Combination Treatment for Inhibition of the Growth of Staphylococcus aureus with Recombinant SAP8 Endolysin and Nisin

Abstract

Staphylococcus aureus, a pathogenic species of genus Staphylococcus involved in foodborne illness always remain among the top priorities of the world major concerns. In the present study, we have used recombinant SAP8 endolysin from the bacteriophage SAP8 and commercial nisin to inhibit the viability of pathogenic S. aureus KCTC 3881 cells; however, the approach was not identified as cost-effective. A gradual decrease in the viable S. aureus KCTC 3881 cell counts was observed with an increase in the concentrations of recombinant SAP8 endolysin and nisin. However, combined treatment with recombinant SAP8 endolysin and nisin decreased the viable S. aureus KCTC 3881 cell counts in a significant manner. The combination of 0.01 µM of recombinant SAP8 endolysin with 9 IU/mL and 18 IU/mL of nisin demonstrated a promising decrease in the viable cell counts of the strain. Under the scanning electron microscope, the combination treatment with 0.01 µM of recombinant SAP8 endolysin and 18 IU/mL of nisin showed complete cellular destruction of S. aureus KCTC 3881. We propose that a combination of recombinant SAP8 endolysin and nisin could be a strong alternative to antibiotics to control the growth of S. aureus including MRSA.

Phage lytic proteins: a natural approach to agro-food safety

“…aureus [74]. More recently, Kim et al [75] achieved a significant decrease in viable S. aureus cell counts by using a combination treatment consisting of endolysin SAP8 and nisin on lettuce. Another example was provided by Chang et al [76], who applied endolysin LysSA97 with carvacrol oil against S. aureus in milk and beef.…”

Combatting Methicillin-Resistant Staphylococcus Aureus (Mrsa) in the Food Industry by Harnessing the Power of Nature: A Systematic Review

“…Moreover, bacteriocins can cause a partial activation of autolysins that allows for better activity of the endolysin (Arumugam, et al 2019). Th e combination of synthetic SAP8 endolysin and nisin can eff ectively restrain various types of Gram-positive bacteria by creating openings in the bacterial cell membrane and blocking the production of cell walls (Kim, et al 2022).…”