Combine effect of Chondroitinase ABC and low level laser (660 nm) on spinal cord injury model in adult male rats

Janzadeh, Atousa; Sarveazad, Arash; Yousefifard, Mahmoud; Dameni, Sima; Samani, Fazel Sahraneshin; Mokhtarian, Kobra; Nasirinezhad, Farinaz

doi:10.1016/j.npep.2017.06.002

Cited by 48 publications

(37 citation statements)

References 52 publications

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“…Only animals which had a BBB score less than 3 would be included in the study. BBB score was recorded for all the animals for 4 weeks [39].…”

Section: Behavioral Assessmentmentioning

confidence: 99%

“…Cavity size for each slide was recorded by Ima-geJ software, and eventually, the mean cavity size for each group was measured. According to our previous studies [40], the cavity size was presented as percent of the total area of the section using the following formula: Hematoxylin and eosinophil (H&E) assay H&E staining was performed for assessment of fibroblast invasion into cavity of the injury site. After staining, digital images were captured (Olympus, magnification × 40) and invasion of fibroblast was quantified manually via ImageJ software.…”

Section: Cavity Size and Myelination Assaymentioning

confidence: 99%

“…Images were captured from each section (red due to DiI and green due to secondary antibody) under fluorescent microscope (magnification × 10). According to our previous studies [40,41], the reactive area (fluorescent) was identified and the area was measured using ImageJ software based on the following formula: (Table 2).…”

Section: Immunohistochemistrymentioning

confidence: 99%

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Co-administration of human adipose-derived stem cells and low-level laser to alleviate neuropathic pain after experimental spinal cord injury

et al. 2019

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Background: Evidence has suggested that human adipose-derived stem cells (hADSCs) and low-level laser has neuroprotective effects on spinal cord injury (SCI). Therefore, the combined effect of the hADSCs and laser on neuregeneration and neuropathic pain after SCI was investigated. Methods: Forty-eight adult male Wistar rats with 200-250 g weight were used. Thirty minutes after compression, injury with laser was irritated, and 1 week following SCI, about 1 × 10 6 cells were transplanted into the spinal cord. Motor function and neuropathic pain were assessed weekly. Molecular and histological studies were done at the end of the fourth week. Results: The combined application of hADSCs and laser has significantly improved motor function recovery (p = 0.0001), hyperalgesia (p < 0.05), and allodynia (p < 0.05). GDNF mRNA expression was significantly increased in hADSCs and laser+hADSC-treated animals (p < 0.001). Finally, co-administration of hADSCs and laser has enhanced the number of axons around cavity more than other treatments (p < 0.001). Conclusions: The results showed that the combination of laser and ADSCs could significantly improve the motor function and alleviate SCI-induced allodynia and hyperalgesia. Therefore, using a combination of laser and hADSCs in future experimental and translational clinical studies is suggested.

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“…Only animals which had a BBB score less than 3 would be included in the study. BBB score was recorded for all the animals for 4 weeks [39].…”

Section: Behavioral Assessmentmentioning

confidence: 99%

Section: Cavity Size and Myelination Assaymentioning

confidence: 99%

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Co-administration of human adipose-derived stem cells and low-level laser to alleviate neuropathic pain after experimental spinal cord injury

et al. 2019

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“…In our previous study where LLL therapy was performed for two weeks, it was indicated that although a lowlevel laser can reduce the expression of Chondroitin sulfate, an impenetrable material forming of a glial barrier, it caused the entrance of fibroblasts into and around the injury (18). After mechanical damage to the spinal cord, the injured part is susceptible for the second phase and is separated from the healthy part by forming a glial wound (29).…”

Section: Discussionmentioning

confidence: 99%

The Effect of Four Weeks of Low-Level Laser Radiation (660 nm) on Movement Recovery and Fibroblasts Invasion

et al. 2019

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Background: Despite the fact that many researches have been done in regards to spinal cord injury (SCI), it remains an undissolved problem that has an effect on the individual's life and society. It could be due to complicated events that occur after SCI. Inflammation is an important initiator for after SCI events and low-level laser (LLL), which is known as an anti-inflammatory agent. Objectives: The aim of this study is the evaluation effects of LLL on the fibroblast invasion, axon number, and functional recovery after SCI. Methods: Twenty-four adult male Wistar rats were randomly divided into three groups as control, SCI, SCI + laser. Thirty minutes after SCI that was induced by aneurysm clip LLL irritated for 45 seconds daily and continued for four weeks. Functional recovery by BBB score, fibroblast invasion by H&E, and Axons number by Bielschowsky silver staining was assessed. Results: The data indicated LLL therapy improved BBB score, increased axons number, and decreased fibroblasts invasion around cavity comparing to SCI animals (P < 0.001). Conclusions: Our result showed that the initiation LLL therapy, in a short time after SCI, could be effective in the reduction of SCI side effects and improved functional recovery.

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“…In recent years, PBM has been broadly applied in therapy for brain injury and other cerebral diseases such as stroke and neurodegenerative disorders, where it has yielded encouraging results . Similarly, research on SCI has revealed that PBM can suppress the activation of immune cells at the site of injury, reduce the expression of proinflammatory factors and chemokines and reduce the incidence of secondary SCI, as well as promote the large‐scale regrowth of nerve fibers at the site of injury and significantly improve the recovery of locomotor function . Previously, we discovered that irradiating SCI sites by percutaneous in rats with near‐infrared light at 810 nm, 150 mW, 1500 J/cm 2 can promote macrophage polarization from the M1 to the M2 phenotype, reduce the immunoinflammatory response, promote the survival of neurons and improve the recovery of locomotor function .…”

Section: Introductionmentioning

confidence: 99%

Photobiomodulation by diffusing optical fiber on spinal cord: A feasibility study in piglet model

Liang

Tao

Wang

et al. 2020

Journal of Biophotonics

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Combine effect of Chondroitinase ABC and low level laser (660 nm) on spinal cord injury model in adult male rats

Cited by 48 publications

References 52 publications

Co-administration of human adipose-derived stem cells and low-level laser to alleviate neuropathic pain after experimental spinal cord injury

Co-administration of human adipose-derived stem cells and low-level laser to alleviate neuropathic pain after experimental spinal cord injury

The Effect of Four Weeks of Low-Level Laser Radiation (660 nm) on Movement Recovery and Fibroblasts Invasion

Photobiomodulation by diffusing optical fiber on spinal cord: A feasibility study in piglet model

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