Combination Therapy with Taurine, Epigallocatechin Gallate and Genistein for Protection against Hepatic Fibrosis Induced by Alcohol in Rats

Zhuo, Lang; Liao, Ming; Li, Zheng; He, Miao; Huang, Quanfang; Wei, Ling; Huang, Renbin; Zhang, Shijun; Lin, Xiaobo

doi:10.1248/bpb.b12-00548

Cited by 16 publications

(12 citation statements)

References 31 publications

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“…In all nicotine + curcumin groups, there was a significant decrease in serum total antioxidant levels in comparison to the nicotine control group. The results of the study by Zhuo et al showed that total antioxidant level was reduced significantly in smokers in proportion to nonsmokers; which is consistent with the results of a similar study 35 . It seems that the reduction of total antioxidant capacity in plasma of smokers has led to an increase in the production of free radicals 36 .…”

Section: Discussionsupporting

confidence: 81%

Toxicological evaluation of the nicotine on CA1 and protective effects of curcuma longa: An experimental study

et al. 2019

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Introduction: Nicotine is the most important alkaloid compound in tobacco and is a major risk factor in the development of functional disorders of several organ systems. Some plants produce Curcuma longa (curcumin), which has antioxidant and neuro-protective properties. Methods: This study was designed to evaluate the therapeutic effects of curcumin against nicotine injury on the hippocampus CA1 region of rats. In this study, 48 male rats were randomly assigned to eight groups: Normal control (saline) group, Nicotine control group (0.5 mg/kg); Curcumin groups (10, 30, and 60 mg/kg) and Nicotine + Curcumin groups (10, 30, and 60 mg/kg). Treatments were administered intra-peritoneally daily for 28 days. Golgi staining technique investigated the number of dendritic spines. Cresyl violet staining method was used to determine the number of neurons in the hippocampal region CA1. Griess technique was assessed to determine serum nitrite oxide levels. Also, the ferric reducing/antioxidant power (FRAP) method was applied to determine the total antioxidant capacity. Results: Nicotine administration significantly increased the nitrite oxide level and decreased total antioxidant capacity. The number of neuronal dendritic spines, as well as neurons per se, also decreased, compared to the control group (p < 0.01). In all the members of the Curcumin and Nicotine + Curcumin groups, the number of neurons, neuronal dendritic spines and total antioxidant capacity increased significantly compared to the nicotine control group, while nitrite oxide level decreased significantly compared to the nicotine control group (p < 0.01). Conclusions: It seems that Curcumin administration improved hippocampal CA1 region injury in rats caused by Nicotine.

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Section: Discussionsupporting

confidence: 81%

Toxicological evaluation of the nicotine on CA1 and protective effects of curcuma longa: An experimental study

et al. 2019

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“…Nicotine can stimulate nicotinic receptors in the brain and increase glutamate release and N-Methyl-D-aspartic acid (NMDA) activation. The activation of NMDA may increase the formation of nitric oxide in the hippocampus (36). The results of a study by Keser et al revealed that exposure to nicotine may increase the activity of nitrite oxide in the frontal cortex in the mouse brain.…”

Section: Discussionmentioning

confidence: 99%

Effect of curcumin on hippocampus dentate gyrus injury induced by nicotine in rats

Jalili

Rodsari

Roshankhah

et al. 2019

J Herbmed Pharmacol

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Introduction: Nicotine is the most important alkaloid compound in tobacco and is a major risk factor in the development of functional disorder of several organ systems. Some plants produce Curcumin, which has antioxidant and neuroprotective properties. This study was designed to evaluate the therapeutic effects of curcumin against nicotine injury on the hippocampus CA1 region of rats. Methods: In this study, 48 male Wistar rats were randomly assigned to eight groups: Normal control (saline) group, Nicotine control group (0.5 mg/kg); Curcumin groups (10, 30, and 60 mg/kg) and Nicotine + Curcumin groups (10, 30, and 60 mg/kg). Treatments were administered intraperitoneally daily for 28 days. Golgi staining technique investigated the number of dendritic spines. Cresyl violet staining method was used to determine the number of neurons in hippocampal region CA1. Griess technique was assessed to determine serum nitrite oxide level. Also, the Ferric reducing/antioxidant power (FRAP) method was applied to determine the total antioxidant capacity. Results: Nicotine administration significantly increased nitrite oxide level and decreased total antioxidant capacity as well as the number of neuronal dendritic spines and neurons compared to the normal control group (P < 0.01). In all Curcumin and Nicotine + Curcumin groups, the number of neurons, neuronal dendritic spines, and total antioxidant capacity increased significantly compared to the nicotine control group, while nitrite oxide level decreased significantly compared to the nicotine control group (P < 0.01). Conclusion: Curcumin administration can improve hippocampal CA1 region injury induced by nicotine.

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“…We hypothesized that combined treatment with taurine, EGCG and genistein may be an effective therapy for the treatment of liver fibrosis. Previously, we reported that combined therapy with taurine, EGCG and genistein had an obvious protective effect against alcohol-induced liver fibrosis in rats by suppressing the serum levels of fibrosis markers and hepatic hydroxyproline content, as well as inhibiting hepatic collagen deposition and protein expression of B-cell lymphoma 2, α-smooth muscle actin, TGF-β1, and others against decapentaplegic homolog 3 (SMAD-3) [16]. In the current study, we evaluated the mechanistic effect of combined taurine, EGCG and genistein therapy on hepatic fibrosis using an in vitro HSC model.…”

Section: Introductionmentioning

confidence: 99%

Combined Taurine, Epigallocatechin Gallate and Genistein Therapy Reduces HSC-T6 Cell Proliferation and Modulates the Expression of Fibrogenic Factors

Luo

Zhang

et al. 2013

IJMS

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Hepatic fibrogenesis involves the activation of hepatic stellate cells (HSCs), which synthesize excess extracellular matrix and contribute to the development of liver fibrosis. In a prior study we tested the effect of combined treatment with taurine, epigallocatechin gallate and genistein on the development of alcohol-induced liver fibrosis in vitro. In this study, the biological activity of the combination of these molecules was assessed by measuring its effect on cell proliferation, fibrosis-related gene expression, and proteomic expression profiling in the activated HSC cell line, HSC-T6. HSC-T6 cells were incubated with different concentrations of the drug combination taurine, epigallocatechin gallate and genistein. Cell proliferation was evaluated by MTT assay. Transforming growth factor β1 (TGF-β1), collagen type I (Col-I), matrix metalloproteinase-2 (MMP-2), and tissue inhibitor of metalloproteinases 1 and 2 (TIMP-1 and TIMP-2) mRNA were analyzed by semi-quantitative reverse-transcription PCR. Proteomic profiling of HSC-T6 cells was also performed by SELDI-TOF-MS. Combined drug treatment significantly inhibited cell proliferation and TGF-β1, Col-I, TIMP-1 and TIMP-2 mRNA expression in activated HSC-T6 cells, while the expression of MMP-2 mRNA increased. A total of 176 protein m/z peaks were identified. The intensities of 10 protein peaks were downregulated and two protein peaks were upregulated in HSC-T6 cells after combined drug treatment. In conclusion, combined drug treatment with taurine, epigallocatechin gallate and genistein can inhibit HSC proliferation, and impact fibrosis-related gene and protein expression. The antifibrotic effects of this drug combination may be due to its effects on the expression of fibrogenic genes.

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Combination Therapy with Taurine, Epigallocatechin Gallate and Genistein for Protection against Hepatic Fibrosis Induced by Alcohol in Rats

Cited by 16 publications

References 31 publications

Toxicological evaluation of the nicotine on CA1 and protective effects of curcuma longa: An experimental study

Toxicological evaluation of the nicotine on CA1 and protective effects of curcuma longa: An experimental study

Effect of curcumin on hippocampus dentate gyrus injury induced by nicotine in rats

Combined Taurine, Epigallocatechin Gallate and Genistein Therapy Reduces HSC-T6 Cell Proliferation and Modulates the Expression of Fibrogenic Factors

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