Combinatorial variation in coding and promoter sequences of genes at the Tri locus in Pisum sativum accounts for variation in trypsin inhibitor activity in seeds

Page, David C.; Aubert, Grégoire; Duc, Gérard; Welham, Tracey; Domoney, Claire

doi:10.1007/s00438-002-0667-4

Cited by 37 publications

(27 citation statements)

References 26 publications

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“…The markers based on the Tri locus distinguish genes from pea lines that are genetic variants for quantitative expression of the seed trypsin-chymotrypsin inhibitors. Such markers promise to be reasonably robust and to circumvent the requirement for cumbersome assays of seeds in breeding programs and should pave the way for large-scale screening for seed quality parameters (Page et al, 2002). Furthermore, these studies provide some explanation for variation in quantitative expression of TI proteins.…”

Section: Legumes Also Contain Antinutritional Proteinsmentioning

confidence: 99%

Can We Improve the Nutritional Quality of Legume Seeds?

et al. 2003

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Section: Legumes Also Contain Antinutritional Proteinsmentioning

confidence: 99%

Can We Improve the Nutritional Quality of Legume Seeds?

et al. 2003

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“…Proximity of these QTL to agronomically important traits including vine length which is important for lodging resistance and anti-nutritional factors such as trypsin inhibitors have the potential to complicate selection. A set of PCR primers suitable for breeding for low trypsin inhibitor activity was developed and may alleviate challenges in breeding [211,212].…”

Section: Markers For Flowering Timementioning

confidence: 99%

Pea (Pisum sativum L.) in the Genomic Era

Smýkal

Aubert²,

Burstin³

et al. 2012

Agronomy

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Pea (Pisum sativum L.) was the original model organism used in Mendel's discovery (1866) of the laws of inheritance, making it the foundation of modern plant genetics. However, subsequent progress in pea genomics has lagged behind many other plant species. Although the size and repetitive nature of the pea genome has so far restricted its sequencing, comprehensive genomic and post genomic resources already exist. These include BAC libraries, several types of molecular marker sets, both transcriptome and proteome datasets and mutant populations for reverse genetics. The availability of the full genome sequences of three legume species has offered significant opportunities for genome wide comparison revealing synteny and co-linearity to pea. A combination of a candidate gene and colinearity approach has successfully led to the identification of genes underlying agronomically important traits including virus resistances and plant architecture. Some of this knowledge has already been applied to marker assisted selection (MAS) programs, increasing precision and shortening the breeding cycle. Yet, complete translation of marker discovery to pea breeding is still to be achieved. Molecular analysis of pea collections has shown that although substantial variation is present within the cultivated genepool, wild material offers the possibility to incorporate novel traits that may have been inadvertently eliminated. Association mapping analysis of diverse pea germplasm promises to identify genetic variation related to desirable agronomic traits, which are historically difficult to breed for in a traditional manner. The availability of high throughput 'omics' methodologies offers great promise for the development of novel, highly accurate selective breeding tools for improved pea genotypes that are sustainable under current and future climates and farming systems.

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“…These data support the development and use of molecular marker techniques to facilitate breeding programmes for grain legume improvement. 17,18 …”

Section: Trialmentioning

confidence: 99%

“…The genetic linkage of quantitative and qualitative variation has led to the development of molecular tools to assist the breeding and selection of varieties with low TI. 17,18 Although the mode of action of TI within in vitro systems has been established, it has often proved difficult to associate TI levels in peas with nutritional/physiological consequences. It has been concluded 19 that TI are not solely responsible for limiting protein digestibility in poultry and pea protein fractions resistant to digestion in poultry, albeit at low levels, have been detected.…”

Section: Introductionmentioning

confidence: 99%

The effects of genetic variation at r, rb and Tri loci in Pisum sativum L. on apparent ileal digestibility of amino acids in young broilers

et al. 2005

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The nutritional effect of genetic variation at r and rb loci was investigated by determining apparent and true amino acid digestibility of four near-isogenic lines of pea (RRRbRb, RRrbrb, rrRbRb, rrrbrb) fed to young broilers in a metabolism study. The results demonstrated that the round-seeded line (RRRbRb) generally had the lowest amino acid content with, for example, 2.5 g cystine kg −1 dry matter (DM) and 1.6 g methionine kg −1 DM, compared with contents in the three wrinkled-seeded lines (rrRbRb, RRrbrb and rrrbrb). The double mutant line (rrrbrb) generally had the highest amino acid content, with 3.2 g cystine kg −1 DM and 2.3 g methionine kg −1 DM, and an elevated amino acid content compared with the single mutant lines (RRrbrb, rrRbRb). However, the round-seeded line (RRRbRb) consistently had the highest coefficient of true (CTID), and apparent (CAID), ileal digestibility for all amino acids (e.g. 0.959 and 0.928 respectively for CTID and CAID for methionine) when compared with the three wrinkled lines RRrbrb, rrRbRb and rrrbrb (0.853, 0.877 and 0.866, respectively, for CTID, and 0.862, 0.882 and 0.874 for CAID). A second experiment examined the digestibility of amino acids in a pair of lines, HC8 and LC8, near-isogenic except for the locus controlling the concentration of seed trypsin inhibitor (H, high; L, low). The results showed that the CAID and CTID obtained for all amino acids were consistently higher for LC8 than for HC8 (for methionine: 0.905 and 0.709 for CTID, respectively, for LC8 and HC8; 0.887 and 0.729, respectively, for CAID), indicating the very specific effects of quantitative variation in these proteins on nutritional value of peas for poultry. INTRODUCTIONPea genotypes show a wide variation in seed composition, reflecting different genetic backgrounds. The genetic loci with the greatest influence on seed composition and, by inference, nutritional value include the r, rb and Tri loci, with major primary effects on starch composition (r, rb) and trypsin inhibitor content (Tri) of mature seeds.Mutations at both the r and rb loci were first identified by their effect on the shape of the mature seed, the presence of either mutation giving the dried seed a wrinkled appearance. A mutant at the r locus has been known for at least 150 years and was used by Mendel. A mutant at the rb locus was first genetically characterised 1 and it was determined that the r and rb mutations were at independent loci. The primary effects of mutations at either or at both of these

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Combinatorial variation in coding and promoter sequences of genes at the Tri locus in Pisum sativum accounts for variation in trypsin inhibitor activity in seeds

Cited by 37 publications

References 26 publications

Can We Improve the Nutritional Quality of Legume Seeds?

Can We Improve the Nutritional Quality of Legume Seeds?

Pea (Pisum sativum L.) in the Genomic Era

The effects of genetic variation at r, rb and Tri loci in Pisum sativum L. on apparent ileal digestibility of amino acids in young broilers

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