Combination therapy with doxorubicin-loaded galactosylated poly(ethyleneglycol)-lithocholic acid to suppress the tumor growth in an orthotopic mouse model of liver cancer

Singh, Bijay; Jang, Yoonjeong; Maharjan, Sushila; Kim, Hyeon-Jeong; Lee, Ah Young; Kim, Sanghwa; Gankhuyag, Nomundelger; Yang, Myeon‐Sik; Choi, Yun‐Jaie; Cho, Myung‐Haing; Cho, Chong‐Su

doi:10.1016/j.biomaterials.2016.11.040

Cited by 39 publications

(17 citation statements)

References 45 publications

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“…According to our results, several studies have reported an apoptotic response after incubation with DOX covalently bonded to different types of iron oxide magnetic nanoparticle [38,39]. However, there are very few studies related to mitotic catastrophe for DOX-loaded nanoparticles [40]. It is important to note that, at least to our knowledge, this is the first time that an iron oxide nanoparticle DOX loading covalently has been described to be capable of inducing cell death at the same time by both the mechanisms of apoptosis and mitotic catastrophe.…”

Section: Discussionsupporting

confidence: 58%

Tailored Functionalized Magnetic Nanoparticles to Target Breast Cancer Cells Including Cancer Stem-Like Cells

Lázaro-Carrillo

Calero

Aires

et al. 2020

Cancers

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Nanotechnology-based approaches hold substantial potential to avoid chemoresistance and minimize side effects. In this work, we have used biocompatible iron oxide magnetic nanoparticles (MNPs) called MF66 and functionalized with the antineoplastic drug doxorubicin (DOX) against MDA-MB-231 cells. Electrostatically functionalized MNPs showed effective uptake and DOX linked to MNPs was more efficiently retained inside the cells than free DOX, leading to cell inactivation by mitotic catastrophe, senescence and apoptosis. Both effects, uptake and cytotoxicity, were demonstrated by different assays and videomicroscopy techniques. Likewise, covalently functionalized MNPs using three different linkers—disulfide (DOX-S-S-Pyr, called MF66-S-S-DOX), imine (DOX-I-Mal, called MF66-I-DOX) or both (DOX-I-S-S-Pyr, called MF66-S-S-I-DOX)—were also analysed. The highest cell death was detected using a linker sensitive to both pH and reducing environment (DOX-I-S-S-Pyr). The greatest success of this study was to detect also their activity against breast cancer stem-like cells (CSC) from MDA-MB-231 and primary breast cancer cells derived from a patient with a similar genetic profile (triple-negative breast cancer). In summary, these nanoformulations are promising tools as therapeutic agent vehicles, due to their ability to produce efficient internalization, drug delivery, and cancer cell inactivation, even in cancer stem-like cells (CSCs) from patients.

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Section: Discussionsupporting

confidence: 58%

Tailored Functionalized Magnetic Nanoparticles to Target Breast Cancer Cells Including Cancer Stem-Like Cells

Lázaro-Carrillo

Calero

Aires

et al. 2020

Cancers

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“…Electron microscopy images showed the veritable size of a single particle in a dried state (Mcallister et al 2016). In contrast, the size measured by dynamic light scattering technique was a hydrated state value of spherical particles, reflecting a single particle or aggregates (Zhong and Jin 2009;Singh et al 2017).…”

Section: Morphologymentioning

confidence: 96%

Preparation, characterization, and evaluation of azoxystrobin nanosuspension produced by wet media milling

et al. 2018

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To improve the bioavailability of the poorly water-soluble fungicide, an azoxystrobin nanosuspension was prepared by the wet media milling method. Due to their reduced mean particle size and polydispersity index, 1-Dodecanesulfonic acid sodium salt and polyvinylpyrrolidone K30 were selected from six conventional surfactants, the content only accounting for 15% of the active compound. The mean particle size, polydispersity index, and potential of the nanosuspension were determined to be 238.1 ± 1.5 nm, 0.17 ± 0.02 and − 31.8 ± 0.3 mV, respectively. The lyophilized nanosuspension mainly retained crystalline state, with only a little amorphous content as determined by powder X-ray diffraction. Compared to conventional fungicide formulations, the nanosuspension presented an increased retention volume and a reduced contact angle, indicating enhanced wettability and adhesion. In addition, the azoxystrobin nanosuspension showed the highest antifungal activity, with a medial lethal concentration of 1.4243 μg/mL against Fusarium oxysporum. In optical micrographs, hyphal deformations of thinner and intertwined hyphae were detected in the exposed group. Compared to the control group, the total soluble protein content, superoxide dismutase, and catalase activities were initially increased and then decreased with prolonged exposure time. The azoxystrobin nanosuspension reduced the defensive antioxidant capability of Fusarium oxysporum and resulted in the generation of excessive reactive oxygen species. This study provides a novel method for preparing nanosuspension formulation of poorly soluble antifungal agents to enhance the biological activity and decrease the negative environmental impact.

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“…By contrast, surfactants adsorbed on the surface of the nanoparticles are dissolved or swollen in aqueous media, such that the hydrodynamic diameter represented by DLS is larger than that observed from electron microscopy images . In addition, if the particles are agglomerated, the size measured by DLS would be also larger than the SEM and TEM results, because larger particles may dominate the light scattering signal and mask the presence of smaller particles during DLS measurement …”

Section: Resultsmentioning

confidence: 93%

Improving abamectin bioavailability via nanosuspension constructed by wet milling technique

Cui

Gao

et al. 2019

Pest Management Science

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BACKGROUND Poorly water‐soluble and photosensitive pesticide compounds are difficult to be formulated as environmentally friendly formulations with high efficacy. Conventional wettable powder, emulsifiable concentrate and emulsion in water have disadvantages of dust drift, overuse of organic solvent and low efficacy. Therefore, there is an urgent need to construct a novel formulation to improve the bioavailability of pesticides. RESULTS An abamectin nanosuspension was developed using a wet‐milling method combined with orthogonal experimental design. The average particle sizes of the abamectin nanosuspension measured by dynamic light scattering, scanning electron microscope and transmission electron microscope were 233, 90 and 140 nm, respectively. The zeta potential and sliding angle on cabbage leaves were −36.9 mV and 62°. Retention and anti‐photolysis were around 1.5 and 1.6 times those of emulsions in water. Furthermore, the biological activity of the nanosuspension towards diamondback moths was approximately twice that of conventional formulations. CONCLUSION This study provides an easy and scalable technique for constructing pesticide nanosuspensions. The preparation and composition of the nanosuspension avoid the use of organic solvents. Application of the highly effective nanoformulation will significantly enhance pesticide efficacy, and reduce the dosage and environmental pollution of the pesticide. © 2019 Society of Chemical Industry

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Combination therapy with doxorubicin-loaded galactosylated poly(ethyleneglycol)-lithocholic acid to suppress the tumor growth in an orthotopic mouse model of liver cancer

Cited by 39 publications

References 45 publications

Tailored Functionalized Magnetic Nanoparticles to Target Breast Cancer Cells Including Cancer Stem-Like Cells

Tailored Functionalized Magnetic Nanoparticles to Target Breast Cancer Cells Including Cancer Stem-Like Cells

Preparation, characterization, and evaluation of azoxystrobin nanosuspension produced by wet media milling

Improving abamectin bioavailability via nanosuspension constructed by wet milling technique

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