Combination Therapy of Stem Cell-derived Exosomes and Biomaterials in the Wound Healing

Golchin, Ali; Shams, Forough; Basiri, Arefeh; Ranjbarvan, Parviz; Kiani, Samaneh; Sarkhosh-Inanlou, Roya; Ardeshirylajimi, Abdolreza; Aziz, Shiva Gholizadeh‐Ghaleh; Sadigh, Sanaz; Rasmi, Yousef

doi:10.1007/s12015-021-10309-5

Cited by 36 publications

(27 citation statements)

References 149 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, there are some controversies regarding the efficacy of stem cells in clinical trials [ 6 ]. Thus, interdisciplinary combination approaches such as exosomes, biomaterials, and biological engineering are needed to develop drug delivery systems or stem cell therapies to treat chronic wounds [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Dual CXCR4/IL-10 Gene-Edited Human Amniotic Mesenchymal Stem Cells Exhibit Robust Therapeutic Properties in Chronic Wound Healing

Han

Chae

Kim

2022

IJMS

View full text Add to dashboard Cite

Although stem cells have attracted attention as a novel therapeutic solution for tissue regeneration, their minimal efficacy remains controversial. In the present study, we aimed to investigate the enhanced therapeutic property of CXCR4/IL-10 dual angiogenic/anti-inflammatory gene knock-in amniotic mesenchymal stem cells (AMM) in a wound-healing model. Dual CXCR4 and IL-10 genes were inserted into the AMM genome using transcription-activator-like effector nuclease (TALEN). Matrigel tube formation and anti-inflammatory effects were assessed in vitro, and efficacy was tested in vivo in a diabetic wound-healing model. CXCR4/IL-10-expressing amniotic MSCs (AMM/CI) strongly expressed CXCR4 and IL-10 genes and robustly promoted tube formation and anti-inflammatory potential. AMM/CI transplantation resulted in accelerated wound healing, as well as high engraftment and re-epithelialization potential. Transplanted AMM/CI also exhibited high angiogenic and decreased pro-inflammatory gene expression in the wound tissue, indicating direct therapeutic effects on wound healing. Taken together, these data indicate that dual angiogenic/anti-inflammatory gene knock-in may be a novel approach to enhance the therapeutic effects of stem cells, and transplantation of AMM/CI can be an alternative therapeutic option in chronic wound healing.

show abstract

Section: Introductionmentioning

confidence: 99%

Dual CXCR4/IL-10 Gene-Edited Human Amniotic Mesenchymal Stem Cells Exhibit Robust Therapeutic Properties in Chronic Wound Healing

Han

Chae

Kim

2022

IJMS

View full text Add to dashboard Cite

show abstract

“…Short half-time and rapid clearance in vivo have been the challenges for injecting free sEVs in practical applications ( Golchin et al, 2022 ). Due to high designability of biomaterials, researchers have combined sEVs with innovatively designed biomaterials to prolong the retention time to increase their utilization, and achieve on-demand release in vivo ( Las Heras et al, 2020 ).…”

Section: Application Avenues Of Msc-sevs On Woundsmentioning

confidence: 99%

Small extracellular vesicles from mesenchymal stem cells: A potential Weapon for chronic non-healing wound treatment

Wei

Liu

et al. 2023

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Chronic non-healing wounds have posed a severe threat to patients mentally and physically. Behavior dysregulation of remaining cells at wound sites is recognized as the chief culprit to destroy healing process and hinders wound healing. Therefore, regulating and restoring normal cellular behavior is the core of chronic non-healing wound treatment. In recent years, the therapy with mesenchymal stem cells (MSCs) has become a promising option for chronic wound healing and the efficacy has increasingly been attributed to their exocrine functions. Small extracellular vesicles derived from MSCs (MSC-sEVs) are reported to benefit almost all stages of wound healing by regulating the cellular behavior to participate in the process of inflammatory response, angiogenesis, re-epithelization, and scarless healing. Here, we describe the characteristics of MSC-sEVs and discuss their therapeutic potential in chronic wound treatment. Additionally, we also provide an overview of the application avenues of MSC-sEVs in wound treatment. Finally, we summarize strategies for large-scale production and engineering of MSC-sEVs. This review may possibly provide meaningful guidance for chronic wound treatment with MSC-sEVs.

show abstract

“…As a new dressing, a hydrogel is a three-dimensional structure formed by physical or chemical crosslinking between hydrophilic polymer chains ( Zhao Y. et al, 2021 ; Ma and Wu, 2022 ; Safari et al, 2022 ). Hydrogels can load EdSC by absorbing a large amount of solution containing EdSC because of the hydrophilic functional groups in polymers ( Xu et al, 2018 ; Golchin et al, 2022 ). Applying the EdSC-loaded hydrogel dressing to the wound bed, the hydrogel network can control the release concentration and time of EdSC and increase the moisture content of the wound ( Kim et al, 2017 ; Shafei et al, 2020 ).…”

Section: A Promising Therapeutics Exosome Derived From Stem Cell For ...mentioning

confidence: 99%

Exosome derived from stem cell: A promising therapeutics for wound healing

Liu

Sun

et al. 2022

Front. Pharmacol.

View full text Add to dashboard Cite

A wound occurs when the epidermis and dermis of the skin are damaged internally and externally. The traditional wound healing method is unsatisfactory, which will prolong the treatment time and increase the treatment cost, which brings economic and psychological burdens to patients. Therefore, there is an urgent need for a new method to accelerate wound healing. As a cell-free therapy, exosome derived from stem cell (EdSC) offers new possibilities for wound healing. EdSC is the smallest extracellular vesicle secreted by stem cells with diameters of 30–150 nm and a lipid bilayer structure. Previous studies have found that EdSC can participate in and promote almost all stages of wound healing, including regulating inflammatory cells; improving activation of fibroblasts, keratinocytes, and endothelial cells; and adjusting the ratio of collagen Ⅰ and Ⅲ. We reviewed the relevant knowledge of wounds; summarized the biogenesis, isolation, and identification of exosomes; and clarified the pharmacological role of exosomes in promoting wound healing. This review provides knowledge support for the pharmacological study of exosomes.

show abstract

Combination Therapy of Stem Cell-derived Exosomes and Biomaterials in the Wound Healing

Cited by 36 publications

References 149 publications

Dual CXCR4/IL-10 Gene-Edited Human Amniotic Mesenchymal Stem Cells Exhibit Robust Therapeutic Properties in Chronic Wound Healing

Dual CXCR4/IL-10 Gene-Edited Human Amniotic Mesenchymal Stem Cells Exhibit Robust Therapeutic Properties in Chronic Wound Healing

Small extracellular vesicles from mesenchymal stem cells: A potential Weapon for chronic non-healing wound treatment

Exosome derived from stem cell: A promising therapeutics for wound healing

Contact Info

Product

Resources

About