Combination of stem cells from deciduous teeth and electroacupuncture for therapy in dogs with chronic spinal cord injury: A pilot study

Prado, Cristiane do; Fratini, Paula; Matias, Gustavo de Sá Schiavo; Bocabello, Renato Zonzini; Monteiro, Juliana; Santos, Célio J. dos; Joaquim, Jean Guilherme Fernandes; Giglio, Robson F.; Possebon, Fábio Sossai; Sakata, Stella; Miglino, María Angélica

doi:10.1016/j.rvsc.2019.01.011

Cited by 28 publications

(32 citation statements)

References 23 publications

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“…It seems that single-therapy do not have a sufficient effect on the SCI [35]. In recent years, several preclinical studies used combination of various treatments for SCI [36][37][38]. Based on our knowledge, no study has investigated the effect of combination of laser and hADSCs on the recovery after SCI.…”

Section: Introductionmentioning

confidence: 99%

Co-administration of human adipose-derived stem cells and low-level laser to alleviate neuropathic pain after experimental spinal cord injury

et al. 2019

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Background: Evidence has suggested that human adipose-derived stem cells (hADSCs) and low-level laser has neuroprotective effects on spinal cord injury (SCI). Therefore, the combined effect of the hADSCs and laser on neuregeneration and neuropathic pain after SCI was investigated. Methods: Forty-eight adult male Wistar rats with 200-250 g weight were used. Thirty minutes after compression, injury with laser was irritated, and 1 week following SCI, about 1 × 10 6 cells were transplanted into the spinal cord. Motor function and neuropathic pain were assessed weekly. Molecular and histological studies were done at the end of the fourth week. Results: The combined application of hADSCs and laser has significantly improved motor function recovery (p = 0.0001), hyperalgesia (p < 0.05), and allodynia (p < 0.05). GDNF mRNA expression was significantly increased in hADSCs and laser+hADSC-treated animals (p < 0.001). Finally, co-administration of hADSCs and laser has enhanced the number of axons around cavity more than other treatments (p < 0.001). Conclusions: The results showed that the combination of laser and ADSCs could significantly improve the motor function and alleviate SCI-induced allodynia and hyperalgesia. Therefore, using a combination of laser and hADSCs in future experimental and translational clinical studies is suggested.

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Section: Introductionmentioning

confidence: 99%

Co-administration of human adipose-derived stem cells and low-level laser to alleviate neuropathic pain after experimental spinal cord injury

et al. 2019

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“…There is equivocal evidence that electroacupuncture decreases the severity and duration of post-operative pain in dogs with IVDH (74,76). Electroacupuncture has also been combined with stem cell transplantation in a small group of dogs chronically (> 3 months) deep pain negative following acute IVDH (77). This pilot study showed these interventions were feasible and safe but case numbers in each treatment group were small.…”

Section: Electroacupuncturementioning

confidence: 91%

“…Since 2014, institutions in Brazil have reported seven trials testing safety or efficacy of various cell transplants (within the spinal cord or in the sub-arachnoid or epidural space) in small series of companion dogs, sometimes with concomitant spinal cord decompression, or in association with other alternative therapies such as electroacupuncture (77) or lowintensity electrical stimulation (81). The cells tested have been either autologous bone marrow mesenchymal stem cells (147), allogenic fetal bone marrow stem cells (148), allogenic canine adipose tissue-derived mesenchymal stem cells (81,149,150) or immature dental pulp stem cells (77,151).…”

Section: Cell Transplantation Within the Spinal Cord In Dogs With Natmentioning

confidence: 99%

Emerging and Adjunctive Therapies for Spinal Cord Injury Following Acute Canine Intervertebral Disc Herniation

2020

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Some dogs do not make a full recovery following medical or surgical management of acute canine intervertebral disc herniation (IVDH), highlighting the limits of currently available treatment options. The multitude of difficulties in treating severe spinal cord injury are well-recognized, and they have spurred intense laboratory research, resulting in a broad range of strategies that might have value in treating spinal cord-injured dogs. These include interventions that aim to directly repair the spinal cord lesion, promote axonal sparing or regeneration, mitigate secondary injury through neuroprotective mechanisms, or facilitate functional compensation. Despite initial promise in experimental models, many of these techniques have failed or shown mild efficacy in clinical trials in humans and dogs, although high quality evidence is lacking for many of these interventions. However, the continued introduction of new options to the veterinary clinic remains important for expanding our understanding of the mechanisms of injury and repair and for development of novel and combined strategies for severely affected dogs. This review outlines adjunctive or emerging therapies that have been proposed as treatment options for dogs with acute IVDH, including discussion of local or lesion-based approaches as well as systemically applied treatments in both acute and subacute-to-chronic settings. These interventions include low-level laser therapy, electromagnetic fields or oscillating electrical fields, adjunctive surgical techniques (myelotomy or durotomy), systemically or locally-applied hypothermia, neuroprotective chemicals, physical rehabilitation, hyperbaric oxygen therapy, electroacupuncture, electrical stimulation of the spinal cord or specific peripheral nerves, nerve grafting strategies, 4-aminopyridine, chondroitinase ABC, and cell transplantation.

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“…SCI is a severe traumatic central nervous system disease resulting in the damage of sensory and motor functions. It has been demonstrated by recent studies that dental MSCs could facilitate functional improvement after SCI in animal models [ 108 – 111 ]. For instance, SHED-CM loaded in collagen hydrogel was injected into the injury site and gained higher Basso, Beattie, and Bresnahan (BBB) scores which suggested that this new cell-free therapeutic approach is conducive to sensory and motor function recovery of SCI [ 110 ].…”

Section: Dental Msc-based Therapy For Nondental Diseasesmentioning

confidence: 99%

“…Peripheral nerve injury following traumatic accidents or surgical complications is a severe clinical problem resulting in sensory disturbances, paralysis, and locomotive disability. Because dental MSCs present a great privilege in neurogenic differentiation, they are a hopeful cell source to treat injured peripheral nerves, like the sciatic nerve and SLN [ 111 – 113 ]. For example, the sciatic nerve could be regenerated and repaired after hDPSC implantation or exosome derived from GMSC transplantation in a rat model with sciatic nerve defects [ 112 , 113 ].…”

Section: Dental Msc-based Therapy For Nondental Diseasesmentioning

confidence: 99%

Dental Tissue-Derived Human Mesenchymal Stem Cells and Their Potential in Therapeutic Application

Gan

Liu

Cui

et al. 2020

Stem Cells International

100

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Human mesenchymal stem cells (hMSCs) are multipotent cells, which exhibit plastic adherence, express specific cell surface marker spectrum, and have multi-lineage differentiation potential. These cells can be obtained from multiple tissues. Dental tissue-derived hMSCs (dental MSCs) possess the ability to give rise to mesodermal lineage (osteocytes, adipocytes, and chondrocytes), ectodermal lineage (neurocytes), and endodermal lineages (hepatocytes). Dental MSCs were first isolated from dental pulp of the extracted third molar and till now they have been purified from various dental tissues, including pulp tissue of permanent teeth and exfoliated deciduous teeth, apical papilla, periodontal ligament, gingiva, dental follicle, tooth germ, and alveolar bone. Dental MSCs are not only easily accessible but are also expandable in vitro with relative genomic stability for a long period of time. Moreover, dental MSCs have exhibited immunomodulatory properties by secreting cytokines. Easy accessibility, multi-lineage differentiation potential, and immunomodulatory effects make dental MSCs distinct from the other hMSCs and an effective tool in stem cell-based therapy. Several preclinical studies and clinical trials have been performed using dental MSCs in the treatment of multiple ailments, ranging from dental diseases to nondental diseases. The present review has summarized dental MSC sources, multi-lineage differentiation capacities, immunomodulatory features, its potential in the treatment of diseases, and its application in both preclinical studies and clinical trials. The regenerative therapeutic strategies in dental medicine have also been discussed.

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Combination of stem cells from deciduous teeth and electroacupuncture for therapy in dogs with chronic spinal cord injury: A pilot study

Cited by 28 publications

References 23 publications

Co-administration of human adipose-derived stem cells and low-level laser to alleviate neuropathic pain after experimental spinal cord injury

Co-administration of human adipose-derived stem cells and low-level laser to alleviate neuropathic pain after experimental spinal cord injury

Emerging and Adjunctive Therapies for Spinal Cord Injury Following Acute Canine Intervertebral Disc Herniation

Dental Tissue-Derived Human Mesenchymal Stem Cells and Their Potential in Therapeutic Application

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