in the differential diagnosis of CLL and other lymphoproliferative disorders (LPD) is less clear, in particular its positive predictive value (PPV). Materials and methods We conducted a systematic review of the use of CD200 in the differential diagnosis of CLL, MCL, and other predominantly leukemic, typically CD103‐negative LPD. With the results, we then derived a curve to determine the PPV based on the prevalence of the disorders included in the differential diagnosis. Results Of 43 publications screened, 27 were included in the systematic review (5,764 patients). The median CD200 positivity rate in all studies and the percentage of CD200‐positive (pooled) patients was 100% and 95% (3,061/3,208) in CLL, 4 and 8% (86/1112) in MCL and 56 and 62% (425/689) in other LPD. Conclusion CD200 is suboptimal for the differential diagnosis of CLL and disorders other than nodal MCL.

show abstract

Combination of CD160 and CD200 as a useful tool for differential diagnosis between chronic lymphocytic leukemia and other mature B‐cell neoplasms

Abstract: CDs160/200 were associated with markers of the gold standard 'Matutes score' and could be useful markers to differentiate atypical CLL from other B-cell neoplasms in the absence of available biopsies or cytogenetics and molecular studies.

Cited by 21 publications

References 30 publications

CD200 is a useful diagnostic marker for identifying atypical chronic lymphocytic leukemia by flow cytometry

CD200 is a useful diagnostic marker for identifying atypical chronic lymphocytic leukemia by flow cytometry

CD200 is a useful marker in the diagnosis of chronic lymphocytic leukemia

Positive predictive value of CD200 positivity in the differential diagnosis of chronic lymphocytic leukemia

Contact Info

Product

Resources

About