2021
DOI: 10.1021/acs.jafc.0c07659
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Combinatorial Engineering of Transcriptional Activators in Penicillium oxalicum for Improved Production of Corn-Fiber-Degrading Enzymes

Abstract: Enzymatic conversion of corn fiber to fermentable sugars is beneficial to improving the economic efficiency of corn processing. In this work, the filamentous fungus Penicillium oxalicum was found to secrete enzymes for efficient saccharification of un-pretreated corn fiber. Separate engineering of transcriptional activators ClrB, XlnR, and AraR led to enhanced production of different sets of lignocellulolytic enzymes. Particularly, the enzymes produced by XlnR-and AraR-engineered strains showed a synergistic e… Show more

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Cited by 23 publications
(14 citation statements)
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“…Penicillium oxalicum MCAX, an engineered strain in our laboratory ( Gao et al, 2021 ), was used to produce cellulases.…”
Section: Methodsmentioning
confidence: 99%
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“…Penicillium oxalicum MCAX, an engineered strain in our laboratory ( Gao et al, 2021 ), was used to produce cellulases.…”
Section: Methodsmentioning
confidence: 99%
“…The cellulase used in this study was produced by P. oxalicum MCAX according to the method suggested by Gao et al (2021) . The strain MCAX was cultivated in 50 ml of seed medium [g/L, wheat bran: 20, peptone: 10, glucose: 10, (NH 4 ) 2 SO 4 : 2, KH 2 PO 4 : 3, and MgSO 4 : 0.5] in 300 ml Erlenmeyer flasks for 24 h at 30°C and 200 rpm on a rotary shaker.…”
Section: Methodsmentioning
confidence: 99%
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“…Corn bran (or corn bre) is an agricultural by-product obtained from corn processing. It was well recognized that corn bran polysaccharides were very di cult to be decomposed by enzymatic hydrolysis (51)(52)(53)(54). Its recalcitrance was regarded due to the rich content of glucuronoarabinoxylan which was extensively decorated with variations of both monomeric and oligomeric substitutions (55).…”
Section: Hydrolysis Action Of Ttgh74 or Ttgh74δcbm On Pretreated Corn...mentioning
confidence: 99%