Combinatorial treatment of brain samples from sheep with scrapie using sodium percarbonate, sodium dodecyl sulfate, and proteinase K increases survival time in inoculated susceptible sheep

Harm, Tyler A.; Smith, Jodi D.; Cassmann, Eric D.; Greenlee, Justin J.

doi:10.1016/j.rvsc.2022.09.002

Cited by 1 publication

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References 43 publications

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“…Moreover, the prion protein PrP can interact with modelled RNAs (Weiss et al 1997, Mercey et al 2006. Proteinase K reduces prion protein alone or in combination with other chemicals in vitro (McKinley et al 1987, Harm et al 2022. Note that in the transfection experiments in the shoot apexes of embryos (previous section), the nucleic acids were deproteinized with proteinase K plus phenol extractions because the 58-element was expected to be of nucleic acid.…”

Section: Pipetted (Tr) and Electrophoretic (Etr) Transfection Trials ...mentioning

confidence: 99%

Reciprocal extrachromosomal inheritance in barley: the transmission of an infective element through male and female gametes

Ahokas

2024

SMST

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My backcross pollinations of 53 spontaneum barleys recurrently with cv. ‘Adorra’ resulted in a maternally inherited, partial spike fertility case assigned “58”. Abnormal kernels, embryos and pollen, triploidy, miniature and streaked plants were among other symptoms. The 58-element greatly enhanced expression of the P-43 multi-ovary mutant in cv. ‘Paavo’. Cv. ‘Sudan’ was a dominant 58-sterility enhancer. Pollinating of lymegrass with 58-barley resulted in lymegrass monoploids, in which 58-specific DNA was not detected. The 58-Adorra usually showed increased RNA levels and RdRp activities. Germinating in ribavirin and in etoricoxib reduced the 58-sterility. Transfections of the shoot apexes of embryos with deproteinized 58-nucleic acids did not result in infection. Any plasmid or virus explaining the 58-symptoms could not be detected. Electrophoresed germ proteins showed a glycosylated protein in 58-Adorra (Mr approximately 78.000) not observed in Adorra. The 58-element has features of a prion. The fallout from the Soviet Chernobyl brought hot particle radioactivity to Finland before the 1986 spring sowing, and impaired this study intensively going on between 1982 and 1989.

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Section: Pipetted (Tr) and Electrophoretic (Etr) Transfection Trials ...mentioning

confidence: 99%

Reciprocal extrachromosomal inheritance in barley: the transmission of an infective element through male and female gametes

Ahokas

2024

SMST

View full text Add to dashboard Cite

show abstract

Combinatorial treatment of brain samples from sheep with scrapie using sodium percarbonate, sodium dodecyl sulfate, and proteinase K increases survival time in inoculated susceptible sheep

Cited by 1 publication

References 43 publications

Reciprocal extrachromosomal inheritance in barley: the transmission of an infective element through male and female gametes

Reciprocal extrachromosomal inheritance in barley: the transmission of an infective element through male and female gametes

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