Combination of thidiazuron and 2-isopentenyladenine promotes highly efficient adventitious shoot regeneration from cotyledons of mature sunflower (Helianthus annuus L.) seeds

Sujatha, M.; Vijay, Sheri; Singareddy, Vasavi; Rao, S. Chander

doi:10.1007/s11240-012-0202-1

Cited by 23 publications

(20 citation statements)

References 56 publications

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“…Hence, the adventitious shoot buds of N. cadamba developed de novo and from the explant epidermal cells as a result of mitotic activity of the epidermal cells in response to appropriate hormone concentrations, but not from preexisting meristems (vascular elements). This emergence is similar to that of the sunflower (Sujatha et al 2012), but different from Centaurea ultreiae, in which meristematic cells arise from the original vascular elements (Mallon et al 2011).…”

Section: Histological Observationmentioning

confidence: 59%

Direct adventitious shoot organogenesis and plant regeneration from cotyledon explants in Neolamarckia cadamba

Huang

Ouyang

et al. 2014

Plant Biotechnology

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Neolamarckia cadamba is a fast-growing and deciduous tropical hardwood with anatomical, morphological, and chemical characteristics that make it suitable for building materials, pulp production, and medicine raw materials. In this study, a protocol for direct adventitious shoot organogenesis and plant regeneration from the aseptic cotyledons of N. cadamba was established. The cotyledons with petioles from 3-week-old seedlings were used for adventitious shoot induction in DCR medium containing 22.20 µM 6-benzyladenine (BA) and 0.27 µM α-naphthaleneacetic acid (NAA). The frequency of adventitious shoot induction was 54.2%. Micro-shoots were then transferred to MS medium containing 4.44 µM BA and 0.25 µM indole-3-butyric acid (IBA) for shoot propagation. Available shoots per explant reached 5.9. The highest rooting percentage (98.3%) was obtained when shoots were transferred to half-strength MS medium supplemented with 0.27 µM NAA and 0.25 µM IBA. The rooted plantlets could be successfully acclimatized to a greenhouse with more than 95% survival, and the regenerated plants showed the same morphological characteristics as those of the control plants in fields. Histological observations revealed that the adventitious shoots only originated from the epidermal tissue around the edge of the cut zone of the cotyledonary petiole.

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Section: Histological Observationmentioning

confidence: 59%

Direct adventitious shoot organogenesis and plant regeneration from cotyledon explants in Neolamarckia cadamba

Huang

Ouyang

et al. 2014

Plant Biotechnology

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“…As the RHA280 line that was used for most of the research described here was generally more responsive to shoot induction than the single genotype evaluated in that study, the RHA280 line may show an enhanced shoot production that is not typical for all sunflower lines. Genetic control of shoot organogenesis (Sarrafi et al 1996) as well as variation in regeneration among genotypes (Sujatha et al 2012b) has been previously reported for sunflower cotyledon tissues. Shoot formation was obtained from preconditioned leaves of all seven genotypes evaluated in the present study, although the efficiency differed considerably (Table 3), further validating a genetic influence on shoot formation in sunflower tissues.…”

Section: Discussionmentioning

confidence: 77%

“…More recently, genetic transformation of cotyledonary tissues using Agrobacterium has also been reported (Sujatha et al 2012a). Shoot formation in sunflower has primarily been obtained using the shoot apex (Paterson 1984), embryo axis or preferably cotyledon explants from seeds or seedlings (Power 1987;Knittel et al 1991;Sujatha et al 2012b). Shoot buds have been obtained from expanding leaves of cultured shoot apices following culture of the leaves on a medium containing benzylaminopurine (BA) (Konov et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Sunflower (Helianthus annuus L.) organogenesis from primary leaves of young seedlings preconditioned by cytokinin

Zhang

Finer

2015

Plant Cell Tiss Organ Cult

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Leaf tissue is widely used for regeneration of many different plants, but an efficient regeneration method using leaf tissue of sunflower is not yet available. In this report, adventitious shoots were obtained from primary leaves of 7-day-old sunflower seedlings. If seeds were germinated on a medium without cytokinin, only 25 % of the leaf explants produced shoots after 3 weeks of culture on a shoot induction medium (SIM) containing 1.5 mg L -1 benzylaminopurine (BA), giving an average of three shoot primordia per leaf. Shoot induction was enhanced up to sixfold when seeds were germinated on media containing BA or 6-(c, c-dimethylallylamino)purine at 5 mg L -1 and higher, or kinetin at 15 mg L -1 and above, with an average of 12-19 shoot primordia per leaf. Shoots further developed and elongated following transfer to a medium containing 0.1 mg L -1 gibberellic acid. The highest number of developed shoots was obtained from leaves preconditioned with 5 mg L -1 BA, with 1.9 developed shoots per explant. Histological analysis of leaves from seedlings germinated on a medium containing 5 mg L -1 BA revealed increased cell division primarily in the leaf surface tissue on the adaxial side of the mid vein. After the leaves were excised and plated on SIM, these cells continued to divide and differentiate to form adventitious shoots, suggesting that the cytokinin-containing germination medium preconditioned leaves for shoot organogenesis. An in vitro micrografting approach was developed using the elongated shoots as scions, and 57 % of grafted shoots survived to form plants.

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“…As a result of inefficiencies in sunflower regeneration, transgenic approaches for basic research and improvement of this important oilseed crop have been somewhat limited. Thus far, shoot organogenesis in sunflower has been reported using shoot tips (Paterson 1984), cotyledon tissues from mature embryos (Power 1987;Dhaka and Kothari 2002;Sujatha et al 2012) and germinated seedlings (Knittel et al 1991;Ceriani et al 1992;Baker et al 1999), immature embryos (Müller et al 2001), and leaf tissues (Konov et al 1998;Zhang and Finer 2015). Due to year-round availability and high-shooting response from mature embryos and seedling materials, these tissue sources are preferred over immature embryos for sunflower regeneration.…”

Section: Introductionmentioning

confidence: 99%

“…Due to year-round availability and high-shooting response from mature embryos and seedling materials, these tissue sources are preferred over immature embryos for sunflower regeneration. With continuous culture on cytokinin-containing medium, cotyledonary tissues from dry mature seeds were more responsive to shoot induction than cotyledons from germinated seeds (Power 1987;Sujatha et al 2012). The cytokinin 6-benzylaminopurine (BA) was used most often for studies on sunflower shoot production (Paterson 1984;Power 1987;Knittel et al 1991;Dhaka and Kothari 2002), as it was more effective than other cytokinins (Paterson 1984).…”

Section: Introductionmentioning

confidence: 99%

Use of cytokinin pulse treatments and micrografting to improve sunflower (Helianthus annuus L.) plant recovery from cotyledonary tissues of mature seeds

Zhang

Finer

2016

In Vitro Cell.Dev.Biol.-Plant

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Regeneration of sunflower plants from tissue culture can be problematic due to inefficiencies in shoot elongation and subsequent rooting. To minimize exposure to cytokinins, which can negatively impact shoot elongation and rooting, a cytokinin pulse treatment was evaluated by placing cotyledonary tissues from sunflower seeds onto a shoot induction medium containing 1.5 mg L −1 6-benzylaminopurine and 0.2 mg L −1 1-naphthaleneacetic acid for 0, 2, 4, 8, 12, and 16 d, prior to transfer to an elongation medium containing 0.1 mg L −1 gibberellic acid. The 16-d pulse treatment gave the highest numbers of shoot primordia, but shoot development was minimal and only 0.4 well-developed shoots could be obtained per explant. The 4-d pulse treatment followed by 14 d of culture on elongation medium led to the production of 5.5 well-developed shoots per explant. For plant recovery, well-developed shoots obtained with the 4-d pulse treatment followed by a 14-d elongation treatment were micrografted onto in vitro seedlings, with graft survival of over 60%. Short pulse treatments (4-and 8-d), followed by culture on elongation medium, generated well-developed shoots that seemed to be more responsive to micrografting than shoots obtained with the 16-d pulse treatment. The use of the 4-d pulse treatment with micrografting of well-developed shoots significantly improved the efficiency of whole-plant recovery of sunflower.

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Combination of thidiazuron and 2-isopentenyladenine promotes highly efficient adventitious shoot regeneration from cotyledons of mature sunflower (Helianthus annuus L.) seeds

Cited by 23 publications

References 56 publications

Direct adventitious shoot organogenesis and plant regeneration from cotyledon explants in Neolamarckia cadamba

Direct adventitious shoot organogenesis and plant regeneration from cotyledon explants in Neolamarckia cadamba

Sunflower (Helianthus annuus L.) organogenesis from primary leaves of young seedlings preconditioned by cytokinin

Use of cytokinin pulse treatments and micrografting to improve sunflower (Helianthus annuus L.) plant recovery from cotyledonary tissues of mature seeds

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